#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Journals / Journal of Czech Physicians / 2020 - 7-8

Biological dosimetry and modern (-omic) methods

Česká verzia

Authors: Gabriela Kultová 1,2;  Marcela Jeličová 1;  Jana Čížková 1;  Zuzana Šinkorová 1;  Aleš Tichý 1
Authors place of work: katedra radiobiologie, Fakulta vojenského zdravotnictví UO, Hradec Králové 1;  katedra biologie, Přírodovědecká fakulta UHK, Hradec Králové 2
Published in the journal: Čas. Lék. čes. 2020; 159: 281-285
Category: Review Article

Summary

The increased risk of acute large-scale radiation exposure of the population underlies the necessity to develop new methods that could provide a rapid assessment of the doses received while using modern high-throughput technologies. At the same time, there is a growing interest in discovering new biomarkers enabling the categorization of irradiated individuals that could be used in epidemiological studies to correlate the estimated absorbed doses with the consequent impact on patient’s health.

The aim of this study was to summarize the current literature on biological dosimetry, specifically ionizing radiation-responsive biomarkers. We briefly describe current knowledge in the field of radiation genomics, metabolomics, and proteomics.

Although the majority of studies that provided a plethora of useful information were conducted in animal models, oncological patients remain the crucial experimental model. The authors describe various biological materials that could be potentially used to predict the effect of ionizing radiation. Plasma proteins appear to be ideal for this purpose. Out of many candidate markers, the ferredoxin reductase (FDXR) seems to be promising, as it has been confirmed in several biodosimetric studies at the level of both human gene and protein.

Keywords:

