Expression Profiling during Arabidopsis/Downy Mildew Interaction Reveals a Highly-Expressed Effector That Attenuates Responses to Salicylic Acid

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A comprehensive understanding of host-pathogen interactions requires knowledge of the dynamics of gene expression changes in both the host and the pathogen during a time course of infection. However, expression profiling has often focused on either the host or the pathogen due to limitations of methods that involve microarrays. We report here gene expression changes in both Arabidopsis and its parasite Hyaloperonospora arabidopsidis (Hpa) simultaneously during infection using a high-throughput RNA sequencing method. By resequencing Hpa isolate Waco9, we found it evades Arabidopsis resistance gene RPP1 through deletion of cognate recognized effector ATR1. We also found that Hpa suppresses responsiveness to salicylic acid (SA) in haustoriated cells into which host-translocated effectors are delivered. An Hpa effector HaRxL62, previously shown to enhance host susceptibility, was highly expressed in this assay, and we found it suppresses responsiveness to SA. Expression profiling of both pathogen effector genes and host genes involved in immunity allows us to suggest distinct mechanisms of effector-mediated susceptibility and reveals interesting Hpa effectors for detailed mechanistic investigation in future experiments.

Vyšlo v časopise: Expression Profiling during Arabidopsis/Downy Mildew Interaction Reveals a Highly-Expressed Effector That Attenuates Responses to Salicylic Acid. PLoS Pathog 10(10): e32767. doi:10.1371/journal.ppat.1004443
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004443

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