The new technologies for the analytical examination of the embryonic metabolome and its prospects

Česká verzia

Authors: I. Crha ¹ ; A. Mádr ²; J. Musilová ²; Z. Glatz ²; J. Žáková ¹; P. Ventruba ¹
Authors place of work: Gynekologicko-porodnická klinika LF MU a FN Brno, přednosta prof. MUDr. P. Ventruba, DrSc., MBA ¹; Ústav biochemie, Přírodovědecká fakulta a CEITEC, Masarykova univerzita, Brno, vedoucí prof. RNDr. Z. Glatz, CSc. ²
Published in the journal: Ceska Gynekol 2012; 77(6): 502-506

Summary

Objective:
To review current technologies for the analytical examination of the embryonic metabolome and its perspectives.

Design:
Review article.

Setting:
Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and University Hospital, Brno, Department of Biochemistry, Faculty of Science and CEITEC, Masaryk University, Brno.

Methods and results:
Nowadays, very sensitive analytical technologies are available. They enable exact measurement of various molecules – products of embryo metabolism during first days of cultivation. The capillary electrophoresis is one of promising method. Recent studies analysed metabolic differences between embryos that result in a pregnancy and those that do not. Amino acid levels, glucose or pyruvate in the embryo culture media were analysed most frequently. However, results of these studies are ambiguous.

Conclusions:
The capillary electrophoresis with contactless conductivity detection may provide a useful data of the embryonic metabolome. A comprehensive analysis of the used culture medium may represent a valuable adjunct to morphological criteria for enhanced rates of implantation and delivery.

Key words:
metabolomics, metabolome analysis, capillary electrophoresis, embryo, assisted reproductive techniques.

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