Non-melanoma Skin Cancer – a Clinicopathological Study of Patients with Basal Cell Carcinoma and Squamous Cell Carcinoma

Summary

Background:
Non-melanoma skin cancer (NMSC) is the most common malignancy in Caucasians. It mainly includes two major keratinocyte tumors – basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). The objective of the study was to analyze and compare the clinicopathological differences between patients with BCC and SCC of the skin.

Material and Methods:
A cohort of 541 patients with a total of 719 BCCs, and 126 patients with a total of 162 SCCs were retrospectively analyzed.

Results:
While there was virtually the same proportion of men (49.91%) and women (50.09%) in BCC patients, SCCs occurred more frequently in men (68.2%) than in women (31.8%). The mean age of the individuals with BCC and SCC was 70.8 and 78.2 years, resp. The number of BCCs rises from 50 years of age and this increase showed a linear trend up to 80 years, subsequently followed by decline. SCC lesions occur more rapidly from 70 years of age followed by a sharp increase that exhibited an exponential relationship. BCCs and SCCs occurred predominantly on the head and neck region, comprising a total of 69.8% and 81.4% of the cases, resp. However, BCC lesions were seen more often on the face and SCC lesions were diagnosed more frequently on the extra-facial parts of the head. Further, BCCs occurred more frequently on the trunk, and particularly on the back, compared to SCCs.

Conclusion:
Although BCC and SCC are covered under common term NMSC, they manifest several clinicopathological differences. Despite sharing common etiologic determinants, at least from the onco-epidemiologic perspective, they should be considered separately.

Key words:
non-melanoma skin cancer – basal cell carcinoma – squamous cell carcinoma

Introduction

Non-melanoma skin cancer (NMSC) is currently the most common malignancy in Caucasians with continuing increase in incidence worldwide [1,2]. Although the term NMSC covers all cutaneous neoplasias that do not originate from melanocytes, it is widely used to refer to two major types of keratinocyte tumors – basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) [1,2]. These two forms of cancer account for more than 95% of all NMSC. BCC comprises about 80% of the cases, and it is by far the most frequent malignancies diagnosed in people of Caucasian ancestry [1]. In contrast to other neoplasms, BCC generally pursues a favorable clinical course, growing only locally and having minimal (virtually none) metastatic potential. However, some lesions may exhibit an aggressive behavior with a deep tissue infiltration accompanied by repeated recurrences after treatment [3,4]. SCC is less frequent, representing approximately 16% of all NMSC [1]. Its prognosis is slightly worse, as it may sometimes metastasize (0.3–3.7%) and cause death [2], but this is also a relatively rare finding in a routine clinical practice. In general, despite the fact that overall mortality of BCC and SCC is very low, there may be substantial morbidity due to impossible adequate cure of the lesions and visual disfigurement, as the tumors tend to be located mostly on the head, especially on the face.

Etiological factors that underlie the development of NMSC are multiple and interrelated. They include environmental, phenotypic and genetic determinants [2]. There is persuasive evidence that the key exogeneous (environmental) risk factor for both, BCC and SCC, is ultraviolet radiation (UVR). The others include e. g. arsenic or tar exposure, chronic dermatitis, other chronic dermatoses or HPV infection of the skin, most of which participate especially in SCC pathogenesis [2]. The important phenotypic features associated with increased risk of NMSC include fair or red hair, blue eyes, and Fitzpatrick skin type I and II [2]. Among hereditary conditions, the prototypic disease in which the subjects are susceptible to develop numerous BCCs is Gorlin syndrome [2,5,6]. Another rare genetic disorder, where the persons are sensitive to sunlight and strongly predisposed to various skin malignan-cies, is xeroderma pigmentosum [2,5]. As both carcinomas share similar etiopathogenesis, clinical features and prognosis, they have almost identical treatment strategies and clinical management. On the other hand, certain epidemiologic and clinicopathological aspects differ between them. For example, a development of BCC and SCC is influenced by distinct patterns of UVR exposure [2,7]. Further, while sporadic BCCs usually arise de novo without any precursor lesion, the majority (over 72%) of SCCs result from solar keratosis or actinically damaged skin [2]. Reduced immunity (e. g. immunosuppression in organ transplant recipients) is more linked to risk of SCC, especially with an evolution of multiple lesions [8,9]. On the contrary, development of multiple sporadic BCCs appears to be much more related to individual genetic predisposition [10].

