Confocal laser endomicroscopy in the diagnostics of gastrointestinal lesions − literary review and personal experience
Authors:
M. Kollár 1; J. Krajčíová 2; R. Husťak 3,4; J. Malušková 1; M. Kment 1; Z. Vacková 2; J. Špičák 2; J. Martínek 2,3
Authors place of work:
Pracoviště klinické a transplantační patologie, Institut klinické a experimentální medicíny, Praha
1; Klinika hepatogastroenterologie, Institut klinické a experimentální medicíny, Praha
2; Interná klinika, Fakultná nemocnica Trnava
3; Katedra laboratórnych vyšetrovacích metód v zdravotníctve, Trnavská univerzita
4
Published in the journal:
Rozhl. Chir., 2018, roč. 97, č. 12, s. 531-538.
Category:
Review
Summary
Introduction:
Confocal laser endomicroscopy (CLE) is a new method enabling real-time histopathological diagnosis. Two platforms of CLE are used standardly: the so called probe-based CLE in which an endomicroscopic probe is inserted through an endoscope, and so-called needle-based CLE inserted through a needle.
Methods:
CLE enables evaluation of epithelial and subepithelial structures with 1000x magnification in any part of the gastrointestinal system. The main advantage over conventional biopsies can be immediate diagnosis and larger area evaluated. CLE might play a promising role in indeterminate biliary lesions where biopsies are often not sufficient and fail to allow for accurate diagnosis. CLE can also have a promising potential in pancreatic lesions where it is used along with the needle designed for tissue aspiration during endosonography. It is also possible to use CLE in other organ systems and medical specialties, e.g. in pneumology, neurosurgery, ENT and others.
Conclusion:
CLE is a promising diagnostic method; however, it is unlikely to replace standard biopsies currently since these are still considered a gold standard of histopathological diagnosis. High purchase price is a main disadvantage of the method, hampering its expansion in medicine.
Key words:
confocal laser endomicroscopy − CLE − biliary strictures − pancreatic lesions − esophageal carcinoma
Rozhl Chir 2018;97:531–538
Zdroje
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