Plasmatic Levels of Proinflammatory Cytokines in Abdominal Aortic Aneurysms

Česká verzia

Authors: V. Třeška ¹; O. Topolčan ²; J. Kočová ³; J. Moláček ¹; K. Houdek ¹; Z. Tonar ³; J. Vrzalová ²; Inka Třešková ¹ ; V. Křížková ³; L. Boudová ³
Authors place of work: Chirurgická klinika Lékařské fakulty a Fakultní nemocnice v Plzni, přednosta: prof. MUDr. Vladislav Třeška, DrSc. ¹; Oddělení imunochemické diagnostiky Lékařské fakulty a Fakultní nemocnice v Plzni, přednosta: prof. MUDr. Ondřej Topolčan, CSc. ²; Ústav histologie a embryologie Lékařské fakulty UK v Plzni, přednostka: doc. MUDr. Jitka Kočová, CSc. ³
Published in the journal: Rozhl. Chir., 2011, roč. 90, č. 1, s. 37-41.
Category: Monothematic special - Original

Summary

Introduction:
Inflammation within the abdominal aortic wall is generally considered a very significant ethiopathogenic factor in the development of abdominal aortic aneurysms. Proinflammatory cytokines are important mediators of inflammation within the abdominal aortic wall.

Aim:
The aim of the study was to research, whether plasmatic levels of certain proinflammatory cytikens, which can commonly be evaluated (TNFα, IL-1, -2, -6 a -8), play a significant role in the development of AAA.

Method:
The prospective non- randomized study included 345 patients with AAAs. The patients were assigned to 5 subgroups based on their symptoms and AAA diameters. The first subgroup included patients with symptomatic AAAs, including AAA ruptures (N = 69), the second subgroup included subjects with asymptomatic AAAs (N = 276) with AAA diameters up to 5 cm (N = 72), the third subgroup included 5 cm (N = 72), the fourth included 5–8 cm (N = 192) and the fifth subgroup included subjects with AAA diameters of more than 8 cm (N = 81). The mean age of patients was 74.1 ± 7.8 years (56–84 y.o.a.). The male to female ratio was 5:1. The control group included 30 healthy volunteer subjects of similar age and male to female rates, who had no clinical signs of arterial disorders. Plasmatic levels of cytokines were evaluated from venous blood samples using ELISA (Bender, Austria) testing. Statistical assessment of the results was performed using ANOVA and Wilcoxon tests with Spearman’s correlation. P values< 0.05 were considered significant.

Results:
Plasmatic concentrations of proinflammatory cytokines were found to be statistically significantly higher in patients with AAAs compared to those in healthy volunteers. Plasmatic IL8 levels were significantly decreasing proportionally to decreasing AAA diameters (p < 0.05). TNFα levels were found to be significantly low in symptomatic patients with AAA ruptures (p < 0.05).

Conclusion:
The study confirmed the significance of proinflammatory cytokines levels monitoring in AAA patients. The authors showed that, for instance IL8 activity and to a certain extent TNFα activity, is the highest in small and developing AAAs. These findings would be significant for customized medication therapy aimed at blocking the effects of these factors on the inflammatory process within the AAA wall.

Key words:
abdominal aortic aneurysm– proinflammatory cytokines

Zdroje

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