Studium mechanických vlastností s využitím mikroskopie atomárních sil
Authors:
J. Malohlava; K. Tománková; P. Kolář; H. Kolářová
Authors place of work:
Ústav lékařské biofyziky, Lékařská fakulta, Univerzita Palackého v Olomouci, Česká republika
Published in the journal:
Lékař a technika - Clinician and Technology No. 3, 2013, 43, 5-9
Category:
Review
Summary
Mikroskopie atomárních sil (AFM) se řadí mezi moderní zobrazovací techniky, které poskytují obraz v subnanometrickém rozlišení. Na rozdíl od ostatních technik ji lze použít i v kapalinách, které imitují přirozené prostředí, což je obzvlášť výhodné při studiu biologických vzorků. Od roku 1986 se vyvinula v univerzální nástroj, který kromě výškového zobrazení poskytuje mapy elastických a viskoelastických vlastností. Naše práce má za úkol představit nové techniky zabývající se studiem mechanických vlastností buněk – Peak Force Tapping a Stiffness tomography. Oba přístupy reprezentují jedinečný způsob práce se silovými křivkami, které jsou základem pro určení mechanických vlastností.
Klíčová slova:
mikroskopie atomárních sil, mechanické vlastnosti, Peak Force Tapping, Stiffness tomography
Zdroje
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Štítky
BiomedicineČlánok vyšiel v časopise
The Clinician and Technology Journal
2013 Číslo 3
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