Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (, Hymenoptera: Apidae)
The importance of honey bees to the world economy far surpasses their contribution in terms of honey production; they are responsible for up to 30% of the world's food production through pollination of crops. Since fall 2006, honey bees in the U.S. have faced a serious population decline, due in part to a phenomenon called Colony Collapse Disorder (CCD), which is a disease syndrome that is likely caused by several factors. Data from an initial study in which investigators compared pathogens in honey bees affected by CCD suggested a putative role for Israeli Acute Paralysis Virus, IAPV. This is a single stranded RNA virus with no DNA stage placed taxonomically within the family Dicistroviridae. Although subsequent studies have failed to find IAPV in all CCD diagnosed colonies, IAPV has been shown to cause honey bee mortality. RNA interference technology (RNAi) has been used successfully to silence endogenous insect (including honey bee) genes both by injection and feeding. Moreover, RNAi was shown to prevent bees from succumbing to infection from IAPV under laboratory conditions. In the current study IAPV specific homologous dsRNA was used in the field, under natural beekeeping conditions in order to prevent mortality and improve the overall health of bees infected with IAPV. This controlled study included a total of 160 honey bee hives in two discrete climates, seasons and geographical locations (Florida and Pennsylvania). To our knowledge, this is the first successful large-scale real world use of RNAi for disease control.
Vyšlo v časopise:
Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (, Hymenoptera: Apidae). PLoS Pathog 6(12): e32767. doi:10.1371/journal.ppat.1001160
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1001160
Souhrn
The importance of honey bees to the world economy far surpasses their contribution in terms of honey production; they are responsible for up to 30% of the world's food production through pollination of crops. Since fall 2006, honey bees in the U.S. have faced a serious population decline, due in part to a phenomenon called Colony Collapse Disorder (CCD), which is a disease syndrome that is likely caused by several factors. Data from an initial study in which investigators compared pathogens in honey bees affected by CCD suggested a putative role for Israeli Acute Paralysis Virus, IAPV. This is a single stranded RNA virus with no DNA stage placed taxonomically within the family Dicistroviridae. Although subsequent studies have failed to find IAPV in all CCD diagnosed colonies, IAPV has been shown to cause honey bee mortality. RNA interference technology (RNAi) has been used successfully to silence endogenous insect (including honey bee) genes both by injection and feeding. Moreover, RNAi was shown to prevent bees from succumbing to infection from IAPV under laboratory conditions. In the current study IAPV specific homologous dsRNA was used in the field, under natural beekeeping conditions in order to prevent mortality and improve the overall health of bees infected with IAPV. This controlled study included a total of 160 honey bee hives in two discrete climates, seasons and geographical locations (Florida and Pennsylvania). To our knowledge, this is the first successful large-scale real world use of RNAi for disease control.
Zdroje
1. vanEngelsdorpD
MeixnerMD
2010 A historical review of managed honey bee populations in Europe and the United States and the factors that may affect them. J Invertebr Pathol 103 Suppl 1 S80 95
2. KleinAM
VaissièreBE
CaneJH
Steffan-DewenterI
CunninghamSA
2007 Importance of pollinators in changing landscapes for world crops. Proc Royal Soc London Series B, Biol Sci 274 303 313
3. GallaiN
SallesJ-M
SetteleJ
VaissièreBE
2009 Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecol Econ 68 810 821
4. NeumannP
CarreckNL
2010 Honey bee colony losses. J Apic Res 49 1 6
5. vanEngelsdorpD
UnderwoodR
CaronD
HayesJJr
2007 An estimate of managed colony losses in the winter of 2006–2007: A report commissioned by the Apiary Inspectors of America. Amer Bee Jour 147 599 603
