Bub1-Mediated Adaptation of the Spindle Checkpoint
During cell division, the spindle checkpoint ensures accurate chromosome segregation by monitoring the kinetochore–microtubule interaction and delaying the onset of anaphase until each pair of sister chromosomes is properly attached to microtubules. The spindle checkpoint is deactivated as chromosomes start moving toward the spindles in anaphase, but the mechanisms by which this deactivation and adaptation to prolonged mitotic arrest occur remain obscure. Our results strongly suggest that Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest.
Published in the journal:
Bub1-Mediated Adaptation of the Spindle Checkpoint. PLoS Genet 7(1): e32767. doi:10.1371/journal.pgen.1001282
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1001282
Summary
During cell division, the spindle checkpoint ensures accurate chromosome segregation by monitoring the kinetochore–microtubule interaction and delaying the onset of anaphase until each pair of sister chromosomes is properly attached to microtubules. The spindle checkpoint is deactivated as chromosomes start moving toward the spindles in anaphase, but the mechanisms by which this deactivation and adaptation to prolonged mitotic arrest occur remain obscure. Our results strongly suggest that Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest.
Introduction
The kinetochore, composed of centromere DNA and associated proteins, mediates the attachment of chromosomes to spindle microtubules and directs chromosome movement during mitosis and meiosis, thus maintaining the high fidelity of chromosome transmission during cell division. The plus ends of microtubules are captured and stabilized by kinetochores, causing chromosomes to mono-orient to 1 pole [1]–[3]. Replicated chromosomes are composed of 2 chromatids, each with its own kinetochore. Chromosomes become bi-oriented when sister kinetochores are captured by a microtubule emanating from the opposite pole [4]–[7]. Sister chromatids remain paired until all chromosomes achieve correct bi-orientation. Sister chromatid cohesion is regulated by the control of separase activity [2], [8]–[11]. Sister chromatids disjoin after all chromosomes are bi-oriented, marking the onset of anaphase; this lack of cohesion allows each chromatid to move to its respective pole. The metaphase-to-anaphase transition and exit from mitosis are initiated by a ubiquitin-mediated proteolysis complex called the cyclosome or anaphase-promoting complex (APC/C). Before anaphase, separase is inactive because it is bound to securin [9]. Anaphase is initiated by the ubiquitin-mediated proteolysis of securin, which is triggered by activation of the APC/CCdc20 [12].
The spindle checkpoint regulates faithful chromosome segregation during mitosis by monitoring the bipolar kinetochore–microtubule interaction and delaying the onset of anaphase until stable bipolar attachment is achieved [13]. Genes involved in the spindle checkpoint were first isolated from Saccharomyces cerevisiae and include MAD1, MAD2, and MAD3 (mitotic arrest–deficient); [14] BUB1 and BUB3 (budding uninhibited by benzimidazoles [a microtubule-depolymerizing drugs]) [15]; and MPS1 (monopolar spindle) [16]. Mutual inhibition between the APC/C and Mps1, an essential component of the spindle checkpoint, causes sustained inactivation of the spindle checkpoint that cannot be reactivated in anaphase [17], and two groups have recently reported that protein phosphatase 1 activity is required for silencing the spindle checkpoint by reversing key phosphorylation events [18], [19].
The duration of mitotic arrest induced by the spindle checkpoint is not indefinite [20], [21]. Thus, cells eventually exit from mitosis and re-enter interphase. Because continued activation of the spindle checkpoint is lethal, adaptation to the spindle checkpoint arrest is beneficial so that cells have a chance to survive rather than undergo certain death [13], [22]. However, the mechanism of adaptation that could occur by spindle checkpoint inactivation remains to be characterized.
We report here that Cdc28-mediated phosphorylation of T566 plays an important role in Bub1 degradation in anaphase, and this phosphorylation is essential for deactivating the spindle checkpoint in anaphase and adaptation to prolonged mitotic arrest.
Results
Bub1 is phosphorylated at threonine-566 in a Cdc28-dependent manner in vivo
To study whether phosphorylation of Bub1 contributes to its degradation, we determined the phosphorylation sites of Bub1 by mass spectrometry. From extracts of cells expressing myc-tagged Bub1, myc-tagged Bub1 was immunoprecipitated with anti-myc antibody conjugated to Sepharose. Mass spectrometry of immunoprecipitated myc-tagged Bub1 revealed that threonine-566 (T566) on Bub1 is phosphorylated (Figure 1A).
