Determination of phthalates and bisphenol A and their metabolites in different types of materials
Authors:
K. Vrbík 1; A. Vavrouš 1; J. Pavloušková 1; M. Malý 1
; Š. Svačina 2; R. Piecha 2; Z. Lacinová 2; D. Müllerová 3,4; D. Matějková 4; M. Mráz 2
Authors place of work:
Státní zdravotní ústav, Praha
1; 3. interní klinika, 1. lékařská fakulta Univerzity Karlovy a Všeobecná fakultní nemocnice v Praze, Praha
2; Univerzita Karlova v Praze, Lékařská fakulta v Plzni, Ústav hygieny a preventivní medicíny, Plzeň
3; 1. interní klinika Fakultní nemocnice, Plzeň
4
Published in the journal:
Klin. Biochem. Metab., 24, 2016, No. 3, p. 153-159
Summary
Objective:
To apply analytical procedures for the determination of phthalates, bisphenol A and their metabolites in various matrices (urine, blood serum, adipose tissue, infusion sets) to instrumentation available in the lab and perform analysis of samples provided by the clinics. To determine the exposure to phthalates and bisphenol A in different epidemiological groups of patients and to confirm or refute the likely exposure to these substances during parenteral nutrition. To find out whether phthalates accumulate in human adipose tissue.
Design:
A cross-sectional study
Material and Methods:
The study analyzed biological material (urine, blood serum, subcutaneous adipose tissue) and infusion sets for parenteral treatment by instrumental analytical techniques, allowing to determine the content of phthalates, bisphenol A and their metabolites.
Results:
For 10 out of 16 analyzed metabolites, the resulting amounts found in the urine were in the range above the limit of quantitation in more than 50 % of the samples, e.g. monoethyl phthalate – 98.1 % and mono n butyl phthalate 78.1 % of samples. The highest amount characterized by 95th percentile was recorded for monoethyl phthalate – 192.8, mono-n-butyl phthalate – 116.7, and for mono-(2-ethyl-5-carboxypentyl) phthalate – 96.9 µg/L. In serum, the proportion of values lower than limit of quantitation was high except for mono-n-butyl phthalate. All the concentrations of analyzed phthalates and bisphenol A obtained by chromatographic analysis of materials, bags and infusion sets were below limit of quantitation as well as the concentrations in infusion solutions. In all the samples of subcutaneous adipose fat tissue the determined concentrations of selected phthalates were below limit of quantitation.
Conclusion:
The concentrations of most metabolites in urine are measurable which makes possible their use in the analyses of epidemiological link to other investigated factors as obesity, or type 2 diabetes mellitus. Increased exposure to phthalates and bisphenol A for patients on total parenteral therapy has not been proven since those substances were detected neither in infusion sets materials nor in infusion solutions. Accumulation of selected phthalates in subcutaneous adipose tissue was not detected.
Keywords:
phthalate metabolites, bisphenol A, urine, infusion set, high performance liquid chromatography–tandem mass spectrometry.
