Genome-Wide Identification of the Target Genes of AP2-O, a AP2-Family Transcription Factor

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Although malarial parasites have a complex life cycle, they harbor only 30 transcription factors in their genome. The majority of these transcription factors belong to a single family referred to as the AP2 family. Our previous study suggested that stage-specific AP2 family transcription factors have critical roles in maintaining the Plasmodium parasite life cycle. However, it remains fairly elusive as to how these transcription factors regulate each stage. AP2-O is an AP2 family transcription factor that is expressed during the mosquito midgut-invading stage, the ookinete, and is essential for normal development of this stage. In the present study, we identified the entire set of AP2-O target genes to elucidate how this AP2 family transcription factor contributes to the formation of this stage. Our results showed that AP2-O directly regulates 10% of the parasite genome and is involved in the whole process of mosquito midgut-invasion by ookinetes. The global and comprehensive regulation by the AP2 family transcription factor that we revealed provides a model for transcriptional regulation of this parasite and may explain how malarial parasites regulate their complex life cycle using a small number of sequence-specific transcription factors.

Vyšlo v časopise: Genome-Wide Identification of the Target Genes of AP2-O, a AP2-Family Transcription Factor. PLoS Pathog 11(5): e32767. doi:10.1371/journal.ppat.1004905
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004905

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