The objective of the present study was to analyze and compare the clinicopathological differences between patients with BCC and SCC of the skin. We compared our results with relevant literature data in this field.

Material and methods

The electronic database of Department of Pathology in Faculty Hospital in Žilina was searched using the ICD-10 (International Statistical Classifica-tion of Diseases and Related Health Problems – 10^th revision) codes C44.0-9 and D04.0-9 as the keywords to locate all histopathology reports with a diagnosis of NMSC from January 1, 2010 to June 30, 2017. Among all cases obtained, only diagnoses of BCC, invasive SCC and in situ SCC were accepted. Further, recurrent lesions and subsequent re-excisions after incomplete tumor removal were excluded. Finally, two separate subsets of the individuals with BCC and SCC (both in situ and invasive forms) were gathered and prepared for analysis. The tissue specimens were derived from a variety of clinical sources at our hospital (i.e. departments of surgery, dermatology, otorinolaryngology and ophtalmology). Biopsy material was fixed in buffered formalin, embedded in paraffin blocks and stained with hematoxylin and eosin. Medical records of included patients were reviewed to gain basic clinical informations, such as the number of tumors per person, gender, age at the time of diagnosis, topographic locations of lesions and local recurrences arising during the study period. Analysis of histopathologic subtypes and variants of BCC and SCC was not part of the scope of the study. The BCC/SCC ratio was calculated as a ratio between the numbers of the given tumor lesions. Data were collected in a databank, using a software SPSS Statistics. For statistical analysis, chi-square test was employed and p < 0.05 was considered significant.

Results

A cohort of consecutive 541 patients (270 males, 271 females) with a total of 719 primary BCCs and consecutive 126 patients (86 males, 40 females) with a total of 162 SCCs (47 in situ, 115 invasive lesions) were included in this study. Among them, 33 subjects (25 males, 8 females) had the synchronous or metachronous occurrence of both carcinoma types. The overall BCC/SCC ratio was 4.4: 1. While there was virtually the same proportion of men (49.91%) and women (50.09%) in BCC patients, SCCs occured much more frequently in men (68.2%) than women (31.8%), attaining statistical significance (p < 0.001). Patients with BCCs ranged between 23 and 97 years of age (mean 70.8 years with a standard deviation of 12.7, median 71 years). The age group mostly affected by BCC was 71–80 years. Patients with SCCs ranged between 34 and 95 years (mean 78.2 years with a standard deviation of 9.7, median 80 years). The age group mostly affected by SCC was 81–90 years. Although both carcinomas were diagnosed mainly in older individuals, there were different patterns in increasing trend of tumor occurrence in relation to age. As for BCC, a number of lesions began to rise apparently from 50 years of age, and this increase showed a linear trend up to 80, subsequently followed by decline. On the other hand, SCC lesions started to occur more rapidly from 70 years of age followed by a sharp increase that exhibited an exponential relationship (Graph 1). As a result, marked differences in the male to female (M/F) ratio between BCC and SCC subjects in relation to individual age categories were found. While the M/F ratio was 26: 1 in the youngest population (≤ 50 years), it has been gradually decreasing with rising age and reached a value of 1.6: 1 in the oldest persons over 90 years (Tab. 1). There were only two SCC patients younger than 50; of these patients, the youngest one (35-year-old female) had a single in situ SCC arising in chronic psoriatic plaque.