To monitor the status of T566 phosphorylation, we generated polyclonal antibodies against an oligopeptide containing phosphorylated T566. Antibody specificity for T566 phosphorylation was verified by immunoprecipitating myc-tagged Bub1 and Bub1-T566A followed by phosphatase treatment of immunoprecipitates (Figure 1B). The band that is recognized by the phospho-T566 antibodies in wild-type cells is absent upon phosphatase treatment, confirming the specificity of phospho-T566 antibodies for phosphorylated Bub1 (Figure 1B).
We used the phospho-T566 antibodies to identify the kinase responsible for T566 phosphorylation. Sequence analysis revealed that T566 is within a potential phosphorylation site for the Cdk1/Cdc28 kinase [23]. To determine whether Cdc28 is required for T566 phosphorylation of Bub1, we analyzed wild-type strains and cdc28-1N mutants arrested in metaphase by nocodazole treatment and incubated at the nonpermissive temperature of 37°C. Phosphorylated T566 was abolished in cdc28-1N mutant cells, indicating that Cdc28 is required for T566 phosphorylation on Bub1 in vivo (Figure 1C). We also found that this phosphorylation is independent of the kinase activity of Bub1 (Figure S1). In addition, as Bub1 and Mps1 kinases act together at an early step in the spindle checkpoint pathway [24] and as Mps1 is a serine/threonine kinase [24], we also examined whether Mps1 is required for T566 phosphorylation of Bub1. However, phosphorylated T566 was not abolished in mps1-1 mutant cells at the nonpermissive temperature of 37°C, suggesting that Mps1 is not required for T566 phosphorylation on Bub1 in vivo (Figure S2).
Immunoprecipitated Cdc28 was able to efficiently phosphorylate the maltose binding protein (MBP)-fused Bub1 fragment (400–700 amino acids) but not MBP-Bub1 with T566A (400–700 amino acids), indicating that Cdc28 phosphorylates T566 on Bub1 in vitro (Figure 1D).
T566 phosphorylation plays a role in adaptation of the spindle checkpoint
Next, we examined the in vivo function of T566 phosphorylation. BUB1-T566A (this mutant is dominant, Figure S3) and bub1Δ mutant cells were sensitive to the microtubule-depolymerizing drug nocodazole (at 2.5 µg/mL and 10 µg/mL) or benomyl on a plate (Figure 2A and data not shown), suggesting that T566 phosphorylation is important for spindle checkpoint function. Thus, we examined whether BUB1-T566A mutant cells can be arrested in metaphase with nocodazole. Interestingly, a FACS analysis showed that BUB1-T566A mutant cells, but not bub1Δ mutant cells, were arrested in G2/M by nocodazole treatment (Figure 2B and Figure S4). Therefore, BUB1-T566A mutant cells can activate the spindle checkpoint in response to microtubule depolymerization.
Interestingly, FACS analysis showed that wild-type cells appeared to be “adapted” to nocodazole at a high concentration (10 µg/mL; nocodazole is nearly saturated at 10 µg/mL in water) [25] after incubation at 30°C for 5 h but BUB1-T566A mutant cells were still tightly arrested in G2/M (Figure 2B). Analysis of nuclear morphology over time revealed that adaptation to the mitotic arrest results in G1 cells (unbudded or small budded – types 1 and 2) rather than in rebudded cells without cell division (Figure 2C and 2D – type 6) and that half of these G1 cells did not have nuclei (Figure 2C and 2D – type 1), suggesting that premature cytokinesis occurred without chromosome segregation (Figure 2C and 2D). Consistent with these results, Clb2 levels started to decrease after incubation for 3 h in wild-type cells but not in BUB1-T566A mutant cells (Figure 2E). These results indicate that BUB1-T566A mutant cells were arrested in mitosis longer than wild-type cells in the presence of nocodazole at the high concentration.
We also studied the adaptation status of cells at a low concentration of nocodazole (2.5 µg/mL). At this concentration, normal cytokinesis probably occurs because the microtubules are not completely depolymerized. FACS analysis and a count of adapted cells over time suggest that there is a substantial delay in adaptation in BUB1-T566A mutant cells (Figure 3A and 3B). Time-lapse analyses revealed that BUB1-T566A mutant cells stayed longer (∼12 h) in mitosis in the presence of nocodazole at a low concentration (Figure 3C, Video S1 and S2). These results strongly suggest that BUB1-T566A mutant cells fail to adapt to the spindle checkpoint activated by nocodazole treatment after a prolonged mitotic arrest.
