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preLekára.sk / Journals / Journal of Czech Physicians / 2020 - 7-8

DNA repair in head and neck tumor cells and possibilities of its monitoring to estimate individual tumor radioresistance and selection of optimal primary treatment

Česká verzia

Authors: Zuzana Horáková 1;  MUDr. Jaromír Gumulec 2,3;  Olga Kopečná 4;  Eva Pagáčová 4;  Michal Masařík 2,3;  Alena Bačíková 4;  Iva Falková 4;  Martina Raudenská 2,3;  Tomáš Vičar 5;  Hana Binková 6;  Martin Falk 4
Authors place of work: Klinika otorinolaryngologie a chirurgie hlavy a krku LF UP a FN Olomouc 1;  Ústav patologické fyziologie LF MU v Brně 2;  Fyziologický ústav LF MU v Brně 3;  Oddělení buněčné biologie a radiobiologie, Biofyzikální ústav AV ČR, Brno 4;  Ústav biomedicínského inženýrství, FEKT VUT v Brně 5;  Oddělení ORL, Vojenská nemocnice Brno 6
Published in the journal: Čas. Lék. čes. 2020; 159: 268-274
Category: Review Article

Summary

In order to maximize post-therapeutic quality of life, radio(chemo)therapy becomes preferred over surgery in head-and-neck tumor (HNT) treatment. However, the therapy selection is only based on the clinical experience and patient's preferences as the radiosensitivity markers remain unknown. New possibilities of deciding on the best primary therapy, moving us towards personalized medicine based on quantifiable biomarkers, have been opened by studies on DNA radiation damage and repair in individual patients’ tumors.

Together with the importance of radiotherapy in HNT oncology, we discuss here our preliminary results revealing the existence of several HNT groups with respect to genome stability and repair ability of tumor cells after irradiation. Monitoring of the formation and disappearance of γH2AX/53BP1 foci in tumor cell primo-cultures derived from individual patients suggests that DNA repair capacity of the identified groups correlates with the tumor cell radiosensitivity. Our findings thus improve understanding of HNT biology; nevertheless, the relationship between the repair groups and in vivo response of tumors to radiotherapy must be further studied. Since most HNTs do not suffer from repair defects, although their viability varies after irradiation, pre-therapeutic tests covering the full spectrum of HNT radiosensitivity causes will require the use of a combination of multiple, still undiscovered biomarkers.

Keywords:

Head and neck tumors – primary therapy dilemma – radiotherapy – radioresistance predictive markers – DNA damage and repair

