Real time RT-PCR panel pro identifikaci dvanácti virů hemoragických horeček

Úvod:
Virové hemoragické horečky jsou způsobovány zástupci čtyř virových čeledí a patří mezi choroby s velmi vysokou smrtností. Pro jejich diagnostiku není dostupný žádný komerční kit.

Výsledky:
Vytvořili jsme real time RT-PCR panel pro detekci a identifikaci virů Ebola, Marburg, Lassa, Guanarito, Machupo, Sabiá, Seoul, Puumala, Hantaan, virus krymsko-konžské hemoragické horečky a horečky Rift Valley. Protokol funguje na principu jednotných reakčních podmínek a byl testován na různých PCR přístrojích jak kapilárních, tak i destičkových včetně polní verze termocykleru Ruggedized Advanced Pathogen Identification Device (R.A.P.I.D.) (Idaho Technology, Inc.).

Závěr:
V kombinaci s adaptovanými protokoly z již publikovaných prací představujeme jednoduchý detekční systém pro rychlou identifikaci původců virových hemoragických horeček z čeledí filoviru, arenavirů a bunyavirů s dostatečnou senzitivitou a specificitou pro použití laboratořích prvního kontaktu a pro diagnostiku v polních podmínkách.

Klíčová slova:
hemoragické horečky – filoviry – arenaviry – real time RT-PCR –diagnostika

Autoři: M. Fajfr ^1,2; V. Neubauerová ³; P. Pajer ³; P. Kubíčková ³; D. Růžek ^4,5
Působiště autorů: Faculty of Military Health Sciences, University of Defense, Hradec Králové ¹; Institute of Clinical Microbiology, University Hospital in Hradec Králové, Hradec Králové ²; Central Military Institute of Health, Army of the Czech Republic, Prague ³; Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice ⁴; Department of Virology, Veterinary Research Institute, Brno ⁵
Vyšlo v časopise: Epidemiol. Mikrobiol. Imunol. 63, 2014, č. 3, s. 238-244
Kategorie: Souhrnná sdělení, původní práce, kazuistiky

Souhrn

Klíčová slova:
hemoragické horečky – filoviry – arenaviry – real time RT-PCR –diagnostika

Zdroje

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