However, it is possible that BUB1-T566A mutant cells are sensitive to nocodazole on a plate because metaphase-arrested mutant cells cannot recover from mitotic arrest (i.e., microtubules cannot reassemble on kinetochores). To test this possibility, cells were arrested by nocodazole treatment, nocodazole was washed away, and then budded cells were counted. The increase in the number of budded BUB1-T566A mutant cells after the release was comparable to that of wild-type cells (Figure 4A), indicating that BUB1-T566A mutant cells can recover from nocodazole-induced mitotic arrest. Furthermore, in a viability assay after nocodazole incubation for several hours, BUB1-T566A mutant cells were more viable than bub1Δ mutant cells and as viable as wild-type cells (Figure 4B and Figure S5).
Therefore, we conclude that BUB1-T566A mutant cells do not grow on a nocodazole/benomyl plate because they are arrested in mitosis for a prolonged period as they cannot “adapt” and not because they die due to premature mitotic exit as in the case of bub1Δ mutant cells.
T566 phosphorylation affects Bub1 stability in mitosis
To examine the effect of phosphorylation of T566 on Bub1 stability, the HA-tagged nonphosphorylated mutant protein (T566A) was expressed from the GAL1 promoter and expression was induced for 2 h before terminating transcription by adding glucose. Indeed, the Bub1-T566A mutant protein was more stable than the wild-type protein in anaphase but not in G1 (Figure 4C and Figure S6), indicating that T566 phosphorylation is important for Bub1 degradation in anaphase.
In addition, we monitored the protein levels of myc-tagged Bub1 and Bub1-T566A mutant proteins expressed from the endogenous promoter in the presence of nocodazole at 30°C over time. FACS profiles obtained under the same conditions showed that adaptation seemed to start around 3 h after addition of nocodazole (Figure 2B). Consistently, Bub1 protein reduced significantly after 3 h of incubation with nocodazole (Figure 4D, left and Figure S7), whereas the Bub1-T566A mutant protein appeared to be more stable for 5 h incubation with nocodazole (Figure 4D, right and Figure S7).
T566A mutation does not affect kinase activity and chromosome segregation function of Bub1
Fernius and Hardwick showed that the Bub1 kinase domain is required for accurate chromosome bi-orientation after nocodazole treatment and that cells lacking the kinase domain are sensitive to microtubule drugs despite being able to arrest in response to kinetochore–microtubule attachment defects [26]. They concluded this because the kinase deletion mutants display significant chromosome missegregation when released from nocodazole arrest [26]. Therefore, we examined whether the T566A mutation affects Bub1 kinase activity and chromosome segregation function.
Immunoprecipitated myc-tagged wild-type Bub1 and Bub1-T566A proteins were used for the in vitro kinase assay, and both proteins phosphorylated histone H2A and H3 comparably (Figure 5A and Figure S8), indicating that T566A mutation does not affect kinase activity.
More importantly, BUB1-T566A mutant cells do not show a significant chromosome missegregation phenotype in colony-sectoring assays [27], [28] (Figure 5B), in diploid bimater assay (Figure 5C) [29] or in a faker assay (Figure 5D) [29]. These results indicate that T566A mutation does not affect the chromosome segregation function of Bub1. Therefore, there is no kinetochore defect that can activate the spindle checkpoint in BUB1-T566A mutant cells.
These results suggest that the stability change caused by the T566A mutation contributes to the lack-of-adaptation phenotype of BUB1-T566A mutant cells.
Discussion
We have shown that in yeast, Cdc28-mediated T566 phosphorylation of Bub1 facilitates Bub1 degradation in anaphase and thus deactivation of the spindle checkpoint in anaphase. This phosphorylation is required to adapt to prolonged mitotic arrest.
Inactivation of the spindle checkpoint in a normal cell cycle
Mps1 protein abundance decreases in anaphase and Mps1 is a target of the APC/C [17], which is a mechanism of inactivating the spindle checkpoint in anaphase. The 3-D boxes on yeast Mps1 are required for its degradation in anaphase [17], and the KEN-box on human Bub1 is required for its degradation by APC/C in mitosis [30]. However, the D-boxes or KEN-box on Bub1 are not involved in its degradation during anaphase (will be described elsewhere). T566 phosphorylation of Bub1 is important for its degradation at least in part in anaphase. Similar to findings from previous studies that levels of human Bub1 peak in mitosis and are low in G1/S phase [30], [31], we found that in the budding yeast Bub1 levels peak in G2/M and drop in late anaphase or G1 (Figure S9 and S10) as described previously [32]. Consistent with this, T566 phosphorylation peaks in G2/M (Figure S9 and S10). However, T566 phosphorylation is not essential to exit from mitosis, suggesting another mechanism to degrade Bub1. Indeed, we found that another site (amino acids 301-400) is important for Bub1 degradation in anaphase (will be described elsewhere). Also, we have not directly demonstrated that degradation of Bub1 caused by T566 phosphorylation is required for adaptation. There is a possibility that T566 phosphorylation is required for adaptation by unknown mechanisms. Further analysis is required to clarify these issues.
