Vplyv frakcionovaného ožiarenia na hipokampus v experimentálnom modeli

Východiska:
Ionizujúce žiarenie ovplyvňuje tkanivovú homeostázu a môže viesť k jeho morfologickému a funkčnému poškodeniu. Cieľom štúdie bolo skúmať krátkodobé a dlhodobé účinky ionizujúceho žiarenia na populáciu buniek osidľujúcu hipokampus dospelého potkana.

Materiál a metódy:
Dospelým samcom potkanov kmeňa Wistar sme ožiarili cranium frakcionovanou dávkou gama žiarenia (celková dávka bola 20 Gy) a vyšetrovali 30 a 100 dní po expozícii. Pomocou histochemickej metodiky Fluoro-Jade C na dôkaz degenerujúcich neurónov, imunohistochemického farbenia na detekciu astrocytov a konfokálnej mikroskopie sme kvantitatívne hodnotili neurodegeneratívne zmeny v gyrus dentatus a oblasti CA1 hipokampu.

Výsledky:
V obidvoch vyšetrovaných oblastiach sme zistili signifikantný nárast počtu Fluoro-Jade C značených neurónov, predovšetkým v skupine prežívajúcej 30 dní po ožiarení. Počet GFAP-imunoreaktívnych astrocytov sa počas celého experimentu znížil len nepatrne.

Záver:
Naše súčasné výsledky poukazujú na to, že postradiačná odpoveď populácie buniek, ktorá tvorí hipokampus môže zohrávať úlohu vo vývoji neskorých postradiačných prejavov, ktoré sú z hľadiska prognózy veľmi nepriaznivé.

Kľúčové slová:
ionizujúce žiarenie – dávka žiarenia – potkan – hipokampus – Fluoro-Jade C – GFAP

Práca bola financovaná z projektu Centrum translačnej medicíny/Vytvorenie nového diagnostického algoritmu pri vybraných nádorových ochoreniach, ITMS: 26220220021 spolufinancovanými zo zdrojov EÚ a Európskeho fondu regionálneho rozvoja.

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Obdržané:
8. 3. 2015

Prijaté:
5. 4. 2015

Autoři: S. Bálentová ¹; E. Hajtmanová ²; B. Filova ³; V. Borbelyova ⁴; J. Lehotský ⁵
Působiště autorů: Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovak Republic ¹; Department of Radiotherapy and Oncology, Martin University Hospital, Martin, Slovak Republic ²; Institute of Medical Physics, Biophysics, Informatics and Telemedicine, Faculty of Medicine, Comenius University in Bratislava, Slovak Republic ³; Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Slovak Republic ⁴; Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovak Republic ⁵
Vyšlo v časopise: Klin Onkol 2015; 28(3): 191-199
Kategorie: Původní práce
prolekare.web.journal.doi_sk: https://doi.org/10.14735/amko2015191

Souhrn

Obdržané:
8. 3. 2015

Prijaté:
5. 4. 2015

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