A Year of Infection in the Intensive Care Unit: Prospective Whole Genome Sequencing of Bacterial Clinical Isolates Reveals Cryptic Transmissions and Novel Microbiota

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Bacterial whole genome sequencing is becoming increasingly common to microbiological research, but despite its great potential, has not yet been meaningfully integrated into clinical care. Here, we generated whole genome sequencing data from nearly all of the bacterial isolates prospectively collected from a hospital’s intensive care units over an entire year. Our analysis identifies novel microbiota in hospitalized patients, a high incidence of patient infection with multiple unrelated lineages of a bacterial species, and the possibility of cryptic transmission of bacteria among patients. Our study is unprecedented in providing a broad and unbiased view of bacterial infections that affect the hospital’s sickest patients, and demonstrates the extent of information that can be learned from comprehensive genomic surveillance of clinical bacterial isolates over an extended period of time.

Vyšlo v časopise: A Year of Infection in the Intensive Care Unit: Prospective Whole Genome Sequencing of Bacterial Clinical Isolates Reveals Cryptic Transmissions and Novel Microbiota. PLoS Genet 11(7): e32767. doi:10.1371/journal.pgen.1005413
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005413

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