As for topographic distribution, BCCs and SCCs occurred predominantly on the head and neck region, comprising 69.8% (n = 502) and 81.4% (n = 132) of the cases, resp. However, BCC lesions were seen more often on the face, compared to SCC (p = 0.01) and vice versa, SCC lesions were diagnosed much more frequently on the extra-facial parts of the head (p < 0.0001). Further, BCCs occurred more frequently (p = 0.003) on the trunk, and particularly on the back (p < 0.0001), compared to SCCs. No significant differences between BCC and SCC lesions regarding the other anatomic positions, such as the neck (p = 0.8), upper extremities (p = 0.06) and lower extremities (p = 0.1) were found.

Multiple primary lesions (two and more carcinomas of the corresponding type to define patients΄ subset) were found in 18.6% (n = 30) and 15.8% (n = 3) of all BCC and SCC individuals, resp. In both subroups, men were more prone to develop multiple tumors, but this was found statistically significant only for BCC (p = 0.01).

In the given study period, there were 30 cases (4.0%) of biopsy proven BCC recurrences and mere 3 cases (1.8%) of biopsy proven SCC recurrences. Although the percentage was considerably higher in BCC, because only a few cases of SCC relapsed, the statistical analysis was not performed. No lymph node or distant metastases were found in both carcinoma types during the whole 7.5-year study period.

A summary of the clinicopathological parameters in both patients’ subsets we investigated in this study is illustrated in Tab. 2.

Discussion

In the present research, we evaluated and compared available clinicopathological findings in patients’ cohorts who suffered from two most common malignant skin neoplasms. This study included 541 patients with BCCs and 126 patients with SCCs. In accordance with earlier Slovak analysis published in 2000 [1], a prevalence of BCC was more than four times higher compared to SCC, resulting in BCC/SCC ratio of 4.4: 1. Similar values were documented in another report from European countries [11,12]. With regard to the location of tumors, most of the studies published until now have shown that the head and neck region was the most frequently affected body site. It represented 68.4–87% of all BCCs [1,12–16] and 70.2–77.6% of all SCCs [1,12,17] analyzed. In the cur-rent study, this anatomic part comprised 69.8% of BCCs and 81.4% of SCCs diagnosed, corroborating the papers mentioned above. Despite the fact, both skin malignancies occurred predominantly on the body sites, which were permanently exposed to sunlight, BCC lesions developed much more frequently on the trunk, especially on the back, compared to SCCs. These data are similar to those found in the literature [1,11,12,18]. It is in agreement with general idea that a chronic (lifetime) exposure to solar UVR plays a major role in SCC development, an intermittent UVR (particularly in childhood and adolescence) seems to be more prejudical etiologic determinant for BCC formation [2,7]. It is also in parallel with a finding that increasing age (especially from the seventh decade of life) is accompanied by much higher probability of SCC development, as we illustrated in Tab. 2. As a result, BCC/SCC ratio becomes gradually lower with age, because an increase in risk of tumor development tends to be linear for BCC, but exponential for SCC. In addition, most SCC lesions arise from actinically damaged skin, which is an indicator of cumulative (lifetime) UVR exposure [2]. On the contrary, an association between morphologic markers of cutaneous photodamage and an increased risk of BCC has been proven to be only moderate [19].

Regarding gender, we found that SCCs more frequently occurred in men (68.2%) than women (31.8%), justifying data from many previous reports [1,11,12,17,18]. In BCC, however, the results regarding gender prevalence are much more controversial. While some authors [1,13,14,16] have demonstrated a higher preponderance of men, other investigators [15,18] found higher prevalence of women. In our series, a proportion of men (49.91%) and women (50.09%) affected by BCC was virtually identical. Similar observations have been recently reported by Andrese et al. [12] in North East of Romania. Here it should be mentioned that the traditional incidence pattern, where BCC was more frequently seen in older patients, usually males, seems to be changing. For example, the Dutch [20] and Danish [21] researchers have recently observed marked increase in BCC incidence in younger women younger than 40. Even data from Great Britain [22] documented that between years 2004 and 2010, the largest average increase in incidence had been observed amongst those aged 30–49 years. During this time, there was a slightly higher increase in BCC incidence in women. The present data suggest that the “typical” BCC patient in northwestern Europe is becoming younger and is more often female. This inversion of prevalence may be associated with new concept of beauty, such as the “culture of tanned skin” with a rising demand for natural and artificial tanning in young women [14]. Further, it could be at least partially explained by the fact that the female population is the target audience for educational campaigns against cancer, making them more aware and careful about their own body [14,16].

