Experimental Tumor Therapy Using Intratumoral Injection of Dendritic Cells

Česká verzia

Authors: A. Kučera; R. Škába; R. Spíšek ¹; P. Pajer ²; M. Červinková ³
Authors place of work: Klinika dětské chirurgie, UK 2. LF, Fakultní nemocnice Motol, přednosta: prof. MUDr. J. Šnajdauf, DrSc. ; Ústav imunologie, UK 2. LF, Fakultní nemocnice Motol, přednosta: prof. MUDr. J. Bartůňková, DrSc. ¹; Oddělení molekulární virologie, Ústav molekulární genetiky AV ČR, RNDr. M. Dvořák, CSc. ²; Kardiochirurgická klinika FNKV, přednosta: prof. MUDr. Z. Straka, CSc. ³
Published in the journal: Rozhl. Chir., 2009, roč. 88, č. 7, s. 368-372.
Category: Monothematic special - Original

Summary

Aim of study:
After the treatment of tumors, we often encounter a minimal residual sickness. However, the elimination of these leftover tumor cells is crucial for the patient. In the past years one of the most discussed options for this treatment is Imunotherapy, mainly by Dendritic cells. Dendritic cells are the most efficient cells out of the antigen presenting cell group.

Methods and results:
In the first part of the project, we perfected a technique of inducting a tumor on an experimental model. We inducted the tumor by the use of Carcinogenic substances or with the help of the Sarkom line imortalized fibroblasts. Another important part of the project was perfecting the method for the preparation of undeveloped dendritic cells from periphery blood monocytes. After these significant procedures were developed and perfected we moved onto the main part of the study. The Induction of a tumor by the carcenogenic substances Ethylennitrosamin and Phenobarbital was successful only in 20 % of the cases and therefore, was unusable for our experiment. We inducted the tumors with the Sarkom line method. After the application of dendritic cells into the tumor, a decrease in the development of the growth of the tumor was achieved.

Conclusion:
Imunotherapy using dendritic cells as a basis for treatment is a perspective method for treatment of tumors.

Key words:
dendritic cells – surgery – tumor induction

Zdroje

1. Fučíková, J., Kučera, A., Bartůňková, J., Špíšek, R. Role imunitního systému v obraně proti nádorům a strategie protinádorové imunoterapie, Alergie, 2008, 2, str. 119.

2. Banchereau, J., Briere, F., Caux, C., et al. Immunobiology of dendritic cells. Annu. Rev. Immunol., 2000, 18, s. 767–811.

3. Dhodapkar, M. V., Steinman, R. M., Krasovsky, J., Munz, C., Bharwaj, N. Antigen-specific inhibition of effector T cell function in humans after injection of immature dendritic cells. J. Exp. Med., 2001, 193(2), s. 233–238.

4. Spíšek, R., Bražová, J., Rožková, D., Zapletalová, K., Šedivá, A., Bartůňková, J.: Maturation of dendritic cells by bacterial immunomodulators. Vaccine, 2004, 22(21-22), s. 2761–2768.

5. Spíšek, R., Bougras, G., Ebstein, F., et al. Transient exposure of dendritic cells to maturation stimuli is sufficient to induce complete phenotypic maturation while preserving their capacity to respond to subsequent restimulation. Cancer Immunol. Immunother., 2003, 52(7), s. 445–454.

6. Meenakshi, V., Hideki, W., Nobuyasu, T., Yoshihisa, Y., Shuzo, O., Shinji, Y., Shoji, F. Inhibitory Effects of S-Methylcysteine and Cysteine on the Promoting Potential of Sodium Phenobarbital on Rat Liver Carcinogenesis. Jpn. J. Cancer Res., 2000, 91, 780–785.

7. Barbisan, L. F., Miyamoto, M, Scholastici, C, Salvadori, D. M. F., Ribeiro, L. R., Eira, A. F., Camargo, J. Influence of aqueous extract of Agaricus blazei on rat liver toxicity induced by different doses of diethylnitrosamine. Journal of Ethnopharmacology, 2002, 83, 25–32.

8. Rocha, N., Barbisan, L., Oliveira, M., Camargo, J. Effects of Fasting and Intermittent Fasting on rat Hepatocarcinogenesis Induced by Diethylnitrosamine. Teratogenesis, Carcinogenesis, and Mutagenesis, 2002, 22, 129–138.

9. Kučera, A., Šnajdauf, J., Vyhnánek, M, Morávek, J., Kodet, R., Stejskalová, E., Dvořáková, M. Lipoblastoma in Children: an Analysis of 5 Cases. Acta Chir. Belg., 2008, 108, 580–582.

10. Mognato, G., Cecchetto, G., Carlim, M. Is surgical treatment of lipo-blastoma always necessary? J. Pediatr. Surg., 2000, 35: 1511–1513.

11. Spíšek, R., Bartůňková, J. Dendritická buňka v protinádorové imunitě. Vesmír, 2003, 82(5), s. 254–256.