DNA analysis and histological subtypes of endometrial cancer

Česká verzia

Authors: E. Radzo ¹; P. Sýkora ¹; Ľudovít Danihel ²; V. Šišovský ²; Vanda Repiská ¹
Authors place of work: Univerzita Komenského, Bratislava, Slovenská republika Lekárska fakulta ; Ústav lekárskej biológie, genetiky a klinickej genetiky Prednosta: doc. MUDr. D. Böhmer, Ph. D. ¹; Ústav patologickej anatómie Prednosta: prof. MUDr. Ľ. Danihel, Ph. D. ²
Published in the journal: Prakt. Lék. 2010; 90(3): 171-173
Category: Of different specialties

Summary

Introduction:
The total number of carcinoma of the endometrium (CaE) has a growing global trend. Most cases of CaE are classified as endometrioid histological subtype, which generally has a good prognosis and is treatable. The serous histological subtype of CaE is a rarer form of endometrial carcinomas; it has a worse prognosis and is diagnosed at later stages of disease. Given the prognosis of serous CaE it is useful and necessary to identify this subtype of carcinomas in mixed types of CaE.

Material and Methods:
We analyzed endometrial tissue in a group of 24 patients – the patients were between 44 and 88 years old. The histological findings of the samples studied varied from simple hyperplasia without atypia to endometrioid CaE with significant infiltration into myometria. The samples were also analyzed by direct sequencing and restriction analysis.

Results:
In all of the samples from the 24 patients with endometrial cancer or precancerous lesions, we identified a CCC/CGC Pro72Arg polymorphism in Exon 4. The ratio of heterozygous and homozygous polymorphisms was 14:10. The results suggest that there may be a mixed type of endometrial cancer.

Key words:
endometrium, carcinogenesis, TP53, DNA polymorphism.

Zdroje

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