Accounting for Experimental Noise Reveals That mRNA Levels, Amplified by Post-Transcriptional Processes, Largely Determine Steady-State Protein Levels in Yeast

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Cells respond to their environment by making proteins using transcription and translation of mRNA. Modest observed correlations between global steady-state mRNA and protein measurements have been interpreted as evidence that mRNA levels determine roughly 40% of the variation in protein levels, indicating dominant post-transcriptional effects. However, the techniques underlying these conclusions, such as correlation and regression, yield biased results when data are noisy and contain missing values. Here we show that when methods that account for noise are used to analyze much of the same data, mRNA levels explain more than 85% of the variation in steady-state protein levels. Protein levels are not proportional to mRNA levels as commonly assumed, but rise much more rapidly. Regulation of translation achieves amplification of, rather than competition with, transcriptional signals. Our results suggest that for this set of conditions, mRNA sets protein-level regulation, and introduce multiple noise-aware approaches essential for proper analysis of many biological phenomena.

Vyšlo v časopise: Accounting for Experimental Noise Reveals That mRNA Levels, Amplified by Post-Transcriptional Processes, Largely Determine Steady-State Protein Levels in Yeast. PLoS Genet 11(5): e32767. doi:10.1371/journal.pgen.1005206
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005206

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