radiation protection – biomarker – proteomics – Metabolomics – biodosimetry

Zdroje
  1. Byrum SD, Burdine MS, Orr L et al. Time- and radiation-dose dependent changes in the plasma proteome after total body irradiation of non-human primates: implications for biomarker selection. PloS One 2017; 12(3): e0174771.
  2. O’Brien G, Cruz-Garcia L, Majewski M et al. FDXR is a biomarker of radiation exposure in vivo. Sci Rep 2018; 8: 684.
  3. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell 2000; 100: 57–70.
  4. Kannan K, Amariglio N, Rechavi G et al. DNA microarrays identification of primary and secondary target genes regulated by p53. Oncogene 2001; 20: 2225–2234.
  5. Matoušek J, Österreicher J, Linhart P. CBRN – jaderné zbraně a radiologické materiály. SPBI, Ostrava, 2007.
  6. Orth M, Lauber K, Niyazi M et al. Current concepts in clinical radiation oncology. Radiat Environ Biophys 2014; 53: 1–29.
  7. Rieger KE, Hong W-J, Tusher VG et al. Toxicity from radiation therapy associated with abnormal transcriptional responses to DNA damage. Proc Natl Acad Sci USA 2004; 101: 6635–6640.
  8. Reisz JA, Bansal N, Qian J et al. Effects of ionizing radiation on biological molecules – mechanisms of damage and emerging methods of detection. Antioxid Redox Signal 2014; 21: 260–292.
  9. Lu TP, Hsu YY, Lai LC et al. Identification of gene expression biomarkers for predicting radiation exposure. Sci Rep 2014; 4: 6293.
  10. Singh VK, Newman VL, Romaine PL et al. Use of biomarkers for assessing radiation injury and efficacy of countermeasures. Expert Rev Mol Diagn 2016; 16: 65–81.
  11. Amundson SA, Do KT, Shahab S et al. Identification of potential mRNA biomarkers in peripheral blood lymphocytes for human exposure to ionizing radiation. Radiat Res 2000; 154: 342–346.
  12. Oh JH, Wong HP, Wang X, Deasy JO. A bioinformatics filtering strategy for identifying radiation response biomarker candidates. PLoS One 2012; 7: e38870.
  13. Manning G, Kabacik S, Finnon P et al. High and low dose responses of transcriptional biomarkers in ex vivo X-irradiated human blood. Int J Radiat Biol 2013; 89: 512–522.
  14. Budworth H, Snijders AM, Marchetti F et al. DNA Repair and cell cycle biomarkers of radiation exposure and inflammation stress in human blood. PLoS One 2012; 7: e48619.
  15. Ghandhi SA, Smilenov LB, Elliston CD et al. Radiation dose-rate effects on gene expression for human biodosimetry. BMC Med Genomics 2015; 8: 22.
  16. Lacombe J, Sima C, Amundson SA, Zenhausern F. Candidate gene biodosimetry markers of exposure to external ionizing radiation in human blood: a systematic review. PLoS One 2018; 13: e0198851.
  17. Xiao X, Hu M, Liu M, Hu J. 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation. Metabolomics 2016; 6: 1–11.
  18. Cheema AK, Suman S, Kaur P et al. Long-term differential changes in mouse intestinal metabolomics after γ and heavy ion radiation exposure. PLoS One 2014; 9: e87079.
  19. Cheema AK, Mehta KY, Rajagopal MU et al. Metabolomic studies of tissue injury in nonhuman primates exposed to gamma-radiation. Int J Mol Sci 2019; 20: 3360.
  20. Cheema AK, Hinzman CP, Mehta KY et al. Plasma derived exosomal biomarkers of exposure to ionizing radiation in nonhuman primates. Int J Mol Sci 2018; 19: 3427.
  21. Patterson AD, Li H, Eichler GS et al. UPLC-ESI-TOFMS-based metabolomics and gene expression dynamics inspector self-organizing metabolomic maps as tools for understanding the cellular response to ionizing radiation. Anal Chem 2008; 80: 665–674.
  22. Laiakis EC, Hyduke DR, Fornace AJ. Comparison of mouse urinary metabolic profiles after exposure to the inflammatory stressors γ radiation and lipopolysaccharide. Radiat Res 2012; 177: 187–199.
  23. Clish CB. Metabolomics: an emerging but powerful tool for precision medicine. Cold Spring Harb Mol Case Stud 2015; 1: a000588.
  24. Laiakis EC, Mak TD, Anizan S et al. development of a metabolomic radiation signature in urine from patients undergoing total body irradiation. Radiat Res 2014; 181: 350–361.
  25. Chen Z, Coy SL, Pannkuk EL et al. Rapid and high-throughput detection and quantitation of radiation biomarkers in human and nonhuman primates by differential mobility spectrometry-mass spectrometry. J Am Soc Mass Spectrom 2016; 27: 1626–1636.
  26. Soni S, Agrawal P, Kumar N et al. Salivary biochemical markers as potential acute toxicity parameters for acute radiation injury: a study on small experimental animals. Hum Exp Toxicol 2016; 35: 221–228.
  27. Moore HD, Ivey RG, Voytovich UJ et al. The human salivary proteome is radiation responsive. Radiat Res 2014; 181: 521–530.
  28. Swartz HM, Flood AB, Williams BB et al. electron paramagnetic resonance dosimetry for a large-scale radiation incident. Health Phys 2012; 103: 255–267.
  29. Demidenko E, Williams BB, Sucheta A et al. Radiation dose reconstruction from L-band in vivo EPR spectroscopy of intact teeth: comparison of methods. Radiat Meas 2007; 42: 1089–1098.
