Combined Systems Approaches Reveal Highly Plastic Responses to Antimicrobial Peptide Challenge in

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Antimicrobial peptides (AMP) are small proteins with often potent antibacterial activity found in a variety of organisms, including humans. Understanding how these antibiotics operate is challenging and often controversial since many studies have necessarily focussed on identifying a single major cause of bacterial cell death while, increasingly, others have cautioned that AMPs are likely to have access to multiple bactericidal features. Systems biology is an emerging field that comprises a series of techniques capable of giving a global view of how bacteria respond to external stimuli. Here we have monitored changes in gene expression and metabolism in bacteria that have been challenged with sub-lethal concentrations of four different AMPs. By understanding how bacteria respond to a threat we can reveal how the bacteria perceive the AMP to be operating. Our approach provides a sophisticated bacterial perspective of the mode of action of each AMP and reveals that the bacteria have a vast array of weapons that can be marshalled to deal with distinct AMP threats. Indeed, around a third (or even more) of the bacterial machinery might be useful in dealing with antibiotic challenges, highlighting why antibiotic resistance is such a persistent problem.

Vyšlo v časopise: Combined Systems Approaches Reveal Highly Plastic Responses to Antimicrobial Peptide Challenge in. PLoS Pathog 10(5): e32767. doi:10.1371/journal.ppat.1004104
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004104

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