CDC28-VF
It has been suggested that inhibitory phosphorylation of Cdc28, which is antagonized by Cdc55 phosphatase, allows cells to exit from mitosis [22]. Also, previous studies have suggested that this “bypass” could help cells adapt to prolonged mitotic arrest [13], [22]. CDC28-T18V, Y19F (CDC28-VF), a mutant lacking inhibitory phosphorylation sites, delays the exit from mitosis and is hypersensitive to perturbations that arrest cells in mitosis [22], [33]. However, these phenotypes result not from lack of inhibitory phosphorylation or increased kinase activity but from reduced activity of the APC/C [33]. Cdc28 is required to induce metaphase-to-anaphase transition by phosphorylation of APC/C components [33], [34]. Thus, it is not certain that inhibitory phosphorylation of Cdc28 can be the mechanism of adaptation of the spindle checkpoint.
Our findings suggest that when Cdc28 activates the APC/C by phosphorylation, it simultaneously phosphorylates Bub1 to make it a preferable substrate for degradation, which in turn facilitates deactivation of the spindle checkpoint.
Nocodazole activity
To confirm that nocodazole is not deactivated after incubation for 3 h, yeast medium (YPD) containing 10 µg/mL nocodazole was incubated with yeast cells for 3 h, and fresh cycling yeast cells were incubated in the “used” medium. The FACS profiles showed that the cells were arrested in G2/M after 1.5 h and 2 h incubation, indicating that the nocodazole after incubation for 3 h was still active to arrest cells in G2/M (Figure S11). We also added more nocodazole (the total concentration was 20 µg/mL) at the 2.5 h time point, and we did not see any difference in the numbers of G1 cells at the 5 h time point with or without additional nocodazole (Figure S12). These results indicate that the premature cytokinesis is not due to inactivation of nocodazole.
Adaptation to mitotic arrest induced by the spindle checkpoint
BUB1-T566A mutant cells fail to adapt to the mitotic arrest induced by nocodazole. We found that the T566A protein is stabilized in anaphase, but the T566A mutation does not affect the kinase activity of Bub1. Therefore, the simplest interpretation is that adaptation is induced by Bub1 degradation that is regulated via T566 phosphorylation in anaphase.
If activation of the spindle checkpoint continues, cells eventually die; adaptation to the mitotic arrest gives cells a chance to survive [13], [22]. However, adaptation of the spindle checkpoint has a flip side: adapted cells could have an increased chance of aneuploidy due to premature mitotic exit.
Bub1 is functionally highly conserved between yeast and humans. The fission yeast homolog of Bub1 was shown to be phosphorylated by Cdc2 (Schizosaccharomyces pombe CDK1) in vitro, but none of the sites corresponding to T566 in budding yeast Bub1 [35]. Qi and Yu initially reported that overexpression of human BUB1-KEN box mutant protein that is stable in cells does not cause mitotic arrest or prevent cellular adaptation after prolonged nocodazole treatment [30]. However, the same group has recently found that the KEN boxes of BUB1 are required for the spindle checkpoint in human cells [36]. Also, we found that T566 is conserved in human BUB1 and BUBR1 (Figure S13). Therefore, it is still possible that this adaptation mechanism occurs in humans.
Because aneuploidy may lead to tumorigenesis in humans, either the adaptation might be controlled and balanced precisely or programmed cell death such as apoptosis might occur instead [37], [38]. Further studies of this mechanism can improve our understanding of the role of aneuploidy in the development of cancer and drug resistance of cancer cells.
