The Operon Essential for Biofilm and Rugose Colony Development in

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Biofilms are specialized and highly differentiated three-dimensional communities of bacteria encased in an extracellular polymeric matrix (EPM), and the bacteria’s mechanisms to form biofilm are closely linked to their virulence. The EPM often consists of polysaccharides, proteins, nucleic acids, and lipids. Compared to extracellular polysaccharides, little is known about the protein components in the biofilm matrix of Vibrio vulnificus, a foodborne pathogen. In this study, we identified and characterized cabABC genes which were preferentially expressed in biofilms. CabA is a calcium-binding protein and is secreted through functional CabB and CabC. Our results indicated that CabA contributes to the development of biofilm and rugose colony morphology under elevated c-di-GMP conditions. CabA is an extracellular matrix protein crucial for the structural integrity of robust biofilm in flow cells and on oyster shells. Calcium binding induces conformational changes and multimerization of CabA that may render the protein functional to build a well-structured matrix. CabA can assemble a functional matrix extracellularly only when exopolysaccharides (EPS) coexist, indicating that both CabA and EPS are required for the scaffold of V. vulnificus biofilm matrix. This is the first report on a non-polysaccharide matrix component that is essential for the development of the V. vulnificus biofilm structure.

Vyšlo v časopise: The Operon Essential for Biofilm and Rugose Colony Development in. PLoS Pathog 11(9): e32767. doi:10.1371/journal.ppat.1005192
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005192

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