  30. Tipikin DS, Swarts SG, Sidabras JW et al. possible nature of the radiation-induced signal in nails: high-field EPR, confirming chemical synthesis, and quantum chemical calculations. Radiat Prot Dosimetry 2016; 172: 112–120.
  31. Flood AB, Williams BB, Schreiber W et al. Advances in in vivo EPR tooth biodosimetry: meeting the targets for initial triage following a large-scale radiation event. Radiat Prot Dosimetry 2016; 172: 72–80.
  32. Khan AR, Rana P, Devi MM et al. Nuclear magnetic resonance spectroscopy-based metabonomic investigation of biochemical effects in serum of γ-irradiated mice. Int J Radiat Biol 2011; 87: 91–97.
  33. Lössl P, van de Waterbeemd M, Heck AJ. The diverse and expanding role of mass spectrometry in structural and molecular biology. EMBO J 2016; 35: 2634–2657.
  34. Johnston CJ, Hernady E, Reed C et al. Early alterations in cytokine expression in adult compared to developing lung in mice after radiation exposure. Radiat Res 2010; 173: 522–535.
  35. Bujold K, Hauer-Jensen M, Donini O et al. Citrulline as a biomarker for gastrointestinal-acute radiation syndrome: species differences and experimental condition effects. Radiat Res 2016; 186: 71–78.
  36. Pawar SA, Shao L, Chang J et al. C/EBPδ deficiency sensitizes mice to ionizing radiation-induced hematopoietic and intestinal injury. PLoS One 2014; 9: e94967.
  37. Jones JW, Tudor G, Bennett A et al. Development and validation of a LC-MS/MS assay for quantitation of plasma citrulline for application to animal models of the acute radiation syndrome across multiple species. Anal Bioanal Chem 2014; 406: 4663–4675.
  38. Ossetrova NI, Sandgren DJ, Blakely WF. Protein biomarkers for enhancement of radiation dose and injury assessment in nonhuman primate total-body irradiation model. Radiat Prot Dosimetry 2014; 159: 61–76.
  39. Yi L, Mu H, Hu N et al. Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings. Biosci Rep 2018; 38: BSR20180536.
  40. Lee Y, Pujol Canadell M, Shuryak I et al. Candidate protein markers for radiation biodosimetry in the hematopoietically humanized mouse model. Sci Rep 2018; 8: 13557.
  41. Pannkuk EL, Fornace AJ, Laiakis EC. Metabolomic applications in radiation biodosimetry: exploring radiation effects through small molecules. Int J Radiat Biol 2017; 93: 1151–1176.
  42. Marchetti F, Coleman MA, Jones IM, Wyrobek AJ. Candidate protein biodosimeters of human exposure to ionizing radiation. Int J Radiat Biol 2006; 82: 605–639.
  43. Turner HC, Brenner DJ, Chen Y et al. Adapting the γ-H2AX assay for automated processing in human lymphocytes. 1. Technological Aspects. Radiat Res 2011; 175: 282–290.
  44. Turner HC, Shuryak I, Weber W et al. γ-H2AX kinetic profile in mouse lymphocytes exposed to the internal emitters cesium-137 and strontium-90. PLoS One 2015; 10: e0143815.
  45. Amundson SA, Bittner M, Meltzer P et al. Biological indicators for the identification of ionizing radiation exposure in humans. Expert Rev Mol Diagn 2001; 1: 211–219.
  46. Kim D, Marchetti F, Chen Z et al. Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation. Sci Rep 2013; 3: 2234.
  47. Schauer DA, Desrosiers MF, Le FG et al. EPR dosimetry of cortical bone and tooth enamel irradiated with X and gamma rays: study of energy dependence. Radiat Res 1994; 138: 1–8.
  48. Paul S, Barker CA, Turner HC et al. prediction of in vivo radiation dose status in radiotherapy patients using ex vivo and in vivo gene expression signatures. Radiat Res 2011; 175: 257–265.
  49. Blakely WF, Ossetrova NI, Whitnall MH et al. Multiple parameter radiation injury assessment using a nonhuman primate radiation model-biodosimetry applications. Health Phys 2010; 98: 153–159.
Štítky
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental Hygienist
Článek Crucial moments in Czechoslovak radiobiology history
Článek Biological effects of ionizing radiation
Článek Modern trends in radiotherapy
Článek DNA repair in head and neck tumor cells and possibilities of its monitoring to estimate individual tumor radioresistance and selection of optimal primary treatment
Článek Trends in pharmacological stimulation of regeneration of ionizing radiation-damaged mammalian organism
Článek Úvodem
Článek Editorial
Článek Advances in research of DNA damage and repair in cells exposed to various types of ionizing radiation in the era of super-resolution optical microscopy
Článek Principles and new perspectives in the vaccination against SARS-CoV-2 virus
Článek Age is not a disease: Evolution of protective antibodies against SARS-CoV-2 in seniors from the Břevnice nursing home
Článek Season of the year, vitamin D and COVID-19
Článok vyšiel v časopise

Journal of Czech Physicians

Číslo 7-8
2020 Číslo 7-8
Stáhnout celé číslo v PDF
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#