Materials and Methods
Yeast strains, techniques, and media
Media and microbial and genetic techniques were used as described previously [39]. Table S1 presents the genotypes of yeast strains used for this study. Strains that expressed myc- and HA-epitope–tagged proteins were generated by the procedure of Longtine and coworkers [40]. The BUB1-T566A mutant was generated as described previously [41] by changing threonine to alanine at position 566. The mutation was verified by sequencing and integrated into the yeast genome. Integrations were amplified by PCR and fragments were sequenced to confirm the change and the strains were generated (see Table S1). To generate bub1KDΔ allele, amino acids 710–1021 were truncated by PCR-mediated gene disruption as described previously [40]. Where indicated, cells were arrested in G1 by using the alpha-factor mating pheromone (5 µg/mL; Bio Vectra, Canada). Cells were released from alpha-factor by washing twice in alpha-factor–free media and resuspended in the appropriate medium. Where indicated, the mitotic spindle was disrupted by growing cells in a medium containing nocodazole (Sigma, St. Louis, MO) and benomyl (DuPont, Wilmington, DE) at the indicated final concentration. Expression from the GAL1 promoter was induced by growing cells in media containing 2% w/v raffinose before transfer to media containing 2% galactose. Expression from the GAL1 promoter was repressed by adding 2% glucose to the medium. Cells containing the temperature-sensitive cdc15-2 allele were grown at 23°C and shifted to 37°C to induce anaphase arrest.
Plasmids
Table S2 lists the plasmids used in this study.
Mass spectrometry
The Bub1 phosphorylation site was identified by mass spectrometry performed at the Hartwell Center for Bioinformatics and Biotechnology, St. Jude Children's Research Hospital. A strain with myc epitope–tagged Bub1 was lysed, immunoprecipitated with antibody to the myc epitope, and the immunopurified Bub1 proteins were subjected to SDS-PAGE. The protein in the excised gel band was reduced and alkylated with iodoacetamide and digested with trypsin.
Digests were fractionated by online reverse-phase (C18) ultra-high-pressure liquid chromatography on a nanoAcquity Ultra Performance LC system (Waters Corporation, Milford, MA) using a Waters BEHC18 column (internal diameter of 75 µm, bed length of 10 cm, and particle size of 1.7 µm) and gradient-eluted directly into an LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, San Jose, CA) using electrospray ionization (ESI). Data dependent scanning was incorporated by acquisition of a full-scan mass spectrum followed by MS/MS on 10 most abundant precursor ions (one micro scan per spectra; precursor m/z ±1.5 Da, 35% collision energy, 30 ms ion activation, 35 s dynamic exclusion, repeat count 2).
Product ions generated by fragmentation along peptide backbone by collision activated dissociation (CAD) (b/y-type ions) were used in an automated database search against yeast BUB1 subset database using Mascot search routine with following residue modifications being allowed: Cysteine (Carbamidomethylation), Methionine (Oxidation) and Serine, Threonine and Tyrosine (Phosphorylation). Phosphopeptide identified from automated search was further validated through manual de novo sequencing of corresponding raw spectrum to assign characteristic fragment ions (b/y-type ions & ions representing loss of phosphoric acid).
Cell synchronization
For G1 arrest, the indicated strains were released from an alpha-factor-induced G1 arrest at 30°C. Samples were taken at the indicated times for FACS analysis.
For G2/M arrest, the indicated strains were tagged with the myc epitope and incubated in the presence of nocodazole at the indicated concentrations; samples were taken for FACS and Western blot analyses.
In cdc15-2 cells with HA-tagged Bub1 or Bub1-T566A expressed from the GAL1 promoter (GAL-BUB1 and GAL-BUB1-T566A) were used to test the stability of Bub1 in G1 and in anaphase. The cells were arrested in G1 (alpha-factor at 23°C) and in anaphase (cdc15-2 at 37°C). The cells were transferred to medium containing galactose (and alpha-factor where appropriate) for 2 h to induce Bub1 expression. Glucose was added to shut-off Bub1 expression; samples were taken at indicated times for Western blot analyses with antibodies to the HA epitope and tubulin protein.
To test the possibility that BUB1-T566A mutant cells might have difficulty leaving mitosis, wild-type and BUB1-T566A mutant strains were incubated with nocodazole (15 µg/mL) for 90 min at 30°C to induce mitosis and then released from nocodazole arrest; at indicated times, approximately 200 cells were counted to estimate rebudded cells (Figure 4A). All synchronizations were confirmed by FACS analysis.
FACS profile
DNA contents were measured by flow cytometry. The cell culture for each time point was washed with 0.2 M Tris-HCl (pH 7.5) and fixed in 70% ethanol at room temperature for 1 h. Cells were pelleted and resuspended in 200 µl of 0.2 M Tris-HCl (pH 7.5) containing 1 mg/mL RNase A (Sigma, St. Louis, MO). They were then incubated at 37°C for 2 h, centrifuged, resuspended in 200 µg/µL proteinase K (Invitrogen, Carlsbad, CA) for 2 h at 50°C, and stained with 3 µg/mL propidium iodide.
