Ononis arvensis in vitro –Abiotic Elicitation

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Ononis arvensis in vitro –abiotická elicitace

Byl testován vliv dvou abiotických elicitorů,a to dvou nově syntetizovaných sloučenin (substituovaných anilidů pyrazin-2-karboxylových kyselin)na tvorbu flavonoidů v kalusové kultuře Ononisarvensis L.Testované sloučeniny ovlivňovaly výrazně produkci flavonoidů v kultuře in vitro .Zejména po elicitaci roztokem látky č.I –(4-hydroxyanilid 6-chlor-5-terc.butylpyrazin-2-karboxylová kyselina)v koncentraci (3,32.10 –7 mol.l –1 )a době elicitace 48 hodin došlo ke zvýšení tvorbyflavonoidů o 976 %oproti kontrole.

Klíčová slova:
Ononis arvensis –flavonoidy –elicitace –substituce anilidů 2-karboxylovýchkyselin

Authors: L. Tůmová; P. Ostrožlík
Authors‘ workplace: Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové
Published in: Čes. slov. Farm., 2002; , 173-176
Category:

Overview

The effect of two abiotic elicitors –two newly synthetized compounds (substituted anilides ofpyrazine-2-carboxylic acids)–on the formation of flavonoids in the callus culture of Ononis arvensisL.was tested.The compounds markedly influenced the production of flavonoids in an in vitro culture.Particularly after elicitation with a solution of compound No.1 (4-hydroxyanilide 6-chloro-5-terc.butylpyrazine-2-carboxylic acid)in a concentration of 3.32.10 –7 mol.l –1 and within 48 hours of elicitation an increase in flavonoid formation by 976 %versus the control took place.

Key words:
Ononis arvensis –flavonoids –elicitation –substituted anilides of 2-carboxylic acids

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Article was published in

Czech and Slovak Pharmacy

2002 Issue 4