Proteins of Resistence and Drug Resistence in Ovarian Carcinoma Patients

Česká verzia

Authors: I. Sedláková ¹; J. Laco ²; J. Tošner ¹; K. Caltová ³; M. Červinka ³; A. Řezáč ¹; J. Špaček ¹; P. Škapinec ¹
Authors place of work: Porodnická a gynekologická klinika LF UK a Fakultní nemocnice Hradec Králové, přednosta doc. MUDr. Jindřich Tošner, CSc. ¹; Fingerlandův ústav patologie Fakultní nemocnice Hradec Králové, přednosta prof. MUDr. Aleš Ryška, PhD. ²; Ústav biologie a lékařské genetiky Lékařské fakulty Hradec Králové UK v Praze, přednosta prof. MUDr. RNDr. Miroslav Červinka, CSc. ³
Published in the journal: Klin Onkol 2012; 25(6): 457-463
Category: Original Articles

Práce byla podpořena grantem IGA MZ ČR NS 9737-3.

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Summary

Background:
To evaluate the correlation of resistance proteins LRP (Lung Resistance Protein), Pgp (P-glycoprotein), MRP (Multidrug Resistance-Associated Protein), MRP3 a MRP5 with stage, grade and histological type. To asses correlation of these resistance proteins with drug resistance/drug sensitivity in vitro by means of the MTT assay in ovarian cancer patients. To find the clinical outcome of these data.

Patients and Methods:
64 women with epithelial ovarian cancer who underwent primary surgery in 2006–2010 had specimens stained with imunohistochemistry for LRP, MRP, MRP3, MRP5 and Pgp and MTT assay (MTT-(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide).

Results:
Patients with late ovarian cancer had a higher Pgp, MRP, MRP3 and MRP5 level compared to ovarian cancer patients with early stage ovarian cancer. No correlation of resistance proteins with grading was found. Patients with high Pgp and MRP expression had significantly shorter progression-free survival. Patients with drug resistance in vitro by means of the MTT assay had higher Pgp and MRP expression.

Conclusion:
P-glycoprotein and MRP may be useful predictor for outcome of primary chemotherapy in patients with ovarian cancer.

Key words:
ovarian cancer – p170-glycoprotein – Lrp-Lung resistance protein – MRP1 protein – Drug Resistance – MTT test

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