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Quantitative Comparison of HTLV-1 and HIV-1 Cell-to-Cell Infection with New Replication Dependent Vectors


We have developed an efficient method to quantify cell-to-cell infection with single-cycle, replication dependent reporter vectors. This system was used to examine the mechanisms of infection with HTLV-1 and HIV-1 vectors in lymphocyte cell lines. Effector cells transfected with reporter vector, packaging vector, and Env expression plasmid produced virus-like particles that transduced reporter gene activity into cocultured target cells with zero background. Reporter gene expression was detected exclusively in target cells and required an Env-expression plasmid and a viral packaging vector, which provided essential structural and enzymatic proteins for virus replication. Cell-cell fusion did not contribute to infection, as reporter protein was rarely detected in syncytia. Coculture of transfected Jurkat T cells and target Raji/CD4 B cells enhanced HIV-1 infection two fold and HTLV-1 infection ten thousand fold in comparison with cell-free infection of Raji/CD4 cells. Agents that interfere with actin and tubulin polymerization strongly inhibited HTLV-1 and modestly decreased HIV-1 cell-to-cell infection, an indication that cytoskeletal remodeling was more important for HTLV-1 transmission. Time course studies showed that HTLV-1 transmission occurred very rapidly after cell mixing, whereas slower kinetics of HIV-1 coculture infection implies a different mechanism of infectious transmission. HTLV-1 Tax was demonstrated to play an important role in altering cell-cell interactions that enhance virus infection and replication. Interestingly, superantigen-induced synapses between Jurkat cells and Raji/CD4 cells did not enhance infection for either HTLV-1 or HIV-1. In general, the dependence on cell-to-cell infection was determined by the virus, the effector and target cell types, and by the nature of the cell-cell interaction.


Vyšlo v časopise: Quantitative Comparison of HTLV-1 and HIV-1 Cell-to-Cell Infection with New Replication Dependent Vectors. PLoS Pathog 6(2): e32767. doi:10.1371/journal.ppat.1000788
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1000788

Souhrn

We have developed an efficient method to quantify cell-to-cell infection with single-cycle, replication dependent reporter vectors. This system was used to examine the mechanisms of infection with HTLV-1 and HIV-1 vectors in lymphocyte cell lines. Effector cells transfected with reporter vector, packaging vector, and Env expression plasmid produced virus-like particles that transduced reporter gene activity into cocultured target cells with zero background. Reporter gene expression was detected exclusively in target cells and required an Env-expression plasmid and a viral packaging vector, which provided essential structural and enzymatic proteins for virus replication. Cell-cell fusion did not contribute to infection, as reporter protein was rarely detected in syncytia. Coculture of transfected Jurkat T cells and target Raji/CD4 B cells enhanced HIV-1 infection two fold and HTLV-1 infection ten thousand fold in comparison with cell-free infection of Raji/CD4 cells. Agents that interfere with actin and tubulin polymerization strongly inhibited HTLV-1 and modestly decreased HIV-1 cell-to-cell infection, an indication that cytoskeletal remodeling was more important for HTLV-1 transmission. Time course studies showed that HTLV-1 transmission occurred very rapidly after cell mixing, whereas slower kinetics of HIV-1 coculture infection implies a different mechanism of infectious transmission. HTLV-1 Tax was demonstrated to play an important role in altering cell-cell interactions that enhance virus infection and replication. Interestingly, superantigen-induced synapses between Jurkat cells and Raji/CD4 cells did not enhance infection for either HTLV-1 or HIV-1. In general, the dependence on cell-to-cell infection was determined by the virus, the effector and target cell types, and by the nature of the cell-cell interaction.


