Genetic Analysis of a Novel Tubulin Mutation That Redirects Synaptic Vesicle Targeting and Causes Neurite Degeneration in

English version České info

Axons and dendrites are two classes of neuronal process that differ in their functions and molecular compositions. Proteins important for synaptic functions are mostly synthesized in the cell body and sorted differentially into the axon or dendrites. Microtubules in the axon and dendrite maintain their structural integrity and regulate polarized protein transport into these compartments. We identified a novel α-tubulin mutation in C. elegans that caused mistargeting of synaptic vesicles and induced progressive neurite swelling, which resulted in late-onset neurodegeneration. We showed that this tubulin mutation weakened microtubule network and abnormally increased microtubule affinity for dynein, a motor protein responsible for cargo sorting to the dendrite. This enhanced microtubule-dynein affinity is due to augmented negative charge at the carboxyl terminus of α-tubulin. Neurite swelling and neurodegeneration could be ameliorated by reduced physical activity, suggesting that recurrent mechanical strain from muscle contraction jeopardized neurite integrity in the long run. Mutations in α- and β-tubulins are found in human neurological diseases; our findings therefore contribute to understanding the pathogenic mechanism of human neurological diseases associated with tubulin mutations.

Vyšlo v časopise: Genetic Analysis of a Novel Tubulin Mutation That Redirects Synaptic Vesicle Targeting and Causes Neurite Degeneration in. PLoS Genet 10(11): e32767. doi:10.1371/journal.pgen.1004715
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004715

Souhrn

Zdroje

1. HirokawaN, NiwaS, TanakaY (2010) Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease. Neuron 68: 610–638.

2. CondeC, CaceresA (2009) Microtubule assembly, organization and dynamics in axons and dendrites. Nat Rev Neurosci 10: 319–332.

3. KapiteinLC, SchlagerMA, KuijpersM, WulfPS, van SpronsenM, et al. (2010) Mixed microtubules steer dynein-driven cargo transport into dendrites. Curr Biol 20: 290–299.

4. RollsMM (2011) Neuronal polarity in Drosophila: sorting out axons and dendrites. Dev Neurobiol 71: 419–429.

5. ManiarTA, KaplanM, WangGJ, ShenK, WeiL, et al. (2011) UNC-33 (CRMP) and ankyrin organize microtubules and localize kinesin to polarize axon-dendrite sorting. Nat Neurosci 15: 48–56.

6. YanJ, ChaoDL, TobaS, KoyasakoK, YasunagaT, et al. (2013) Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans. Elife 2: e00133.

7. OuCY, PoonVY, MaederCI, WatanabeS, LehrmanEK, et al. (2010) Two cyclin-dependent kinase pathways are essential for polarized trafficking of presynaptic components. Cell 141: 846–858.

8. GoodwinPR, SasakiJM, JuoP (2012) Cyclin-dependent kinase 5 regulates the polarized trafficking of neuropeptide-containing dense-core vesicles in Caenorhabditis elegans motor neurons. J Neurosci 32: 8158–8172.

9. KoushikaSP, SchaeferAM, VincentR, WillisJH, BowermanB, et al. (2004) Mutations in Caenorhabditis elegans cytoplasmic dynein components reveal specificity of neuronal retrograde cargo. J Neurosci 24: 3907–3916.

10. MizunoN, TobaS, EdamatsuM, Watai-NishiiJ, HirokawaN, et al. (2004) Dynein and kinesin share an overlapping microtubule-binding site. EMBO J 23: 2459–2467.

11. KikkawaM, HirokawaN (2006) High-resolution cryo-EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations. EMBO J 25: 4187–4194.

12. RedwineWB, Hernandez-LopezR, ZouS, HuangJ, Reck-PetersonSL, et al. (2012) Structural basis for microtubule binding and release by dynein. Science 337: 1532–1536.

13. UchimuraS, OguchiY, HachikuboY, IshiwataS, MutoE (2010) Key residues on microtubule responsible for activation of kinesin ATPase. EMBO J 29: 1167–1175.

14. TischfieldMA, BarisHN, WuC, RudolphG, Van MaldergemL, et al. (2010) Human TUBB3 mutations perturb microtubule dynamics, kinesin interactions, and axon guidance. Cell 140: 74–87.

15. NiwaS, TakahashiH, HirokawaN (2013) beta-Tubulin mutations that cause severe neuropathies disrupt axonal transport. EMBO J 32: 1352–1364.

16. Chalfie M.; SulstonJ (1981) Development Genetics of the Mechanosensory Neurons of C. elegans. Dev Biol 82: 358–370.

17. FukushigeT, SiddiquiZK, ChouM, CulottiJG, GogoneaCB, et al. (1999) MEC-12, an alpha-tubulin required for touch sensitivity in C. elegans. J Cell Sci 112: 395–403.

18. GoodmanMB (2006) Mechanosensation. WormBook 6: 1–14.

19. Bounoutas A, Zheng Q, Nonet ML, Chalfie M (2009) mec-15 encodes an F-box protein required for touch receptor neuron mechanosensation, synapse formation and development. Genetics 183: : 607–617, 601SI-604SI.

20. ZhengQ, SchaeferAM, NonetML (2011) Regulation of C. elegans presynaptic differentiation and neurite branching via a novel signaling pathway initiated by SAM-10. Development 138: 87–96.

21. TopalidouI, KellerC, KalebicN, NguyenKC, SomhegyiH, et al. (2012) Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization. Curr Biol 22: 1057–1065.

