#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Odborné časopisy / PLOS Pathogens / 2014 - 8

Production of an Attenuated Phenol-Soluble Modulin Variant Unique to the MRSA Clonal Complex 30 Increases Severity of Bloodstream Infection

English version České info

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of morbidity and mortality and a great concern for public health. The CC30 MRSA lineage is especially notorious for causing bloodstream infections with complications such as seeding into organs. In our study, we show that this lineage produces an attenuated form of a key S. aureus toxin with decreased pro-inflammatory features. Our results suggest that attenuation of this toxin allows the bacteria to evade recognition and subsequent elimination by host defenses, thereby increasing pathogen success during blood infection.

Vyšlo v časopise: Production of an Attenuated Phenol-Soluble Modulin Variant Unique to the MRSA Clonal Complex 30 Increases Severity of Bloodstream Infection. PLoS Pathog 10(8): e32767. doi:10.1371/journal.ppat.1004298
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004298

Souhrn

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of morbidity and mortality and a great concern for public health. The CC30 MRSA lineage is especially notorious for causing bloodstream infections with complications such as seeding into organs. In our study, we show that this lineage produces an attenuated form of a key S. aureus toxin with decreased pro-inflammatory features. Our results suggest that attenuation of this toxin allows the bacteria to evade recognition and subsequent elimination by host defenses, thereby increasing pathogen success during blood infection.

Zdroje

1. LowyFD (1998) Staphylococcus aureus infections. N Engl J Med 339: 520–532.

2. FosterTJ (2005) Immune evasion by staphylococci. Nat Rev Microbiol 3: 948–958.

3. OttoM (2012) MRSA virulence and spread. Cell Microbiol 14: 1513–1521.

4. OttoM (2013) Community-associated MRSA: what makes them special? Int J Med Microbiol 303: 324–330.

5. CoxRA, ConquestC, MallaghanC, MarplesRR (1995) A major outbreak of methicillin-resistant Staphylococcus aureus caused by a new phage-type (EMRSA-16). J Hosp Infect 29: 87–106.

6. JohnsonAP, AuckenHM, CavendishS, GannerM, WaleMC, et al. (2001) Dominance of EMRSA-15 and -16 among MRSA causing nosocomial bacteraemia in the UK: analysis of isolates from the European Antimicrobial Resistance Surveillance System (EARSS). J Antimicrob Chemother 48: 143–144.

7. McAdamPR, TempletonKE, EdwardsGF, HoldenMT, FeilEJ, et al. (2012) Molecular tracing of the emergence, adaptation, and transmission of hospital-associated methicillin-resistant Staphylococcus aureus. Proc Natl Acad Sci U S A 109: 9107–9112.

8. FowlerVGJr, NelsonCL, McIntyreLM, KreiswirthBN, MonkA, et al. (2007) Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection. J Infect Dis 196: 738–747.

9. DeLeoFR, KennedyAD, ChenL, Bubeck WardenburgJ, KobayashiSD, et al. (2011) Molecular differentiation of historic phage-type 80/81 and contemporary epidemic Staphylococcus aureus. Proc Natl Acad Sci U S A 108: 18091–18096.

10. PeschelA, OttoM (2013) Phenol-soluble modulins and staphylococcal infection. Nat Rev Microbiol 11: 667–673.

11. WangR, BraughtonKR, KretschmerD, BachTH, QueckSY, et al. (2007) Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat Med 13: 1510–1514.

12. CheungGY, DuongAC, OttoM (2012) Direct and synergistic hemolysis caused by Staphylococcus phenol-soluble modulins: implications for diagnosis and pathogenesis. Microbes Infect 14: 380–386.

13. RasigadeJP, Trouillet-AssantS, FerryT, DiepBA, SapinA, et al. (2013) PSMs of hypervirulent Staphylococcus aureus act as intracellular toxins that kill infected osteoblasts. PLoS One 8: e63176.

14. KretschmerD, GleskeAK, RautenbergM, WangR, KoberleM, et al. (2010) Human formyl peptide receptor 2 senses highly pathogenic Staphylococcus aureus. Cell Host Microbe 7: 463–473.