Zdroje

1. JohnsonDC

HuberMT

2002 Directed egress of animal viruses promotes cell-to-cell spread. J Virol 76 1 8

2. JollyC

KashefiK

HollinsheadM

SattentauQJ

2004 HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse. J Exp Med 199 283 293

3. MarshM

HeleniusA

2006 Virus entry: open sesame. Cell 124 729 740

4. PhillipsDM

1994 The role of cell-to-cell transmission in HIV infection. Aids 8 719 731

5. PiguetV

SattentauQ

2004 Dangerous liaisons at the virological synapse. J Clin Invest 114 605 610

6. SatoH

OrensteinJ

DimitrovD

MartinM

1992 Cell-to-cell spread of HIV-1 occurs within minutes and may not involve the participation of virus particles. Virology 186 712 724

7. JollyC

MitarI

SattentauQJ

2007 Requirement for an intact T-cell actin and tubulin cytoskeleton for efficient assembly and spread of human immunodeficiency virus type 1. J Virol 81 5547 5560

8. JollyC

SattentauQJ

2004 Retroviral spread by induction of virological synapses. Traffic 5 643 650

9. Pearce-PrattR

MalamudD

PhillipsDM

1994 Role of the cytoskeleton in cell-to-cell transmission of human immunodeficiency virus. J Virol 68 2898 2905

10. ChenP

HubnerW

SpinelliMA

ChenBK

2007 Predominant mode of human immunodeficiency virus transfer between T cells is mediated by sustained Env-dependent neutralization-resistant virological synapses. J Virol 81 12582 12595

11. Sol-FoulonN

SourisseauM

PorrotF

ThoulouzeMI

TrouilletC

2007 ZAP-70 kinase regulates HIV cell-to-cell spread and virological synapse formation. Embo J 26 516 526

12. SourisseauM

Sol-FoulonN

PorrotF

BlanchetF

SchwartzO

2007 Inefficient human immunodeficiency virus replication in mobile lymphocytes. J Virol 81 1000 1012

13. MatsuokaM

JeangKT

2007 Human T-cell leukaemia virus type 1 (HTLV-1) infectivity and cellular transformation. Nat Rev Cancer 7 270 280

14. IgakuraT

StinchcombeJC

GoonPK

TaylorGP

WeberJN

2003 Spread of HTLV-I between lymphocytes by virus-induced polarization of the cytoskeleton. Science 299 1713 1716

15. MajorovitsE

NejmeddineM

TanakaY

TaylorGP

FullerSD

2008 Human T-lymphotropic virus-1 visualized at the virological synapse by electron tomography. PLoS ONE 3 e2251 doi:10.1371/journal.pone.0002251

16. AlfsenA

YuH

Magerus-ChatinetA

SchmittA

BomselM

2005 HIV-1-infected blood mononuclear cells form an integrin- and agrin-dependent viral synapse to induce efficient HIV-1 transcytosis across epithelial cell monolayer. Mol Biol Cell 16 4267 4279

17. CarrJM

HockingH

LiP

BurrellCJ

1999 Rapid and efficient cell-to-cell transmission of human immunodeficiency virus infection from monocyte-derived macrophages to peripheral blood lymphocytes. Virology 265 319 329

18. PhillipsDM

BourinbaiarAS

1992 Mechanism of HIV spread from lymphocytes to epithelia. Virology 186 261 273

19. GeijtenbeekTB

KwonDS

TorensmaR

van VlietSJ

van DuijnhovenGC

2000 DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 100 587 597

20. GummuluruS

KewalRamaniVN

EmermanM

2002 Dendritic cell-mediated viral transfer to T cells is required for human immunodeficiency virus type 1 persistence in the face of rapid cell turnover. J Virol 76 10692 10701

21. McDonaldD

WuL

BohksSM

KewalRamaniVN

UnutmazD

2003 Recruitment of HIV and its receptors to dendritic cell-T cell junctions. Science 300 1295 1297

22. ShererNM

LehmannMJ

Jimenez-SotoLF

HorensavitzC

PypaertM

2007 Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission. Nat Cell Biol 9 310 315

