Dissecting the Function and Assembly of Acentriolar Microtubule Organizing Centers in Cells In Vivo

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During cell division, chromosomes are divided into two daughter cells by the mitotic spindle, a complex structure made from microtubules. The correct formation of the mitotic spindle is essential, as missegregation of chromosomes can lead to cell death or cancer. Therefore several mechanisms cooperate in nucleating the microtubules needed for the mitotic spindle and focusing them into a bipolar structure. One of these mechanisms, which has only recently been identified, is microtubule nucleation by acentriolar microtubule organizing centers (aMTOCs). These structures have been observed in several cell types, notably also in cancer cells, but is not known how they are formed and which function they might have in mitotic spindle assembly. We identified the pathway of aMTOC formation in Drosophila, which enabled us to perturb their formation in order to study their role during spindle formation. We show that aMTOCs are a source of microtubule nucleation, but their contribution to spindle formation is normally masked by other, more dominant, pathways of microtubule nucleation. Furthermore, we have identified a role for aMTOCs in focusing of mitotic spindle poles by the molecular motor dynein in cells in which centrioles are missing.

Vyšlo v časopise: Dissecting the Function and Assembly of Acentriolar Microtubule Organizing Centers in Cells In Vivo. PLoS Genet 11(5): e32767. doi:10.1371/journal.pgen.1005261
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005261

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