Detekce superantigenů u izolátů Streptococcus pyogenes na základě dat celogenomové sekvenace
Authors:
R. Veselá 1
; S. Vohrnová 1,2
; J. Kozáková 1
Authors place of work:
Státní zdravotní ústav, Centrum epidemiologie a mikrobiologie, Národní referenční laboratoř pro streptokokové nákazy
1; 3. lékařská fakulta Univerzity Karlovy, Praha
2
Published in the journal:
Epidemiol. Mikrobiol. Imunol. 72, 2023, č. 3, s. 191-194
Category:
Short Communication
Summary
Streptococcus pyogenes causes a variety of human diseases ranging from uncomplicated respiratory tract and skin infections to severe invasive diseases possibly involving toxic shock syndrome. Besides the emm gene-encoded M protein, important virulence factors are pyrogenic exotoxins, referred to as superantigens. The National Reference Laboratory for Streptococcal Infections has newly introduced bioinformatics tools for processing S. pyogenes whole genome sequencing data. Using the SRST2 software and BV-BRC platform, WGS data of 10 S. pyogenes isolates from patients with invasive disease were analysed, and emm type, sequence type, and superantigen encoding gene profiles were determined. The Unicycler assembly pipeline with the SPAdes de novo assembler was used to assemble genome sequences from short reads.
Keywords:
whole genome sequencing – Streptococcus pyogenes – GAS – superantigen
Zdroje
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Štítky
Hygiene and epidemiology Medical virology Clinical microbiologyČlánok vyšiel v časopise
Epidemiology, Microbiology, Immunology
2023 Číslo 3
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