Zdroje
1. Nařízení Evropského parlamentu a Rady (ES) č. 1907/2006 ve znění nařízení Komise (ES) č. 552/2009 (REACH)
2. Nařízení Komise (EU) č. 10/2011 ve znění nařízení Komise (EU) č. 321/2011, 1282/2011 a 1183/2012
3. Nařízení Evropského parlamentu a Rady (ES) č. 1223/2009
4. Nařízení Evropského parlamentu a Rady COM (2012) 542, o zdravotnických prostředcích a změně směrnice 2001/83/ES, nařízení (ES) č. 178/2002 a nařízení (ES) č. 1223/2009, 2012
5. European Pharmacopoeia (2014). The European Pharmacopoeia, Ph. Eur. 8th Edition, 2014
6. Babich, M. A. Overwiew of phthalate toxi-city, 2010, ISSN 1098-6596. Dostupné z: doi:10.1017/CBO9781107415324.004
7. SCENIHR Scientific Opinion on the safety of medical devices containing DEHP-plasticized PVC or other plasticizers on neonates and other groups possibly at risk [online], 2015, ISBN 9789279356063. Dostupné z: doi:10.2772/45179
8. Koch, H. M., Preuss, R., Angerer, J., Foster, P., Sharpe, R., Toparri, J. Di(2-ethylhexyl)phthalate (DEHP): Human metabolism and internal exposure - An update and latest results. International Journal of Andrology [online], 2006, roč. 29, č. 1, s. 155–165. ISSN 01056263. Dostupné z: doi:10.1111/j.1365-2605.2005.00607.x
9. Koch H. M., Bolt H. M., Angerer J. Di(2-ethylhexyl)phthalate (DEHP) metabolites in human urine and serum after a single oral dose of deuterium-labelled DEHP. Archives of Toxicology [online], 2004, roč. 78, č. 3, s. 123 130. ISSN 03405761. Dostupné z: doi:10.1007/s00204-003-0522-3
10. Alzaga, R. Determination of phthalic monoesters in aqueous and urine samples by solid-phase microextraction – diazomethane on-fibre derivatization – gas chromatography – mass spectrometry. Biodegradation, 2003, s. 87–96.
11. Preuss, R., Koch, H. M., Angerer, J. Biological monitoring of the five major metabolites of di-(2-ethylhexyl)phthalate (DEHP) in human urine using co-lumn-switching liquid chromatography-tandem mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [online], 2005, roč. 816, č. 1-2, s. 269–280. ISSN 15700232. Dostupné z: doi:10.1016/j.jchromb.2004.11.048
12. Jeong, J. Y., Lee, J. H., Kim, E. Y., Kim, P. G., Kho, Y. L. Determination of Phthalate Metabolites in Human Serum and Urine as Biomarkers for Phthalate Exposure Using Column-Switching LC-MS/MS. Safety and Health at Work [online], 2011, roč. 2, č. 1, s. 57. ISSN 2093-7911. Dostupné z: doi:10.5491/SHAW.2011.2.1.57
13. Kato, K., Shoda, S., Takahashi, M., Doi, N., Yoshimura, Y., Nakazawa, H. Determination of three phthalate metabolites in human urine using on-line solid-phase extraction–liquid chromatography–tandem mass spectrometry. Journal of Chromatography B [online], 2003, roč. 788, č. 2, s. 407–411. ISSN 15700232. Dostupné z: doi:10.1016/S1570-0232(03)00041-2
14. Černá, M., Krsková, A., Malý, M., Tomášková, L., Spěváčková, V. National report on the implementation of the project including data analysis and integrated interpretation Czech Republic – Methods, Version final II, National Institute of Public Health, 2013.
15. Černá, M., Malý, M., Rudnai, P. et al. Case study: Possible differences in phthalates exposure among the Czech, Hungarian, and Slovak populations identified based on the DEMOCOPHES pilot study results. Environmental Research [online], 2015, roč. 141, s. 118–124. ISSN 10960953. Dostupné z: doi:10.1016/j.envres.2014.10.025
16. Vanderberg, L. N., Hauser, R., Marcus, M., Olea, N., Wade, V. Human exposure to BPA. Reproductive Toxicology, 2006, s. 1–86.
17. Směrnice Evropského parlamentu a Rady 2009/48/ES a Směrnice Komise 2014/81/ES
18. Prováděcí Nařízení Komise (EU) č. 321/2011
19. Michałowicz, J. Bisphenol A - Sources, toxicity and biotransformation. Environmental Toxicology and Pharmacology [online], B.m.: Elsevier B.V., 2014, roč. 37, č. 2, s. 738–758. ISSN 18727077. Dostupné z: doi:10.1016/j.etap.2014.02.003
20. Völkel, W., Colnot, T., Csanády, A. G. , Filser, J., Dekant, W. Metabolism and Kinetics of Bisphenol A in Humans at Low Doses Following Oral Administration. Chem. Res. Toxicol., 2002, č. 15 (10), s.1281–1287
21. Blount, B. C., Milgram, K. E., Silva, M. J. et al. Quantitative detection of eight phthalate metabolites in human urine using HPLC-APCI-MS/MS. Analytical chemistry [online], 2000, roč. 72, č. 17, s. 4127–4134. ISSN 0003-2700. Dostupné z: doi:10.1289/ehp.6663
22. Koch, H. M., Gonzales-Reche, L. M., Angerer, J. On-line clean-up by multidimensional liquid chromatography – electrospray ionization tandem mass spectrometry for high throughput quantification of primary and secondary phthalate metabolites in human urine. Journal of Chromatography B., 2003, roč. 784, s. 169–182.
