A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs

English version

Autoři: In-Cheol Cho ^aff001; Hee-Bok Park ^aff002; Jin Seop Ahn ^aff003; Sang-Hyun Han ^aff004; Jae-Bong Lee ^aff005; Hyun-Tae Lim ^aff006; Chae-Kyoung Yoo ^aff007; Eun-Ji Jung ^aff008; Dong-Hwan Kim ^aff003; Wu-Sheng Sun ^aff003; Yuliaxis Ramayo-Caldas ^aff010; Sang-Geum Kim ^aff001; Yong-Jun Kang ^aff001; Yoo-Kyung Kim ^aff004; Hyun-Sook Shin ^aff001; Pil-Nam Seong ^aff001; In-Sul Hwang ^aff012; Beom-Young Park ^aff012; Seongsoo Hwang ^aff012; Sung-Soo Lee ^aff013; Youn-Chul Ryu ^aff014; Jun-Heon Lee ^aff015; Moon-Suck Ko ^aff001; Kichoon Lee ^aff016; Göran Andersson ^aff017; Miguel Pérez-Enciso ^aff018; Jeong-Woong Lee ^aff003
Působiště autorů: National Institute of Animal Science, Rural Development Administration, Jeju, Republic of Korea ^aff001; Department of Animal Resources Science, College of Industrial Sciences, Kongju National University, Yesan, Republic of Korea ^aff002; Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ^aff003; Educational Science Research Institute, Jeju National University, Jeju, Republic of Korea ^aff004; Korea Zoonosis Research Institute, Chonbuk National University, Iksan, Republic of Korea ^aff005; Department of Animal Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea ^aff006; Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea ^aff007; Bio-Medical Science Co., Ltd., Gimpo, Republic of Korea ^aff008; Department of Functional Genomics, University of Science and Technology, Daejeon, Republic of Korea ^aff009; Génétique Animale et Biologie Intégrative (GABI), INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France ^aff010; Animal Breeding and Genetics Program, Institute for Research and Technology in Food and Agriculture (IRTA), Torre Marimon, Caldes de Montbui, Spain ^aff011; National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea ^aff012; National Institute of Animal Science, Rural Development Administration, Namwon, Republic of Korea ^aff013; Division of Biotechnology, SARI, Jeju National University, Jeju, Republic of Korea ^aff014; Division of Animal and Dairy Science, Chungnam National University, Deajeon, Republic of Korea ^aff015; Department of Animal Sciences, College of Food, Agricultural, and Environmental Sciences, The Ohio State University, Columbus, OH, United States of America ^aff016; Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden ^aff017; Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB Consortium, Barcelona, Spain ^aff018; Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Barcelona, Spain ^aff019; ICREA, Carrer de Lluís Companys, Barcelona, Spain ^aff020
Vyšlo v časopise: A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. PLoS Genet 15(10): e32767. doi:10.1371/journal.pgen.1008279
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1008279

Souhrn

Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5′-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.

Klíčová slova:

Sequence motif analysis – Genome-wide association studies – Meat – Fibroblasts – Skeletal muscles – Variant genotypes – Luciferase – Swine

Zdroje

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