Changes in pulmonary fibroblasts respiration in vitro after repeated short-term hyperoxic exposure

Česká verzia

Authors: J. Dejmek ^1,2; M. Marková ^2,3; M. Kripnerová ⁴; M. Čedíková ^2,3; Z. Tůma ²; V. Babuška ⁵; L. Bolek ^1,2; J. Kuncová ^1,2,3
Authors place of work: Ústav biofyziky, Lékařská fakulta v Plzni, Univerzita Karlova, Plzeň, vedoucí doc. MUDr. Jitka Kuncová, Ph. D. ¹; Biomedicínské centrum, Lékařská fakulta v Plzni, Univerzita Karlova, Plzeň, vědecký ředitel doc. MUDr. Milan Štengl, Ph. D. ²; Ústav fyziologie, Lékařská fakulta v Plzni, Univerzita Karlova, Plzeň, vedoucí doc. MUDr. Milan Štengl, Ph. D. ³; Ústav biologie, Lékařská fakulta v Plzni, Univerzita Karlova, Plzeň, vedoucí doc. RNDr. Martin Pešta, Ph. D. ⁴; Ústav lékařské chemie a biochemie, Lékařská fakulta v Plzni, Univerzita Karlova, Plzeň, vedoucí prof. MUDr. Jaroslav Racek, DrSc. ⁵
Published in the journal: Pracov. Lék., 70, 2018, No. 3-4, s. 130-135.
Category:

Summary

Study aims to evaluate effects of repeated exposure to HBO on mitochondrial respiration assessed by high-resolution respirometry (HRR), cell viability estimated by PrestoBlue® reaction, morphology analyzed by routine phase contrast and fluorescent microscopy, and superoxide dismutase (SOD) and citrate synthase (CS) activities using human lung fibroblasts. The cells were exposed to HBO (3 ATA) for 2 hours per day for 5 consecutive days. One day after the last exposure, HBO cells displayed significantly smaller area and perimeter, compromised viability and elevated SOD activity. No changes were detected in CS activity or quality of mitochondrial network. HRR revealed impaired mitochondrial oxygen consumption manifested by increased leak respiration, decreased activity of complex II and compromised ATP-related oxygen consumption when fatty acids were oxidized. Our findings document that in conditions mimicking chronic intermittent exposure to HBO, lung fibroblasts suffer from compromised mitochondrial respiration linked to complex II and impaired cellular growth in spite of increased antioxidant defense. Underlying mechanism of this HBO-induced mitochondrial dysfunction should be further explored.

Keywords:

hyperbaric hyperoxia – high resolution respirometry – human fetal lung fibroblasts – mitochondria – superoxide dismutase

Zdroje

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