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Systematic Detection of Polygenic Regulatory
Evolution


The idea that most morphological adaptations can be attributed to changes in the

cis-regulation of gene expression levels has been gaining

increasing acceptance, despite the fact that only a handful of such cases have

so far been demonstrated. Moreover, because each of these cases involves only

one gene, we lack any understanding of how natural selection may act on

cis-regulation across entire pathways or networks. Here we

apply a genome-wide test for selection on cis-regulation to two

subspecies of the mouse Mus musculus. We find evidence for

lineage-specific selection at over 100 genes involved in diverse processes such

as growth, locomotion, and memory. These gene sets implicate candidate genes

that are supported by both quantitative trait loci and a validated

causality-testing framework, and they predict a number of phenotypic

differences, which we confirm in all four cases tested. Our results suggest that

gene expression adaptation is widespread and that these adaptations can be

highly polygenic, involving cis-regulatory changes at numerous

functionally related genes. These coordinated adaptations may contribute to

divergence in a wide range of morphological, physiological, and behavioral

phenotypes.


Vyšlo v časopise: Systematic Detection of Polygenic Regulatory Evolution. PLoS Genet 7(3): e32767. doi:10.1371/journal.pgen.1002023
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1002023

Souhrn

The idea that most morphological adaptations can be attributed to changes in the

cis-regulation of gene expression levels has been gaining

increasing acceptance, despite the fact that only a handful of such cases have

so far been demonstrated. Moreover, because each of these cases involves only

one gene, we lack any understanding of how natural selection may act on

cis-regulation across entire pathways or networks. Here we

apply a genome-wide test for selection on cis-regulation to two

subspecies of the mouse Mus musculus. We find evidence for

lineage-specific selection at over 100 genes involved in diverse processes such

as growth, locomotion, and memory. These gene sets implicate candidate genes

that are supported by both quantitative trait loci and a validated

causality-testing framework, and they predict a number of phenotypic

differences, which we confirm in all four cases tested. Our results suggest that

gene expression adaptation is widespread and that these adaptations can be

highly polygenic, involving cis-regulatory changes at numerous

functionally related genes. These coordinated adaptations may contribute to

divergence in a wide range of morphological, physiological, and behavioral

phenotypes.


Zdroje

1. Prud'hommeBGompelNCarrollSB

2007

Emerging principles of regulatory evolution.

Proc Natl Acad Sci U S A

104

Suppl 1

8605

8612

2. HoekstraHECoyneJA

2007

The locus of evolution: Evo devo and the genetics of

adaptation.

Evolution

61

995

1016

3. FraserHB

Gene expression adaptation: from single genes to genomes.

Bioessays, accepted for publication

4. WrayGA

2007

The evolutionary significance of cis-regulatory

mutations.

Nat Rev Genet

8

206

5. SternDLOrgogozoV

2008

The loci of evolution: how predictable is genetic

evolution?

Evolution

62

2155

6. PollardKSSalamaSRLambertNLambotMACoppensS

2006

An RNA gene expressed during cortical development evolved rapidly

in humans.

Nature

443

167

172

7. HaygoodRFedrigoOHansonBYokoyamaKDWrayGA

2007

Promoter regions of many neural- and nutrition-related genes have

experienced positive selection during human evolution.

Nat Genet

39

1140

1144

8. PrabhakarSNoonanJPPääboSRubinEM

2006

Accelerated evolution of conserved noncoding sequences in

humans.

Science

314

786

9. AndolfattoP

2005

Adaptive evolution of non-coding DNA in

Drosophila.

Nature

437

1149

1152

10. GaltierNDuretL

2007

Adaptation or biased gene conversion? Extending the null

hypothesis of molecular evolution.

Trends Genet

23

273

277

11. FayJCWittkoppPJ

2008

Evaluating the role of natural selection in the evolution of gene

regulation.

Heredity

100

191

199

12. OleksiakMFChurchillGACrawfordDL

2002

Variation in gene expression within and among natural

populations.

