Genetic Regulation by NLA and MicroRNA827 for Maintaining
Nitrate-Dependent Phosphate Homeostasis in
Plants need abundant nitrogen and phosphorus for higher yield. Improving plant
genetics for higher nitrogen and phosphorus use efficiency would save
potentially billions of dollars annually on fertilizers and reduce global
environmental pollution. This will require knowledge of molecular regulators for
maintaining homeostasis of these nutrients in plants. Previously, we reported
that the NITROGEN LIMITATION ADAPTATION (NLA)
gene is involved in adaptive responses to low-nitrogen conditions in
Arabidopsis, where nla mutant plants
display abrupt early senescence. To understand the molecular mechanisms
underlying NLA function, two suppressors of the
nla mutation were isolated that recover the
nla mutant phenotype to wild type. Map-based cloning
identified these suppressors as the phosphate (Pi) transport-related genes
PHF1 and PHT1.1. In addition,
NLA expression is shown to be regulated by the low-Pi
induced microRNA miR827. Pi analysis revealed that the early senescence in
nla mutant plants was due to Pi toxicity. These plants
accumulated over five times the normal Pi content in shoots specifically under
low nitrate and high Pi but not under high nitrate conditions. Also the Pi
overaccumulator pho2 mutant shows Pi toxicity in a
nitrate-dependent manner similar to the nla mutant. Further,
the nitrate and Pi levels are shown to have an antagonistic crosstalk as
displayed by their differential effects on flowering time. The results
demonstrate that NLA and miR827 have pivotal roles in
regulating Pi homeostasis in plants in a nitrate-dependent fashion.
Vyšlo v časopise:
Genetic Regulation by NLA and MicroRNA827 for Maintaining
Nitrate-Dependent Phosphate Homeostasis in. PLoS Genet 7(3): e32767. doi:10.1371/journal.pgen.1002021
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pgen.1002021
Souhrn
Plants need abundant nitrogen and phosphorus for higher yield. Improving plant
genetics for higher nitrogen and phosphorus use efficiency would save
potentially billions of dollars annually on fertilizers and reduce global
environmental pollution. This will require knowledge of molecular regulators for
maintaining homeostasis of these nutrients in plants. Previously, we reported
that the NITROGEN LIMITATION ADAPTATION (NLA)
gene is involved in adaptive responses to low-nitrogen conditions in
Arabidopsis, where nla mutant plants
display abrupt early senescence. To understand the molecular mechanisms
underlying NLA function, two suppressors of the
nla mutation were isolated that recover the
nla mutant phenotype to wild type. Map-based cloning
identified these suppressors as the phosphate (Pi) transport-related genes
PHF1 and PHT1.1. In addition,
NLA expression is shown to be regulated by the low-Pi
induced microRNA miR827. Pi analysis revealed that the early senescence in
nla mutant plants was due to Pi toxicity. These plants
accumulated over five times the normal Pi content in shoots specifically under
low nitrate and high Pi but not under high nitrate conditions. Also the Pi
overaccumulator pho2 mutant shows Pi toxicity in a
nitrate-dependent manner similar to the nla mutant. Further,
the nitrate and Pi levels are shown to have an antagonistic crosstalk as
displayed by their differential effects on flowering time. The results
demonstrate that NLA and miR827 have pivotal roles in
regulating Pi homeostasis in plants in a nitrate-dependent fashion.
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Štítky
Genetika Reprodukčná medicínaČlánok vyšiel v časopise
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