15. CheungGY, JooHS, ChatterjeeSS, OttoM (2013) Phenol-soluble modulins - critical determinants of staphylococcal virulence. FEMS Microbiol Rev doi: 10.1111/1574-6976.12057. [Epub ahead of print]

16. GravesSF, KobayashiSD, BraughtonKR, WhitneyAR, SturdevantDE, et al. (2012) Sublytic concentrations of Staphylococcus aureus Panton-Valentine leukocidin alter human PMN gene expression and enhance bactericidal capacity. J Leukoc Biol 92: 361–374.

17. JooHS, OttoM (2014) The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis. Methods Mol Biol 1106: 93–100.

18. HoldenMT, FeilEJ, LindsayJA, PeacockSJ, DayNP, et al. (2004) Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance. Proc Natl Acad Sci U S A 101: 9786–9791.

19. QueckSY, Jameson-LeeM, VillaruzAE, BachTH, KhanBA, et al. (2008) RNAIII-independent target gene control by the agr quorum-sensing system: insight into the evolution of virulence regulation in Staphylococcus aureus. Mol Cell 32: 150–158.

20. TraberKE, LeeE, BensonS, CorriganR, CanteraM, et al. (2008) agr function in clinical Staphylococcus aureus isolates. Microbiology 154: 2265–2274.

21. NovickRP, RossHF, ProjanSJ, KornblumJ, KreiswirthB, et al. (1993) Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J 12: 3967–3975.

22. JooHS, CheungGY, OttoM (2011) Antimicrobial activity of community-associated methicillin-resistant Staphylococcus aureus is caused by phenol-soluble modulin derivatives. J Biol Chem 286: 8933–8940.

23. PratC, BestebroerJ, de HaasCJ, van StrijpJA, van KesselKP (2006) A new staphylococcal anti-inflammatory protein that antagonizes the formyl peptide receptor-like 1. J Immunol 177: 8017–8026.

24. de HaasCJ, VeldkampKE, PeschelA, WeerkampF, Van WamelWJ, et al. (2004) Chemotaxis inhibitory protein of Staphylococcus aureus, a bacterial antiinflammatory agent. J Exp Med 199: 687–695.

25. DuongAC, CheungGY, OttoM (2012) Interaction of phenol-soluble modulins with phosphatidylcholine vesicles. Pathogens 1: 3–11.

26. KobayashiSD, MalachowaN, WhitneyAR, BraughtonKR, GardnerDJ, et al. (2011) Comparative analysis of USA300 virulence determinants in a rabbit model of skin and soft tissue infection. J Infect Dis 204: 937–941.

27. SurewaardBG, de HaasCJ, VervoortF, RigbyKM, DeLeoFR, et al. (2013) Staphylococcal alpha-phenol soluble modulins contribute to neutrophil lysis after phagocytosis. Cell Microbiol 15: 1427–1437.

28. SpaanAN, SurewaardBG, NijlandR, van StrijpJA (2013) Neutrophils versus Staphylococcus aureus: a biological tug of war. Annu Rev Microbiol 67: 629–650.

29. HolzingerD, GieldonL, MysoreV, NippeN, TaxmanDJ, et al. (2012) Staphylococcus aureus Panton-Valentine leukocidin induces an inflammatory response in human phagocytes via the NLRP3 inflammasome. J Leukoc Biol 92: 1069–1081.

30. YoongP, PierGB (2012) Immune-activating properties of Panton-Valentine leukocidin improve the outcome in a model of methicillin-resistant Staphylococcus aureus pneumonia. Infect Immun 80: 2894–2904.

31. HermosCR, YoongP, PierGB (2010) High levels of antibody to Panton-Valentine leukocidin are not associated with resistance to Staphylococcus aureus-associated skin and soft-tissue infection. Clin Infect Dis 51: 1138–1146.

32. YoongP, PierGB (2010) Antibody-mediated enhancement of community-acquired methicillin-resistant Staphylococcus aureus infection. Proc Natl Acad Sci U S A 107: 2241–2246.

