Is the Assessment of Serum Creatinine Reliable?
Authors:
K. Kotaška
; B. Jedličková; R. Průša
Authors place of work:
Ústav klinické biochemie a patobiochemie 2. LF UK a FNM, Praha
Published in the journal:
Čas. Lék. čes. 2008; 147: 392-395
Category:
Case Report
Summary
Chemical and enzymatic methods are used to measure creatinine in serum and urine. Chemical methods are mostly based on the reaction of creatinine with alkaline picrate (Jaffe reaction). The Jaffe reaction is not specific for creatinine, the same reaction resulting in Jaffe-like chromogens show many interfering substances (ascorbic acid, bilirubin, proteins, ketones, cephalosporins and other drugs). Chemical and enzymatic methods show similar accuracy and day-to-day precision. Chemical methods are cheaper than enzymatic methods. Enzymatic methods require low sample volume and are not affected by the interfering substances as the chemical methods. Presented case report shows an unusual occurrence of drug interference in the enzymatic creatine deaminase procedure. Biological factors (circadian rhythm, pregnancy, hemodialysis, transplantation, stress, exercise), analytical and preanalytical factors (pH, glucose, pyruvate, bilirubin, fatty acids, sample storage and sample collection – gel tubes) and biological variability of creatinine play significant role in the creatinine examination.
Key words:
creatinine, biological variability, interference.
Zdroje
1. Burtis, C. A., Ashwood E. R.: Tietz Textbook of Clinical Chemistry, 3rd Edition, WB Saunders, Philadelphia, 1997, 1900 s.
2. Ricós C., Alvarez V., Cava F. et al.: Current databases on biologic variation pros, cons, and progress. Scand. J. Clin. Lab. Invest., 1999, 59, s. 491–500.
3. Fraser C. G.: Biological Variation from principles to practice. AACC Press© 2001.
4. Chronolab, The Quality of Diagnostic Samples, Chronolab AG, Switzerland 2000–2007 (CD-ROM).
5. Young, D. S.: Effects of Preanalytical Variables on Clinical Laboratory Tests, 2nd Edition, AACC Press©, 1997.
Štítky
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental HygienistČlánok vyšiel v časopise
Journal of Czech Physicians
- Metamizole at a Glance and in Practice – Effective Non-Opioid Analgesic for All Ages
- Advances in the Treatment of Myasthenia Gravis on the Horizon
- Metamizole vs. Tramadol in Postoperative Analgesia
- Spasmolytic Effect of Metamizole
- What Effect Can Be Expected from Limosilactobacillus reuteri in Mucositis and Peri-Implantitis?
Najčítanejšie v tomto čísle
- Is the Assessment of Serum Creatinine Reliable?
- Allergen Challenge Used to Diagnose Food Allergy – Oral Allergy Syndrome
- Relation Between Insulin Resistance and Amount of Visceral Adipose Tissue
- Conduct Disorders in Seven-year-old Children – Results of ELSPAC Study. 3. Postnatal Development