The Hypervariable Amino-Terminus of P1 Protease Modulates Potyviral Replication and Host Defense Responses
RNA viruses are ideal systems for the study of population dynamics, relationships among pathogen traits such as fitness and virulence, and of host immune responses to pathogen attacks. Based on experimental evolution studies, early models equated parasite virulence with fitness. Some reports showed that viral virulence and fitness can be unlinked. Here we present evidence that the highly disordered N-terminal region of a potyviral P1 protein negatively regulates its self-cleavage activity. Removal of this regulator domain greatly affects viral infection, which is characterized by accelerated early replication and enhanced symptom severity. These properties are nonetheless associated with low viral accumulation and high induction of antiviral resistance markers. Finally, we propose that host-dependent regulation of P1 processing efficiency modulates viral virulence and alleviates the host antiviral responses.
Vyšlo v časopise:
The Hypervariable Amino-Terminus of P1 Protease Modulates Potyviral Replication and Host Defense Responses. PLoS Pathog 10(3): e32767. doi:10.1371/journal.ppat.1003985
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1003985
Souhrn
RNA viruses are ideal systems for the study of population dynamics, relationships among pathogen traits such as fitness and virulence, and of host immune responses to pathogen attacks. Based on experimental evolution studies, early models equated parasite virulence with fitness. Some reports showed that viral virulence and fitness can be unlinked. Here we present evidence that the highly disordered N-terminal region of a potyviral P1 protein negatively regulates its self-cleavage activity. Removal of this regulator domain greatly affects viral infection, which is characterized by accelerated early replication and enhanced symptom severity. These properties are nonetheless associated with low viral accumulation and high induction of antiviral resistance markers. Finally, we propose that host-dependent regulation of P1 processing efficiency modulates viral virulence and alleviates the host antiviral responses.
Zdroje
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Štítky
Hygiena a epidemiológia Infekčné lekárstvo LaboratóriumČlánok vyšiel v časopise
PLOS Pathogens
2014 Číslo 3
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- Parazitičtí červi v terapii Crohnovy choroby a dalších zánětlivých autoimunitních onemocnění
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