6. Cox-FosterDL
ConlanS
HolmesEC
PalaciosG
EvansJD
2007 A metagenomic survey of microbes in honey bee colony collapse disorder. Science 318 283 287
7. vanEngelsdorpD
EvansJD
SaegermanC
MullinC
HaubrugeE
2009 Colony Collapse Disorder: A Descriptive Study. PloS ONE 4 e6481
8. JohnsonRM
EvansJD
RobinsonGE
BerenbaumMR
2009 Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera). Proc Natl Acad Sci USA 106 14790 14795
9. EllisJD
MunnPA
2005 The worldwide health status of honey bees. Bee World 86 88 101
10. HighfieldAC
El NagarA
MackinderLC
NoelLM
HallMJ
2009 Deformed wing virus implicated in overwintering honeybee colony losses. Appl Environ Microbiol 75 7212 7220
11. MaoriE
PaldiN
ShafirS
KalevH
TsurE
2009 IAPV, a bee-affecting virus associated with Colony Collapse Disorder can be silenced by dsRNA ingestion. Ins Mol Biol 18 55 60
12. PriceDR
GatehouseJA
2008 RNAi-mediated crop protection against insects. Trends Biotech 26 393 400
13. MathieuO
BenderJ
2004 RNA-directed DNA methylation. J Cell Sci 117 4881 4888
14. MatzkeM
AufsatzW
KannoT
DaxingerL
PappI
2004 Genetic analysis of RNA-mediated transcriptional gene silencing. Biochim Biophys Acta 1677 129 141
15. BrodersenP
VoinnetO
2006 The diversity of RNA silencing pathways in plants. Trends Genet 22 268 280
16. ZaratieguiM
IrvineDV
MartienssenRA
2007 Noncoding RNAs and gene silencing. Cell 128 763 776
17. SiomiH
SiomiM
2009 On the road to reading the RNA-interference code. Nature 457 396 404
18. The Honey Bee Consortium 2006 Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443 931 949
19. AronsteinK
PankiwT
SaldivarE
2006 SID-I is implicated in systemic gene silencing in the honey bee. J Apiculture Res Bee World 45 20 24
20. LipardiC
PatersonBM
2009 Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression. PNAS 106 15645 15650
21. FengQ
MorrisTJ
2005 Suppressors of RNA silencing encoded by plant viruses and their role in viral infections. FEBS Letters 579 5958 5964
22. MaoriE
LaviS
Mozes-KochR
GantmanY
PeretzY
2007 Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination. J Gen Virol 88 3428 3438
23. Cox-FosterD
vanEngelsdorpD
2009 Solving the mystery of the disappearing bees. Sci Amer 300 40 47
24. vanEngelsdorpD
HayesJJr
UnderwoodRM
PettisJ
2008 A survey of honey bee colony losses in the US, fall 2007 to spring 2008. PLoS One 3 e4071
25. ChenYP
PettisJS
CollinsA
FeldlauferMF
2006 Prevalence and transmission of honeybee viruses. Appl Environ Microbiol 72 606 611
26. LiuX
ZhangY
YanX
HanR
2010 Prevention of Chinese sacbrood virus infection in Apis ceranae using RNA interference. Curr Microbiol (online) DOI 10.1007/s00284-010-9633-2
27. RosenkranzP
AumeierP
ZiegelmannB
2010 Biology and control of Varroa destructor. J Invertebr Pathol 103 Suppl 1 S96 119
28. Bowen-WalkerPL
MartinSJ
GunnA
1999 The transmission of deformed wing virus between honeybees (Apis mellifera L.) by the ectoparasitic mite Varroa jacobsoni Oud. J Invertebr Pathol 73 101 106
29. HigesM
Martin-HernandezR
BotiasC
BailonEG
Gonzalez-PortoAV
2008 How natural infection by Nosema ceranae causes honeybee colony collapse. Environ Microbiol 10 2659 2669
30. ChenYP
EvansJD
SmithIB
PettisJS
2008 Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. J Invertebr Pathol 97 186 188
31. PaldiN
GlickE
OlivaM
ZilberbergY
AubinL
PettisJS
ChenYP
EvansJD
2010 Effective gene silencing of a microsporidian parasite associated with honey bee (Apis mellifera) colony declines. Appl Environ Microbiol 76 5960 5964
32. BurgettM
BurikamI
1985 Number of adult honey bees (Hymenoptera: Apidae) occupying a comb: a standard for estimating colony populations. J Econ Entomol 78 1154 1156
33. Stewart-OatenA
MurdochWW
ParkerKR
1986 Environmental impact assessment: “Pseudoreplication” in Time? Ecology 67 929 940
34. SmithEP
2002 BACI design.
El-ShaarawiAH
PiegorschWW
Encyclopedia of Environmetrics New York, , New York, USA John Wiley and Sons 141 148
35. Institute SAS 2004 SAS computer program, version 9.1.3 By Institute, S, Carry, NC
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Hygiena a epidemiológia Infekčné lekárstvo LaboratóriumČlánok vyšiel v časopise
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