Implication of cytogenetic and molecular cytogenetic analysis in diagnosis of hematological malignancies in the era of the new sequencing techniques
Authors:
Zuzana Zemanová 1; Kyra Michalová 1; Jana Březinová 2
Authors place of work:
Centrum nádorové cytogenetiky, Ústav lékařské biochemie a laboratorní diagnostiky 1. LF UK a VFN v Praze
1; Ústav hematologie a krevní transfuze, Praha
2
Published in the journal:
Čas. Lék. čes. 2019; 158: 22-27
Category:
Review Article
Summary
In patients with hematological malignancies one of the most substantial findings is the karyotype of bone marrow cells at the time of diagnosis. The detection of clonal chromosome aberrations in diagnostic samples not only confirms a neoplastic or premalignant process but also provides important diagnostic and prognostic information essential for precise disease classification and choice of suitable therapy. Karyotype analysis during the disease course also allows monitoring of the treatment success reflected as well in the revised WHO classification where patients are often classified into the different diagnostic subtypes based on the finding of specific chromosome and/or genetic changes. Recently, also increases the number of advanced treatment approaches that directly or indirectly target the genetic aberrations present in tumor cells. Despite the large development of new sequencing technologies in recent years, cytogenetic analysis supplemented by the molecular cytogenetic methods still remains a very important part of diagnostics of hematological malignancies.
Keywords:
conventional cytogenetic analysis – molecular cytogenetic analysis – karyotype – chromosome aberrations – clonal heterogeneity
Zdroje
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Štítky
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental HygienistČlánok vyšiel v časopise
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