Microscopy
Cells were fixed with 4% paraformaldehyde for 5 min at room temperature. Cells were washed with SK buffer (1 M sorbitol, 50 mM KH2PO4 [pH 7.5]) and stained with 1 µg/mL DAPI (4′6′-diamidino-2-phenylindole) for 5 min at room temperature. The cells were washed, sonicated briefly to disrupt clumps, and mounted on glass slides. For time-lapse microscopy, cells were arrested by alpha-factor for 2 h, washed twice with alpha-factor-free media, and spotted onto glass-bottomed culture dish (MatTek, Ashland, MA) containing 2.5 µg/mL nocodazole in a YPD plate. Microscopic images were captured by using a Leica DM IRE2 motorized microscope equipped with an HCX PL FLUOT AR 40x objective lens (Leica, Germany) and images were captured with a ORCA-ER high-resolution digital CCD camera (Hamamatsu Photonics, Japan). All images were analyzed by using Openlab software (Improvision, Lexington, MA). The time-lapse analyses were conducted at 30°C.
Nocodazole and benomyl sensitivity
Sensitivity of the wild-type, bub1Δ, and BUB1-T566A mutant cells in the presence of nocodazole or benomyl was tested by growing them overnight in YPD at 30°C. Strains were then spotted in 5-fold dilutions from 4×107 cells per spot on YPD plates with and without nocodazole or benomyl.
Nocodazole survival assay
The nocodazole survival assay was performed as described previously [42]. Briefly, wild-type, bub1Δ, and BUB1-T566A mutant cells were incubated with nocodazole (15 µg/mL and 2.5 µg/mL) at the indicated times. Cells were then washed out and approximately 200 cells were plated on a YPD plate. Cell viability was calculated by dividing the number of colonies formed at the 2.5 and 5 h time points by that formed in the absence of nocodazole (0 h) at 30°C.
Sectoring assay
The colony color assay was performed as previously described [27], [43]. Briefly, wild-type, bub1Δ, and BUB1-T566A mutant cells containing a single SUP11-marked chromosome fragment were plated at a density of approximately 200 colonies per plate on minimal (SD) medium containing a limiting amount of adenine (6 µg/mL) and grown at 30°C. A colony consists of cells containing the chromosome fragment (white) and cells that have lost it (red), resulting in a white-and-red sectored phenotype.
Diploid bimater and a-Like faker assay
Bimater assay was performed as described previously [29], [44]. Diploid BUB1-T566A/BUB1-T566A mutants and haploid BUB1-T566A mutants were patched onto YPD plates. Diploid cells were replica to both MATa and MATalpha lawns and haploid cells were replica to MATalpha lawns, and mating products were selected.
Kinase assay
The kinase assay was performed as described previously [45]. Briefly, myc-tagged Bub1 and Bub1-T566A cells were immunoprecipitated with a myc epitope antibody and the immunoprecipitate was washed thrice with kinase buffer (50 mM Tris-HCl [pH 7.5], 10 mM MgCl2, and 1% Triton X-100). The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min. The reaction was stopped by adding SDS loading buffer, and the protein were then separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and analyzed by autoradiography.
Coimmunoprecipitation assay
Whole-protein extracts for Western blot analysis and immunoprecipitation assays of yeast cell lysates were performed as described previously [46].
Antibodies
Western blot analysis was performed using mouse anti-myc 9E10 purified monoclonal antibody, rat anti-HA purified monoclonal antibody (Roche Applied Science, Indianapolis, IN), and rat YOL1/34 monoclonal anti-tubulin alpha antibody (Serotec, Oxford, UK). The rabbit antibody to phosphorylated T566 of Bub1 was generated by immunizing rabbits (Covance, Denver, PA) with a corresponding KLH-conjugated phosphopeptide (H-TETDVVPIIQpTPKEQIR-OH).
Protein expression
For bacterial expression, BL21-CODONPLUS(DE3)-RIL (Stratagene, La Jolla, CA) cells were transformed with pDEST-HISMBP (Addgene, Cambridge, MA); and protein expression was performed as described previously [47].
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Štítky
Genetika Reprodukčná medicínaČlánok vyšiel v časopise
PLOS Genetics
2011 Číslo 1
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