Experimental model of perianal fistula in a small laboratory animal

Česká verzia

Authors: O. Ryska ¹; Z. Šerclová ¹; O. Měšťák ²; E. Matoušková ³; P. Veselý ²
Authors place of work: Chirurgická klinika 2. LF UK a ÚVN, Praha ¹; Klinika plastické chirurgie 1. LF UK a Nemocnice Na Bulovce, Praha ²; Klinika popáleninové medicíny 3. LF UK, Praha ³
Published in the journal: Gastroent Hepatol 2013; 67(4): 298-302
Category: Clinical and Experimental Gastroenterology: Original Article

Summary

Perianal fistula is a common disease usually requiring surgical interventions. New alternative therapeutic methods (stem cells transplantation, fibrin glue, fistula plug) are currently investigated. A suitable animal model is needed to evaluate the effect of these techniques in an experimental study. The aim of this study was to create a clinically adequate model of perianal fistula applicable in the evaluation of new therapeutic methods.

Methods:
Axial caecostomy with 2.5 cm subcutaneous channel was performed in 30 Wistar rats. After the 40-day follow-up, the animals were randomized into three groups. The first group was treated with suture to the fistula opening the second group by fistula tract ligation, and the third was a control group without intervention.

Results:
The model was technically feasible in all animals with an average operating time 22 (± 5.1) mins. During the 40-day observation two animals (7%) died on the first and fifth postoperative day, and one animal (3%) had spontaneous fistula healing. In total, 27 animals were randomized. In groups with opening suture (n = 9), tract ligation (n = 12) and in control group (n = 8) fistula healing was observed in three (33%), six (50%) and 0 (0%) animals. Results in interventional groups were in agreement with results of clinical trials.

Conclusion:
A clinically adequate model of perianal fistulas suitable for the evaluation of new treatment techniques was created.

Keywords:
perianal fistula – an animal model – rat

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE „uniform requirements“ for biomedical papers.

Submitted:
13. 7. 2013

Accepted:
7. 8. 2013

Zdroje

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