33. FowlerVGJr, SakoulasG, McIntyreLM, MekaVG, ArbeitRD, et al. (2004) Persistent bacteremia due to methicillin-resistant Staphylococcus aureus infection is associated with agr dysfunction and low-level in vitro resistance to thrombin-induced platelet microbicidal protein. J Infect Dis 190: 1140–1149.

34. PaulanderW, Nissen VarmingA, BaekKT, HaaberJ, FreesD, et al. (2013) Antibiotic-mediated selection of quorum-sensing-negative Staphylococcus aureus. mBio 3: e00459-00412.

35. CheungGY, KretschmerD, QueckSY, JooHS, WangR, et al. (2014) Insight into structure-function relationship in phenol-soluble modulins using an alanine screen of the phenol-soluble modulin (PSM) alpha3 peptide. FASEB J 28: 153–161.

36. ChatterjeeSS, JooHS, DuongAC, DieringerTD, TanVY, et al. (2013) Essential Staphylococcus aureus toxin export system. Nat Med 19: 364–367.

37. BaeT, SchneewindO (2006) Allelic replacement in Staphylococcus aureus with inducible counter-selection. Plasmid 55: 58–63.

38. PeschelA, OttenwalderB, GotzF (1996) Inducible production and cellular location of the epidermin biosynthetic enzyme EpiB using an improved staphylococcal expression system. FEMS Microbiol Lett 137: 279–284.

39. VoyichJM, OttoM, MathemaB, BraughtonKR, WhitneyAR, et al. (2006) Is Panton-Valentine leukocidin the major virulence determinant in community-associated methicillin-resistant Staphylococcus aureus disease? J Infect Dis 194: 1761–1770.

Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium
Článek Regulatory RNAs Involved in Bacterial Antibiotic Resistance
Článek From Dandruff to Deep-Sea Vents: -like Fungi Are Ecologically Hyper-diverse
Článek Pathogenicity and Epithelial Immunity
Článek Mother–Infant HIV Transmission: Do Maternal HIV-Specific Antibodies Protect the Infant?
Článek Hell's BELs: acterial 3 igases That Exploit the Eukaryotic Ubiquitin Machinery
Článek Disruption of Fas-Fas Ligand Signaling, Apoptosis, and Innate Immunity by Bacterial Pathogens
Článek Primary Seronegative but Molecularly Evident Hepadnaviral Infection Engages Liver and Induces Hepatocarcinoma in the Woodchuck Model of Hepatitis B
Článek TLR2 Signaling Decreases Transmission of by Limiting Bacterial Shedding in an Infant Mouse Influenza A Co-infection Model
Článek Inhibition of the TRAIL Death Receptor by CMV Reveals Its Importance in NK Cell-Mediated Antiviral Defense
Článek Early Mucosal Sensing of SIV Infection by Paneth Cells Induces IL-1β Production and Initiates Gut Epithelial Disruption
Článek Limited HIV Infection of Central Memory and Stem Cell Memory CD4+ T Cells Is Associated with Lack of Progression in Viremic Individuals
Článek Virus-Specific Regulatory T Cells Ameliorate Encephalitis by Repressing Effector T Cell Functions from Priming to Effector Stages
Článek A Tick Gut Protein with Fibronectin III Domains Aids Congregation to the Gut during Transmission
Článek The HIV-1 Envelope Transmembrane Domain Binds TLR2 through a Distinct Dimerization Motif and Inhibits TLR2-Mediated Responses
Článek Infection with MERS-CoV Causes Lethal Pneumonia in the Common Marmoset
Článek VGIII Isolates Causing Infections in HIV/AIDS Patients in Southern California: Identification of the Local Environmental Source as Arboreal
Článek Diverse Host-Seeking Behaviors of Skin-Penetrating Nematodes
Článek Capsid Protein VP4 of Human Rhinovirus Induces Membrane Permeability by the Formation of a Size-Selective Multimeric Pore
Článek The Murine Gammaherpesvirus Immediate-Early Rta Synergizes with IRF4, Targeting Expression of the Viral M1 Superantigen to Plasma Cells
Článek Characterization of an Insecticidal Toxin and Pathogenicity of against Insects
Článek The Vi Capsular Polysaccharide Enables Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis
Článek Histone Deacetylase Inhibitors Impair the Elimination of HIV-Infected Cells by Cytotoxic T-Lymphocytes
Článek A Locus Encompassing the Epstein-Barr Virus Kinase Regulates Expression of Genes Encoding Viral Structural Proteins
Článek Distinct APC Subtypes Drive Spatially Segregated CD4 and CD8 T-Cell Effector Activity during Skin Infection with HSV-1
Článek Structure of CfaA Suggests a New Family of Chaperones Essential for Assembly of Class 5 Fimbriae
Článek Adoptive Transfer of EBV Specific CD8 T Cell Clones Can Transiently Control EBV Infection in Humanized Mice
Článek Schistosome Feeding and Regurgitation
Článek EVM005: An Ectromelia-Encoded Protein with Dual Roles in NF-κB Inhibition and Virulence
Článek Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery
Článek Why HIV Virions Have Low Numbers of Envelope Spikes: Implications for Vaccine Development
Článek Identification of Anti-virulence Compounds That Disrupt Quorum-Sensing Regulated Acute and Persistent Pathogenicity
Článek HIV-1 Receptor Binding Site-Directed Antibodies Using a VH1-2 Gene Segment Orthologue Are Activated by Env Trimer Immunization
Článek Cooperation between Epstein-Barr Virus Immune Evasion Proteins Spreads Protection from CD8 T Cell Recognition across All Three Phases of the Lytic Cycle
Článek Parasite Extracellular Vesicles: Mediators of Intercellular Communication
Článek RC1339/APRc from Is a Novel Aspartic Protease with Properties of Retropepsin-Like Enzymes
Článek Cyclic di-GMP-dependent Signaling Pathways in the Pathogenic Firmicute
Článek Non-random Escape Pathways from a Broadly Neutralizing Human Monoclonal Antibody Map to a Highly Conserved Region on the Hepatitis C Virus E2 Glycoprotein Encompassing Amino Acids 412–423
Článek Neutrophil Elastase Causes Tissue Damage That Decreases Host Tolerance to Lung Infection with Species
Článek Ly6C Monocyte Recruitment Is Responsible for Th2 Associated Host-Protective Macrophage Accumulation in Liver Inflammation due to Schistosomiasis
Článek SGNH Hydrolase-Like Proteins AlgJ and AlgX Have Similar Topology but Separate and Distinct Roles in Alginate Acetylation
Článek Why Sexually Transmitted Infections Tend to Cause Infertility: An Evolutionary Hypothesis
Článek Late Engagement of CD86 after Influenza Virus Clearance Promotes Recovery in a FoxP3 Regulatory T Cell Dependent Manner
Článek Determinants of Influenza Transmission in South East Asia: Insights from a Household Cohort Study in Vietnam
Článek A Novel Signal Transduction Pathway that Modulates Quorum Sensing and Bacterial Virulence in
Článek Host Responses to Group A Streptococcus: Cell Death and Inflammation
Článek A Cysteine Protease Inhibitor of Is Essential for Exo-erythrocytic Development
Článek EBNA3C Augments Pim-1 Mediated Phosphorylation and Degradation of p21 to Promote B-Cell Proliferation
Článek On the Front Line: Quantitative Virus Dynamics in Honeybee ( L.) Colonies along a New Expansion Front of the Parasite
Článek Assembly and Architecture of the EBV B Cell Entry Triggering Complex
Článek NLR-Associating Transcription Factor bHLH84 and Its Paralogs Function Redundantly in Plant Immunity
Článek The PDZ-Binding Motif of Severe Acute Respiratory Syndrome Coronavirus Envelope Protein Is a Determinant of Viral Pathogenesis
Článek Strain-Specific Properties and T Cells Regulate the Susceptibility to Papilloma Induction by Papillomavirus 1
Článek Human Cytomegalovirus pUL79 Is an Elongation Factor of RNA Polymerase II for Viral Gene Transcription
Článek The GAP Activity of Type III Effector YopE Triggers Killing of in Macrophages
Článok vyšiel v časopise

PLOS Pathogens

2014 Číslo 8
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

Aktuální možnosti diagnostiky a léčby litiáz
nový kurz
Autori: MUDr. Tomáš Ürge, PhD.
Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#