23. RudnickaD

FeldmannJ

PorrotF

WietgrefeS

GuadagniniS

2009 Simultaneous cell-to-cell transmission of human immunodeficiency virus to multiple targets through polysynapses. J Virol 83 6234 6246

24. JinJ

ShererNM

HeideckerG

DerseD

MothesW

2009 Assembly of the murine leukemia virus is directed towards sites of cell-cell contact. PLoS Biol 7 e1000163 doi:10.1371/journal.pbio.1000163

25. DavisAJ

LiP

BurrellCJ

1997 Kinetics of viral RNA synthesis following cell-to-cell transmission of human immunodeficiency virus type 1. J Gen Virol 78 (Pt 8) 1897 1906

26. HubnerW

McNerneyGP

ChenP

DaleBM

GordonRE

2009 Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science 323 1743 1747

27. HeidmannT

HeidmannO

NicolasJF

1988 An indicator gene to demonstrate intracellular transposition of defective retroviruses. Proc Natl Acad Sci U S A 85 2219 2223

28. HeidmannO

HeidmannT

1991 Retrotransposition of a mouse IAP sequence tagged with an indicator gene. Cell 64 159 170

29. MoranJV

HolmesSE

NaasTP

DeBerardinisRJ

BoekeJD

1996 High frequency retrotransposition in cultured mammalian cells. Cell 87 917 927

30. CurcioMJ

GarfinkelDJ

1991 Single-step selection for Ty1 element retrotransposition. Proc Natl Acad Sci U S A 88 936 940

31. WangJH

JanasAM

OlsonWJ

KewalRamaniVN

WuL

2007 CD4 coexpression regulates DC-SIGN-mediated transmission of human immunodeficiency virus type 1. J Virol 81 2497 2507

32. GhezD

LepelletierY

LambertS

FourneauJM

BlotV

2006 Neuropilin-1 is involved in human T-cell lymphotropic virus type 1 entry. J Virol 80 6844 6854

33. JonesKS

Petrow-SadowskiC

BertoletteDC

HuangY

RuscettiFW

2005 Heparan sulfate proteoglycans mediate attachment and entry of human T-cell leukemia virus type 1 virions into CD4+ T cells. J Virol 79 12692 12702

34. ManelN

KimFJ

KinetS

TaylorN

SitbonM

2003 The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV. Cell 115 449 459

35. LongoDL

GelmannEP

CossmanJ

YoungRA

GalloRC

1984 Isolation of HTLV-transformed B-lymphocyte clone from a patient with HTLV-associated adult T-cell leukaemia. Nature 310 505 506

36. MannDL

ClarkJ

ClarkeM

ReitzM

PopovicM

1984 Identification of the human T cell lymphoma virus in B cell lines established from patients with adult T cell leukemia. J Clin Invest 74 56 62

37. OkadaM

KoyanagiY

KobayashiN

TanakaY

NakaiM

1984 In vitro infection of human B lymphocytes with adult T-cell leukemia virus. Cancer Lett 22 11 21

38. UedaS

MaedaY

YamaguchiT

HanamotoH

HijikataY

2008 Influence of Epstein-Barr virus infection in adult T-cell leukemia. Hematology 13 154 162

39. YamamotoN

MatsumotoT

KoyanagiY

TanakaY

HinumaY

1982 Unique cell lines harbouring both Epstein-Barr virus and adult T-cell leukaemia virus, established from leukaemia patients. Nature 299 367 369

40. MazurovD

HeideckerG

DerseD

2006 HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane. Virology 346 194 204

41. SanchoD

MontoyaMC

MonjasA

Gordon-AlonsoM

KatagiriT

2002 TCR engagement induces proline-rich tyrosine kinase-2 (Pyk2) translocation to the T cell-APC interface independently of Pyk2 activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion. J Immunol 169 292 300

42. DerseD

HillSA

LloydPA

ChungH

MorseBA

2001 Examining human T-lymphotropic virus type 1 infection and replication by cell-free infection with recombinant virus vectors. J Virol 75 8461 8468