Nat Genet

32

261

13. BlekhmanROshlackAChabotAESmythGKGiladY

2008

Gene regulation in primates evolves under tissue-specific

selection pressures.

PLoS Genet

4

e1000271

doi:10.1371/journal.pgen.1000271

14. GiladYOshlackASmythGKSpeedTPWhiteKP

2006

Expression profiling in primates reveals a rapid evolution of

human transcription factors.

Nature

440

242

15. DenverDRMorrisKStreelmanJTKimSKLynchM

2005

The transcriptional consequences of mutation and natural

selection in Caenorhabditis elegans.

Nat Genet

37

544

16. RifkinSAHouleDKimJWhiteKP

2005

A mutation accumulation assay reveals a broad capacity for rapid

evolution of gene expression.

Nature

438

220

17. LandryCRLemosBRifkinSADickinsonWJHartlDL

2007

Genetic properties influencing the evolvability of gene

expression.

Science

317

118

18. FraserHBMosesASchadtEE

2010

Evidence for widespread adaptive evolution of gene expression in

budding yeast.

Proc Natl Acad Sci U S A

107

2977

2982

19. BullardJHMostovoyYDudoitSBremRB

2010

Polygenic and directional regulatory evolution across pathways in

Saccharomyces.

Proc Natl Acad Sci U S A

107

5058

5063

20. GibsonGWeirB

2005

The quantitative genetics of transcription.

Trends Genet

21

616

623

21. SteinerCCWeberJNHoekstraHE

2007

Adaptive variation in beach mice produced by two interacting

pigmentation genes.

PLoS Biol

5

e219

doi:10.1371/journal.pbio.0050219

22. OrrHA

1998

Testing natural selection versus genetic drift in phenotypic

evolution using quantitative trait locus data.

Genetics

149

2099

2104

23. DossSSchadtEEDrakeTALusisAJ

2005

Cis-acting expression quantitative trait loci in

mice.

Genome Res

15

681

691

24. McDonaldJHKreitmanM

1991

Adaptive protein evolution at the Adh locus in

Drosophila.

Nature

351

652

25. HughesAL

2007

Looking for Darwin in all the wrong places: the misguided quest

for positive selection at the nucleotide sequence level.

Heredity

99

364

373

26. FrazerKAEskinEKangHMBogueMAHindsDA

2007

A sequence-based variation map of 8.27 million SNPs in inbred

mouse strains.

Nature

448

1050

1053

27. GeraldesABassetPGibsonBSmithKLHarrB

2008

Inferring the history of speciation in house mice from autosomal,

X-linked, Y-linked and mitochondrial genes.

Mol Ecol

17

5349

5363

28. The Gene Ontology Consortium

2000

Gene Ontology: tool for the unification of

biology.

Nat Genet

25

25

29

29. KanehisaMGotoSHattoriMAoki-KinoshitaKFItohM

2006

From genomics to chemical genomics: new developments in

KEGG.

Nucleic Acids Res

34

D354

D357

30. BabakTDevealeBArmourCRaymondCClearyMA

2008

Global survey of genomic imprinting by transcriptome

sequencing.

Curr Biol

18

1735

1741

31. WangXSunQMcGrathSDMardisERSolowayPD

2008

Transcriptome-wide identification of novel imprinted genes in

neonatal mouse brain.

PLoS ONE

3

e3839

doi:10.1371/journal.pone.0003839

32. BabakT

2010

Genetic validation of whole-transcriptome sequencing for mapping

expression affected by cis-regulatory

variation.

BMC Genomics

11

473

33. BabakTGarrett-EngelePArmourCDRaymondCKKellerMP

2009

The Mouse Genome Database genotypes::phenotypes.

Nucl Acids Res

37

D712

D719

34. BouwmanPGöllnerHElsässerHPEckhoffGKarisA

2000

Transcription factor Sp3 is essential for post-natal survival and

late tooth development.