A considerable percentage of BCC and SCC subjects had multiple primary tumor lesions, principally the men. The differences between these two carcinoma types were not statistically significant in our study. Multiple (simul-taneous or metachronous) development of cutaneous keratinocyte carcinomas is a well-known phenomenon and it is probably the result of both, exogeneous and endogeneous etiologic determinants. According to most literature reports, the proportion of patients with more than one primary BCC represents up to 28% of the cases [16,23,24]. The situation in SCC seems to be similar. In the papers published by Stang et al. [25] and Levine et al. [9], 14% and 26.1% of the SCC patients suffered from more than one primary SCC lesion during the 5-and 10-years study periods, resp. In our files, the percentage of patients with multiple BCCs (18.6%) was lower compared to the above-mentioned reports [16,23,24]. The main reason may be that we only determined a proportion of the individuals with multiple BCCs among defined patients’ cohorts with-out their precise follow-up. We dealt with this issue in our recent publication in detail [26]. The percentage of persons with multiple primary SCCs (15.8%) was almost the same as in the study published by Stang et al. [25]. Although it has been previously shown [8,9] that subjects with multiple SCCs were more likely to be immunosuppressed, it was beyond our possibilities to monitor their personal immune status. From a practical point of view, however, once an individual develops NMSC, there is increased risk that a new skin keratinocyte cancer will appear in the next few years. Therefore, all patients with NMSC should be followed-up after treatment. In contrast to many other human malignancies, a secondary prevention of skin cancers (in the form of a total body skin exams) is noninvasive and easily applicable.

Since NMSC only very sporadically leads to metastases, one of the most adverse clinical features of disease is local tumor recurrence. This is a relatively frequent matter in a routine dermatologic practice. The recurrence rate depends on several factors, such as anatomic location and size of lesions, histologic subtypes of tumors, chosen therapeutic modality and resection margin status after total surgical excision. According to literature data, local recurrence rates range between 3.4 and 4.3% for BCCs [27,28] and between 2 and 3.6% for SCCs [27,29,30]. These values are similar and even some authors [27] did not confirm a statistical significance between BCC and SCC recurrence rates. In the present study, 4.1% of all BCC lesions and 1.8% of all SCC lesions locally recurred during the 7.5-years period, roughly agreeing with the observations from previous studies [27–30]. However, as they represented only microscopically verified cases, a certain number of tumor relapses could have been subsequently treated non-invasively without histopathologic examination.

Conclusion

Although BCC and SCC are covered under common term NMSC, they manifest several clinicopathological differences. In particular, the most important ones include distinct gender and age predominance and detailed body site distribution. The described variances reinforce the fact that they represent distinct nosologic entities, resulting from different oncogenic pathways. Despite sharing common etiologic determinants, at least from the onco-epidemiologic perspective, they should be considered separately.

Submitted: 7. 8. 2017

Accepted: 25. 9. 2017

The authors wish to thank MUDr. Oľga Zacharová, MUDr. Dušan Pokorný and MUDr. Jana Doboszová for their educational support and technical assistance.

The authors declare no conflict of interests regarding the publication of this paper.

The Editorial Board declares that the manuscript met the ICMJE recommendation for biomedical papers.

MUDr. PhDr. Vladimír Bartoš, PhD.

Faculty Hospital with polyclinic Žilina

Vojtecha Spanyola 43

012 07 Žilina Slovakia

e-mail: vladim.bartos@gmail.com