The Influence of Laser Irradiation with Different Power Densities on Incisional Wound Healing in Healthy and Diabetic Rats

Česká verzia

Authors: R. Kilík ¹; J. Bober ¹; P. Gál ²; B. Vidinský ²; M. Mokrý ²; F. Longauer ³; J. Sabo ²
Authors place of work: l. chirurgická klinika, Lekárska fakulta Univerzity Pavla Jozefa Šafárika v Košiciach, Slovenská republika, prednosta kliniky: prof. MUDr. J. Bober, CSc. ¹; Ústav lekárskej biofyziky, Lekárska fakulta Univerzity Pavla Jozefa Šafárika v Košiciach, Slovenská republika, prednosta ústavu: doc. RNDr. J. Sabo, CSc, mim. prof. ²; Ústav súdneho lekárstva. Lekárska fakulta Univerzity Pavla Jozefa Šafárika v Košiciach, Slovenská republika, prednosta ústavu: MUDr. N. Bobrov, CSc. ³
Published in the journal: Rozhl. Chir., 2007, roč. 86, č. 7, s. 384-387.
Category: Monothematic special - Original

Summary

Introduction:
The optimal parameters of low level laser therapy (LLLT) are still under debate. It has been documented that a dose or 5 J/cm² would be capable to accelerate the wound healing process in patients. However, the optimal delivering form, i.e. power intensity, is unknown. Therefore, the aim of our study was to compare different power densities of LLLT.

Materials and Methods:
Sixteen male Sprague-Dawley rats were included in this experiment and randomized into two groups, normal healthy group and streptozotocine induced diabetic group. In general anesthesia four full thickness skin incisions were performed under standard aseptic conditions on the back of each rat and immediately closed using intradermal running suture. Three wounds were stimulated with diode laser (wavelength: 635 nm; daily dose 5 J/cm²; power densities: 1 mW/cm², 5 mW/cm² and 15 mW/cm²) each with different power density while the fourth wound served as control. Six days after surgery animals were sacrificed and samples removed for histological evaluation.

Results:
Our study demonstrated that LLLT positively influences wound healing. The most significant changes were observed in wounds stimulated at the highest power density 15 mW/cm². Since using the highest power density the shortest time is needed to achieve the optimal daily dose of 5 J/cm², it can be suggested that 15 mW/cm² might be optimal parameter for such a therapy in patients.

Key words:
wound healing – LLLT – diabetes mellitus – diode laser

Zdroje

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