PALB2 as Another Candidate Gene for Genetic Testing in Patients with Hereditary Breast Cancer in Czech Republic

Česká verzia

Authors: M. Janatová ¹; M. Borecká ¹; J. Soukupová ¹; P. Kleiblová ¹; J. Stříbrná ¹; M. Vočka ²; P. Zemánková ¹; A. Panczak ³; K. Veselá ³; P. Souček ⁴; L. Foretová ⁵; Z. Kleibl ¹
Authors place of work: Ústav biochemie a experimentální onkologie, 1. LF UK v Praze ¹; Onkologická klinika 1. LF UK a VFN v Praze ²; Ústav biologie a lékařské genetiky, 1. LF UK a VFN v Praze ³; Oddělení toxikogenomiky, Státní zdravotní ústav, Praha ⁴; Oddělení epidemiologie a genetiky nádorů, Masarykův onkologický ústav, Brno ⁵
Published in the journal: Klin Onkol 2016; 29(Supplementum 1): 31-34
Category: Original Articles
doi: https://doi.org/10.14735/amko2016S31

Summary

Background:
The PALB2 (FANCN) gene was identified as a component of endogenous BRCA2 complex that encodes a DNA repair protein participating along with BRCA1 and BRCA 2 proteins in DNA double-strand break repair. Hereditary PALB2 mutations are associated with an increased risk of breast and pancreatic cancers in heterozygotes. Breast cancer risk for PALB2 mutation carriers has recently been estimated at 33–58% depending on family history of breast cancer; pancreatic cancer risk in carriers of PALB2 mutations has not been precisely quantified, yet.

Materials and Results:
Results of a study identifying PALB2 mutations in high-risk, BRCA1/2-negative, breast and/or ovarian cancer patients in the Czech Republic indicate that the frequency of hereditary PALB2 mutations in our population is quite high. Interestingly, almost 20% of all recognized mutations comprised large genomic rearrangements. The highest proportion of PALB2 mutations (comparable with the number of mutations reported for BRCA2) was found in a subgroup of hereditary breast cancer patients (5.5%). Frequency of mutations in an independent group of Czech unselected pancreatic cancer patients was approximately 1.3%.

Conclusion:
Considering the frequency of pathogenic, hereditary PALB2 mutations in our population, their phenotypic similarity to BRCA2, and expected risk of breast cancer associated with PALB2 mutations, its screening (including large genomic rearrangements) should be encouraged in patients from hereditary breast cancer families. The follow-up of pathogenic PALB2 mutation carriers should be similar to that in BRCA2 mutation carriers, enabling early diagnosis, prevention, and possible targeted therapy. Preventive surgical interventions for the carriers could be considered in case of strong family cancer history and evident segregation of a pathogenic mutation with a tumor phenotype. Additional analysis of various cancer patient populations and further meta-analyses will be necessary for accurate assessment of PALB2 gene penetrance and its significance for the risk of pancreatic and other cancers.

Key words:
hereditary breast and ovarian cancer syndrom – pancreatic neoplasms – PALB2 gene – genetic predisposition – genetic testing

This study was supported by grants IGA MH CZ NT14006-3/2013 and NT13343-4/2012, and Charles univessity in Prague – PRVOUK-P27//LF1/1 a SVV-UK 260148/2015.

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE recommendation for biomedical papers.

Submitted:
6. 8. 2015

Accepted:
14. 8. 2015

Zdroje

1. Pohlreich P, Zikan M, Stribrna J et al. High proportion of recurrent germline mutations in the BRCA1 gene in breastand ovarian cancer patients from the Prague area. Breast Cancer Res 2005; 7(5): R728– R736.

2. Soukupova J, Dundr P, Kleibl Z et al. Contribution of mutations in ATM to breast cancer development in the Czech population. Oncol Rep 2008; 19(6): 1505– 1510.