EMBO J

19

655

661

35. SimmerFMoormanCvan der LindenAMKuijkEvan den BerghePV

2003

Genome-wide RNAi of C. elegans using the

hypersensitive rrf-3 strain reveals novel gene

functions.

PLoS Biol

1

e12

doi:10.1371/journal.pbio.0000012

36. SchadtEELambJYangXZhuJEdwardsS

2005

An integrative genomics approach to infer causal associations

between gene expression and disease.

Nat Genet

37

710

717

37. MillsteinJZhangBZhuJSchadtEE

2009

Disentangling molecular relationships with a causal inference

test.

BMC Genet

10

23

38. YangXDeignanJLQiHZhuJQianS

2009

Validation of candidate causal genes for obesity that affect

shared metabolic pathways and networks.

Nat Genet

41

415

423

39. BachmanovAAReedDRBeauchampGKTordoffMG

2002

Food intake, water intake, and drinking spout side preference of

28 mouse strains.

Behav Genet

32

435

443

40. Le RoyIRoubertouxPLJamotLMaaroufFTordjmanS

1998

Neuronal and behavioral differences between Mus musculus

domesticus (C57BL/6JBy) and Mus musculus

castaneus (CAST/Ei).

Behav Brain Res

95

135

142

41. KoideTMoriwakiKIkedaKNikiHShiroishiT

2000

Multi-phenotype behavioral characterization of inbred strains

derived from wild stocks of Mus musculus.

Mamm Genome

11

664

670

42. BrownREWongAA

2007

The influence of visual ability on learning and memory

performance in 13 strains of mice.

Learn Mem

14

134

144

43. SakaiTKikkawaYMiuraIInoueTMoriwakiK

2005

Origins of mouse inbred strains deduced from whole-genome

scanning by polymorphic microsatellite loci.

Mamm Genome

16

11

19

44. SchlosserG

2002

Modularity and the units of evolution.

Theory in Biosciences

121

1

80

45. GrazeRMMcIntyreLMMainBJWayneMLNuzhdinSV

2009

Regulatory divergence in Drosophila melanogaster

and D. simulans, a genomewide analysis of allele-specific

expression.

Genetics

183

547

561

46. ZhangXBorevitzJO

2009

Global analysis of allele-specific expression in

Arabidopsis thaliana.

Genetics

182

943

954

47. GompelNPrud'hommeBWittkoppPJKassnerVACarrollSB

2005

Chance caught on the wing: cis-regulatory evolution and the

origin of pigment patterns in Drosophila.

Nature

433

481

487

48. MillerCTBelezaSPollenAASchluterDKittlesRA

2007

cis-Regulatory changes in Kit ligand expression

and parallel evolution of pigmentation in sticklebacks and

humans.

Cell

131

1179

1189

49. ShapiroMDMarksMEPeichelCLBlackmanBKNerengKS

2004

Genetic and developmental basis of evolutionary pelvic reduction

in threespine sticklebacks.

Nature

428

717

723

50. HaygoodRBabbittCCFedrigoOWrayGA

2010

Contrasts between adaptive coding and noncoding changes during

human evolution.

Proc Natl Acad Sci U S A

107

7853

7857

51. LiaoBYWengMPZhangJ

2010

Contrasting genetic paths to morphological and physiological

evolution.

Proc Natl Acad Sci U S A

107

7353

7358

52. HeYDDaiHSchadtEECavetGEdwardsSW

2003

Microarray standard data set and figures of merit for comparing

data processing methods and experiment designs.

Bioinformatics

19

956

965

53. ZhuJLumPYLambJGuhaThakurtaDEdwardsSW

2004

An integrative genomics approach to the reconstruction of gene

networks in segregating populations. Cytogenet.

Genome Res

105

363

374

Štítky
Genetika Reprodukčná medicína

Článok vyšiel v časopise

PLOS Genetics


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