23. Schindler, B. K., Esteban, M., Holger Martin Koch, H. M. et al. The European COPHES/DEMOCOPHES project: Towards transnational comparability and reliabi-lity of human biomonitoring results. International Journal of Hygiene and Environmental Health [online], 2014, roč. 217, č. 6, s. 653–661. ISSN 1618131X. Dostupné z: doi:10.1016/j.ijheh.2013.12.002
24. US Food and Drug Administration Safety assessment of Di-(2-ethylhexyl) phthalate (DEHP) released from PVC medical devices. Center for Devices and Radiological Health, 2001, s. 119.
25. Biedermann-Brem, S., Biedermann, M., Fiselier, K., Grob, K. Compositional GC-FID analysis of the additives to PVC, focusing on the gaskets of lids for glass jars, Food Additives and Contaminants, 2005, roč. 22 (12), 1274-84
26. Hauser, R. Phthalates and Human Health. Occupational and Environmental Medicine [online], 2005, roč. 62, č. 11, s. 806–818. ISSN 1351-0711. Dostupné z: doi:10.1136/oem.2004.017590
27. Krotz, S. P., Carson, S. A., Tomey, C., Buster, E. J. Phthalates and bisphenol do not accumulate in human follicular fluid. Journal of Assisted Reproduction and Genetics [online], 2012, roč. 29, č. 8, s. 773–777. ISSN 10580468. Dostupné z: doi:10.1007/s10815-012-9775-1
28. Mes, J., Coffin, D.E., Campbell, D.S. Di-n-butyl- and di-2-ethylhexyl phthalate in human adipose tissue. Bull Environ Contam Toxicol, 1974, č. 12, s. 721-725
29. Wang, N., Kong, D., Shan, Z. et al. Simultaneous determination of pesticides, polycyclic aromatic hydrocarbons, polychlorinated biphenyls and phthalate esters in human adipose tissue by gas chromatography-tandem mass spectrometry. Journal of Chromatography B, 2012, č. 898, s. 38–52
30. Schneiderka, P., Pacáková, V., Stulík, K., Kloudová, M., Jelínková, K. High-performance liquid chromatographic determination of creatinine in serum, and a correlation of the results with those of the Jaffé and enzymic methods. Journal of Chromatography, 1993, č. 614 (2), s. 221–6.
31. Piecha, R., Svačina, Š., Malý, M. et al. Urine level of phthalate metabolites and bisphenol A in relation to main metabolic syndrome components: dyslipidaemia, hypertension and type 2 diabetes (pilot study) Cent. Eur. J. Public Health, 2016, č. 24. Dostupné z http://dx.doi.org/10.21101/cejph.a4704
32. Frederiksen, H., Jørgensen, N., Andersson, A. M. Correlations between phthalate metabolites in urine, serum, and seminal plasma from young Danish men determined by isotope dilution liquid chromatography tandem mass spectrometry. Journal of analytical toxicology [online]. 2010, roč. 34, č. 7, s. 400–410. ISSN 1945-2403. Dostupné z: doi:10.1038/jes.2010.6
Štítky
Clinical biochemistry Nuclear medicine Nutritive therapistČlánok vyšiel v časopise
Clinical Biochemistry and Metabolism
2016 Číslo 3
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