Zdroje
  1. Dušek L, Mužík J, Kubásek M a kol. Webový portál SVOD: Epidemiologie zhoubných nádorů v České republice. Masarykova univerzita, Brno 2005. Dostupné na: www.svod.cz
  2. Falk M, Horáková Z, Svobodová M et al. γH2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity – preliminary methodological study and discussion. EPJD 2017; 71: 241.
  3. Ježková L, Zadneprianetc M, Kulikova E et al. Particles with similar LET values generate DNA breaks of different complexity and reparability: A high-resolution microscopy analysis of γh2AX/53BP1 foci. Nanoscale 2018; 10: 1162–1179.
  4. Štefančíková L, Lacombe S, Salado D et al. Effect of gadolinium-based nanoparticles on nuclear DNA damage and repair in glioblastoma tumor cells. J Nanobiotechnology 2016; 14: 63.
  5. Michaelidesová A, Vachelová J, Puchalská M et al. Relative biological effectiveness in a proton spread-out Bragg peak formed by pencil beam scanning mode. Australas Phys Eng Sci Med 2017; 40: 359–368.
  6. Alsahafi E, Begg K, Amelio I et al. Clinical update on head and neck cancer: molecular biology and ongoing challenges. Cell Death Dis 2019; 10: 540.
  7. Kobayashi K, Hisamatsu K, Suzui N et al. Review of HPV-Related Head and Neck Cancer. J Clin Med 2018; 7: 241.
  8. Rogers SN, Semple C, Babb M, Humphris G. Quality of life considerations in head and neck cancer: United Kingdom National Multidisciplinary Guidelines. J Laryngol Otol 2016; 130(S2): S49–S52.
  9. Golusiński W. Functional organ preservation surgery in head and neck cancer: transoral robotic surgery and beyond. Front Oncol 2019; 9: 293.
  10. Falk M, Falková I. Účinky ionizujícího záření na subcelulární a celulární úrovni, mechanizmy reparace DNA. In: Havránková R (ed.). Klinická radiobiologie. Grada, Praha, 2020: 67–102.
  11. Ganci F, Sacconi A, Manciocco V et al. Radioresistance in head and neck squamous cell carcinoma – possible molecular markers for local recurrence and new putative therapeutic strategies. In: Marcu LG (ed.). Contemporary Issues in Head and Neck Cancer Management. InTech, 2015.
  12. Falk M, Hausmann M, Lukášová E et al. Determining omics spatiotemporal dimensions using exciting new nanoscopy techniques to assess complex cell responses to DNA damage: part A – radiomics. Crit Rev Eukaryot Gene Expr 2014; 24: 205–223.
  13. Falk M, Hausmann M, Lukášová E et al. Determining omics spatiotemporal dimensions using exciting new nanoscopy techniques to assess complex cell responses to DNA damage: part B – structuromics. Crit Rev Eukaryot Gene Expr 2014; 24: 225–247.
  14. Pagáčová E, Štefančíková L, Schmidt-Kaler F et al. Challenges and contradictions of metal nano-particle applications for radio-sensitivity enhancement in cancer therapy. Int J Mol Sci 2019; 20: 588.
  15. Hofer M, Falk M, Komůrková D et al. Two new faces of amifostine: protector from DNA damage in normal cells and inhibitor of DNA repair in cancer cells. J Med Chem 2016; 59: 3003–3017.
  16. Hofer M, Hoferová Z, Falk M. Pharmacological modulation of radiation damage. Does it exist a chance for other substances than hematopoietic growth factors and cytokines? Int J Mol Sci 2017; 18: 1385.
  17. Hofer M, Hoferová Z, Falk M. Brief story on prostaglandins, inhibitors of their synthesis, hematopoiesis, and acute radiation syndrome. Molecules 2019; 24: 4019.
  18. Meyer JL, Kavanagh BD, Purdy JA, Timmerman R (eds.). IMRT, IGRT, SBRT – advances in the treatment planning and delivery of radiotherapy. Front Radiat Ther Oncol 2007; 40.
  19. Gao J, Hu J, Guan X et al. Salvage carbon-ion radiation therapy for locoregionally recurrent head and neck malignancies. Sci Rep 2019; 9: 4259.
  20. Wang H, Jiang H, Van De Gucht M, De Ridder M. Hypoxic radioresistance: can ROS be the key to overcome it? Cancers (Basel) 2019; 11: 112.
  21. Brenner DJ. Proton radiotherapy: the good, the bad, and the uncertain. Oncology (Williston Park) 2012; 26: 168241.
  22. Chen YH, Lu HI, Chien CY et al. Efficacy of different chemotherapy regimens in patients with locally advanced synchronous esophageal and head/neck squamous cell carcinoma receiving curative concurrent chemoradiotherapy. J Clin Med 2020; 9: 197.
  23. Bossi P, Resteghini C, Paielli N et al. Prognostic and predictive value of EGFR in head and neck squamous cell carcinoma. Oncotarget 2016; 7(45): 74362–74379.
  24. Forster MD, Devlin MJ. Immune checkpoint inhibition in head and neck cancer. Front Oncol 2018; 8: 310.
  25. Dandekar M, D’Cruz A. Organ preservation strategies: review of literature and their applicability in developing nations. South Asian J Cancer 2014; 3: 147–150.
  26. Trivedi NP, Swaminathan DK, Thankappan K et al. Comparison of quality of life in advanced laryngeal cancer patients after concurrent chemoradiotherapy vs total laryngectomy. Otolaryngol Head Neck Surg. 2008; 139: 702–707.