43. DerseD

MikovitsJ

WatersD

BriningS

RF

1996 Examining the Molecular Genetics of HTLV-1 with an Infectious Molecular Clone of the Virus and Permissive Cell Culture Systems. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 12 1 5

44. GiamCZ

JeangKT

2007 HTLV-1 Tax and adult T-cell leukemia. Front Biosci 12 1496 1507

45. TakahashiT

HiguchiM

FukushiM

OieM

ItoM

2002 Homotypic cell-cell adhesion induced by human T cell leukemia virus type 1 tax protein in T cell lines. Virology 302 132 143

46. MorganMM

LabnoCM

Van SeventerGA

DennyMF

StrausDB

2001 Superantigen-induced T cell:B cell conjugation is mediated by LFA-1 and requires signaling through Lck, but not ZAP-70. J Immunol 167 5708 5718

47. MiyauchiK

KimY

LatinovicO

MorozovV

MelikyanGB

2009 HIV enters cells via endocytosis and dynamin-dependent fusion with endosomes. Cell 137 433 444

48. RayneF

KajavaAV

LalanneJ

MamounRZ

2004 In vivo homodimerisation of HTLV-1 Gag and MA gives clues to the retroviral capsid and TM envelope protein arrangement. J Mol Biol 343 903 916

49. MazurovD

HeideckerG

DerseD

2007 The inner loop of tetraspanins CD82 and CD81 mediates interactions with human T cell lymphotrophic virus type 1 Gag protein. J Biol Chem 282 3896 3903

50. GaneshL

LeungK

LoreK

LevinR

PanetA

2004 Infection of specific dendritic cells by CCR5-tropic human immunodeficiency virus type 1 promotes cell-mediated transmission of virus resistant to broadly neutralizing antibodies. J Virol 78 11980 11987

51. NejmeddineM

BarnardAL

TanakaY

TaylorGP

BanghamCR

2005 Human T-lymphotropic virus, type 1, tax protein triggers microtubule reorientation in the virological synapse. J Biol Chem 280 29653 29660

52. TanakaY

FukudomeK

HayashiM

TakagiS

YoshieO

1995 Induction of ICAM-1 and LFA-3 by Tax1 of human T-cell leukemia virus type 1 and mechanism of down-regulation of ICAM-1 or LFA-1 in adult-T-cell-leukemia cell lines. Int J Cancer 60 554 561

53. ChungHK

YoungHA

GoonPK

HeideckerG

PrinclerGL

2003 Activation of interleukin-13 expression in T cells from HTLV-1-infected individuals and in chronically infected cell lines. Blood 102 4130 4136

54. ChesebroB

WehrlyK

1988 Development of a sensitive quantitative focal assay for human immunodeficiency virus infectivity. J Virol 62 3779 3788

55. NaldiniL

BlomerU

GageFH

TronoD

VermaIM

1996 Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci U S A 93 11382 11388

56. MurakamiT

FreedEO

2000 The long cytoplasmic tail of gp41 is required in a cell type-dependent manner for HIV-1 envelope glycoprotein incorporation into virions. Proc Natl Acad Sci U S A 97 343 348

57. ShuhM

DerseD

2000 Ternary complex factors and cofactors are essential for human T-cell leukemia virus type 1 tax transactivation of the serum response element. J Virol 74 11394 11397

58. BishopN

WoodmanP

2000 ATPase-defective mammalian VPS4 localizes to aberrant endosomes and impairs cholesterol trafficking. Mol Biol Cell 11 227 239

59. DerseD

HillSA

PrinclerG

LloydP

HeideckerG

2007 Resistance of human T cell leukemia virus type 1 to APOBEC3G restriction is mediated by elements in nucleocapsid. Proc Nat Acad Sci U S A 104 2915 2920

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Hygiena a epidemiológia Infekčné lekárstvo Laboratórium

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