3. Kleibl Z, Novotny J, Bezdickova D et al. The CHEK2 c.1100delC germline mutation rarely contributes to breast cancer development in the Czech Republic. Breast Cancer Res Treat 2005; 90(2): 165– 167.

4. Ticha I, Stribrna J, Soukupova J et al. Mutations in CHEK2 and TP53 genes in high‑risk hereditary breast and ovarian cancer patients in the Czech Republic. Eur J Cancer Suppl 2011; 47 (Suppl 1): S132– S132.

5. Mateju M, Kleiblova P, Kleibl Z et al. Germline mutations 657del5 and 643C>T (R215W) in NBN are not likely to be associated with increased risk of breast cancer in Czech women. Breast Cancer Res Treat 2012; 133(2): 809– 811. doi: 10.1007/ s10549‑ 012‑ 2049‑ x.

6. Xia B, Sheng Q, Nakanishi K et al. Control of BRCA2 cellular and clinical functions by a nuclear partner, PALB2. Mol Cell 2006; 22(6): 719– 729.

7. Zhang F, Ma J, Wu J et al. PALB2 links BRCA1 and BRCA2 in the DNA‑ damage response. Curr Biol 2009; 19(6): 524– 529. doi: 10.1016/ j.cub.2009.02.018.

8. Bartek J, Bartkova J, Lukas J. DNA damage signalling guards against activated oncogenes and tumour progression. Oncogene 2007; 26(56): 7773– 7779.

9. Reid S, Schindler D, Hanenberg H et al. Biallelic mutations in PALB2 cause Fanconi anemia subtype FA‑ N and predispose to childhood cancer. Nat Genet 2007; 39(2): 162– 164.

10. Tischkowitz M, Xia B. PALB2/ FANCN: recombining cancer and Fanconi anemia. Cancer Res 2010; 70(19): 7353– 7359. doi: 10.1158/ 0008‑ 5472.CAN‑ 10‑ 1012.

11. Southey MC, Teo ZL, Winship I. PALB2 and breast cancer: ready for clinical translation! Appl Clin Genet 2013; 19(6): 43– 52. doi: 10.2147/ TACG.S34116.

12. Rahman N, Seal S, Thompson D et al. PALB2, which encodes a BRCA2– interacting protein, is a breast cancer susceptibility gene. Nat Genet 2007; 39(2): 165– 167.

13. Antoniou AC, Casadei S, Heikkinen T et al. Breast‑ cancer risk in families with mutations in PALB2. N Engl J Med 2014; 371(6): 497– 506. doi: 10.1056/ NEJMoa1400382.

14. Janatova M, Kleibl Z, Stribrna J et al. The PALB2 gene is a strong candidate for clinical testing in BRCA1- and BRCA2- negative hereditary breast cancer. Cancer Epidemiol Biomarkers Prev 2013; 22(12): 2323– 2332. doi: 10.1158/ 1055‑ 9965.EPI‑ 13‑ 0745‑ T.

15. Couch FJ, Hart SN, Sharma P et al. Inherited mutations in 17 breast cancer susceptibility genes among a large triple‑ negative breast cancer cohort unselected for family history of breast cancer. J Clin Oncol 2015; 33(4): 304– 311. doi: 10.1200/ JCO.2014.57.1414.

16. Jones S, Hruban RH, Kamiyama M et al. Exomic sequencing identifies PALB2 as a pancreatic cancer susceptibility gene. Science 2009; 324(5924): 217. doi: 10.1126/ science.1171202.

17. Becker AE, Hernandez YG, Frucht H et al. Pancreatic ductal adenocarcinoma: risk factors, screening, and early detection. World J Gastroenterol 2014; 20(32): 11182– 11198. doi: 10.3748/ wjg.v20.i32.11182.

18. Bhalla A, Saif MW. PARP‑inhibitors in BRCA‑associated pancreatic cancer. JOP 2014; 15(4): 340– 343. doi: 10.6092/ 1590‑ 8577/2690.