  27. Hutcheson KA, Lewin JS. Functional outcomes after chemoradiotherapy of laryngeal and pharyngeal cancers. Curr Oncol Rep. 2012; 14: 158–165.
  28. Tang Z-X, Gong J-L, Wang Y-H et al. Efficacy comparison between primary total laryngectomy and nonsurgical organ-preservation strategies in treatment of advanced stage laryngeal cancer: a meta-analysis. Medicine 2018; 97: e10625.
  29. Binková H, Horáková Z, Tóthová E, Kostřica R. Organ preservation protocol in oropharyngeal cancer. Otorinolaryngol Foniatr 2010; 59: 114–121.
  30. Horáková Z, Binková H, Tóthová E. Results of surgical and nonsurgical treatment in patients with laryngeal cancer. Otorinolaryngol Foniatr 2010; 59: 107–113.
  31. Binková H, Šmardová J, Krpenský A, Kšicová K. Prognóza nádorů hlavy a krku se zaměřením na nádorový supresor p53. Otorinolaryngologie a foniatrie 2004; 53: 189–195.
  32. Jajodia A, Aggarwal D, Chaturvedi AK et al. Value of diffusion MR imaging in differentiation of recurrent head and neck malignancies from post treatment changes. Oral Oncol 2019; 96: 89–96.
  33. de Ridder M, Gouw ZAR, Navran A et al. FDG-PET/CT improves detection of residual disease and reduces the need for examination under anaesthesia in oropharyngeal cancer patients treated with (chemo-)radiation. Eur Arch Otorhinolaryngol. 2019; 276: 1447–1455.
  34. Brierley J, Gospodarowicz MK, Wittekind C et al. TNM: klasifikace zhoubných novotvarů. Grada, Praha, 2020.
  35. Bohlen M, Busch C-J, Sehner S et al. Tumor volume as a predictive parameter in the sequential therapy (induction chemotherapy) of head and neck squamous cell carcinomas. Eur Arch Otorhinolaryngol 2019; 276: 1183–1189.
  36. Gurín D, Slávik M, Shatokhina T et al. Současný pohled na HPV asociované karcinomy orofaryngu a roli p16 jako surogátního markeru high-risk HPV. Klinická onkologie 2019; 32: 252–260.
  37. Tumban EA. Current update on human papillomavirus-associated head and neck cancers. Viruses 2019; 11: 922.
  38. Wolf GT. Options for preserving the larynx in patients with advanced laryngeal and hypopharyngeal cancer. Ear Nose Throat J 2001; 80: 897–901.
  39. Chaput A, Calais J, Robin P et al. Correlation between fluorodeoxyglucose hotspots on pretreatment positron emission tomography/CT and preferential sites of local relapse after chemoradiotherapy for head and neck squamous cell carcinoma. Head Neck 2017; 39: 1155–1165.
  40. Polverini PJ, Lingen MW. A history of innovations in the diagnosis and treatment of oral and head and neck cancer. J Dent Res 2019; 98: 489–497.
  41. Schulz A, Meyer F, Dubrovska A, Borgmann K. Cancer stem cells and radioresistance: DNA repair and beyond. Cancers 2019; 11: 862.
  42. You GR, Cheng AJ, Lee LY et al. Prognostic signature associated with radioresistance in head and neck cancer via transcriptomic and bioinformatic analyses. BMC Cancer 2019; 19: 64.
  43. Raudenská M, Svobodová M, Gumulec J et al. Význam fibroblastů asociovaných s nádorem při patogenezi karcinomů v oblasti hlavy a krku. Klinická onkologie 2020; 33: 39–48.
  44. Nickson CM, Moori P, Carter RJ et al. Misregulation of DNA damage repair pathways in HPV-positive head and neck squamous cell carcinoma contributes to cellular radiosensitivity. Oncotarget 2017; 8: 29963–29975.
  45. Rittich B, Španová A, Falk M et al. Cleavage of double stranded plasmid DNA by lanthanide complexes. J Chromatogr B Analyt Technol Biomed Life Sci 2004; 800: 169–173.
  46. Falk M, Lukášová E, Kozubek S. Higher-order chromatin structure in DSB induction, repair and misrepair. Mutat Res 2010; 704: 88–100.
  47. Svobodová M, Raudenská M, Gumulec J et al. Establishment of oral squamous cell carcinoma cell line and magnetic bead-based isolation and characterization of its CD90/ CD44 subpopulations. Oncotarget 2017; 8: 66254–66269.
  48. Falk M, Falková I, Kopečná O et al. Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants. Sci Rep 2018; 8: 14694.
  49. Bobková E, Depeš D, Lee JH et al. Recruitment of 53BP1 proteins for DNA repair and persistence of repair clusters differ for cell types as detected by single molecule localization microscopy. Int J Mol Sci 2018; 19: 3713.
  50. Foy J-P, Bazire L, Ortiz-Cuaran S et al. A 13-gene expression-based radioresistance score highlights the heterogeneity in the response to radiation therapy across HPV-negative HNSCC molecular subtypes. BMC Medicine 2017; 15: 165.
Štítky
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental Hygienist
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Článok vyšiel v časopise

Journal of Czech Physicians

Číslo 7-8
2020 Číslo 7-8
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