22. KriegM, DunnAR, GoodmanMB (2014) Mechanical control of the sense of touch by beta-spectrin. Nat Cell Biol 16: 224–233.

23. PanCL, PengCY, ChenCH, McIntireS (2011) Genetic analysis of age-dependent defects of the Caenorhabditis elegans touch receptor neurons. Proc Natl Acad Sci U S A 108: 9274–9279.

24. SiddiquiSS, AamodtE, RastinejadF, CulottiJ (1989) Anti-tubulin monoclonal antibodies that bind to specific neurons in Caenorhabditis elegans. J Neurosci 9: 2963–2972.

25. SavageC, XueY, MitaniS, HallD, ZakharyR, et al. (1994) Mutations in the Caenorhabditis elegans beta-tubulin gene mec-7: effects on microtubule assembly and stability and on tubulin autoregulation. J Cell Sci 107: 2165–2175.

26. Ghosh-RoyA, GoncharovA, JinY, ChisholmAD (2012) Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration. Dev Cell 23: 716–728.

27. NeumannBH, MA (2014) Loss of MEC-17 Leads to Microtubule Instability and Axonal Degeneration. Cell Reports 6: 93–103.

28. KimuraY, KurabeN, IkegamiK, TsutsumiK, KonishiY, et al. (2010) Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs). J Biol Chem 285: 22936–22941.

29. O'HaganR, PiaseckiBP, SilvaM, PhirkeP, NguyenKC, et al. (2011) The tubulin deglutamylase CCPP-1 regulates the function and stability of sensory cilia in C. elegans. Curr Biol 21: 1685–1694.

30. GonczyP, PichlerS, KirkhamM, HymanAA (1999) Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo. J Cell Biol 147: 135–150.

31. KonishiY, SetouM (2009) Tubulin tyrosination navigates the kinesin-1 motor domain to axons. Nat Neurosci 12: 559–567.

32. IkegamiK, HeierRL, TaruishiM, TakagiH, MukaiM, et al. (2007) Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci U S A 104: 3213–3218.

33. GoshimaY, NakamuraF, StrittmatterP, StrittmatterSM (1995) Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33. Nature 376: 509–514.

34. OtsukaAJ, FrancoR, YangB, ShimKH, TangLZ, et al. (1995) An ankyrin-related gene (unc-44) is necessary for proper axonal guidance in Caenorhabditis elegans. J Cell Biol 129: 1081–1092.

35. TischfieldMA, CederquistGY, GuptaMLJr, EngleEC (2011) Phenotypic spectrum of the tubulin-related disorders and functional implications of disease-causing mutations. Curr Opin Genet Dev 21: 286–294.

36. SimonsC, WolfNI, McNeilN, CaldovicL, DevaneyJM, et al. (2013) A de novo mutation in the beta-tubulin gene TUBB4A results in the leukoencephalopathy hypomyelination with atrophy of the basal ganglia and cerebellum. Am J Hum Genet 92: 767–773.

37. LohmannK, WilcoxRA, WinklerS, RamirezA, RakovicA, et al. (2013) Whispering dysphonia (DYT4 dystonia) is caused by a mutation in the TUBB4 gene. Ann Neurol 73: 537–545.

38. KeaysDA, TianG, PoirierK, HuangGJ, SieboldC, et al. (2007) Mutations in alpha-tubulin cause abnormal neuronal migration in mice and lissencephaly in humans. Cell 128: 45–57.

39. HammarlundM, JorgensenEM, BastianiMJ (2007) Axons break in animals lacking beta-spectrin. J Cell Biol 176: 269–275.

40. BrennerS (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71–94.

41. MelloCC, KramerJM, StinchcombD, AmbrosV (1991) Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J 10: 3959–3970.

42. HilliardMA, BargmannCI (2006) Wnt signals and frizzled activity orient anterior-posterior axon outgrowth in C. elegans. Dev Cell 10: 379–390.

43. PrasadBC, ClarkSG (2006) Wnt signaling establishes anteroposterior neuronal polarity and requires retromer in C. elegans. Development 133: 1757–1766.

44. PanCL, BaumPD, GuM, JorgensenEM, ClarkSG, et al. (2008) C. elegans AP-2 and retromer control Wnt signaling by regulating mig-14/Wntless. Dev Cell 14: 132–139.

45. DavisMW, HammarlundM, HarrachT, HullettP, OlsenS, et al. (2005) Rapid single nucleotide polymorphism mapping in C. elegans. BMC Genomics 6: 118.

46. KamathRS, Martinez-CamposM, ZipperlenP, FraserAG, AhringerJ (2001) Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol 2 RESEARCH0002.

47. CalixtoA, ChelurD, TopalidouI, ChenX, ChalfieM (2010) Enhanced neuronal RNAi in C. elegans using SID-1. Nat Methods 7: 554–559.

48. McDonaldKL, WebbRI (2011) Freeze substitution in 3 hours or less. J Microsc 243: 227–233.

49. ReynoldsES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17: 208–212.

50. FinneyM, RuvkunG (1990) The unc-86 gene product couples cell lineage and cell identity in C. elegans. Cell 63: 895–905.

51. LyeRJ, PorterME, ScholeyJM, McIntoshJR (1987) Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans. Cell 51: 309–318.

52. KumarJ, ChoudharyBC, MetpallyR, ZhengQ, NonetML, et al. (2010) The Caenorhabditis elegans Kinesin-3 motor UNC-104/KIF1A is degraded upon loss of specific binding to cargo. PLoS Genet 6: e1001200.