Analysis of Protein Using Mass Spectrometry

Česká verzia

Authors: P. Dvořáková; L. Hernychová; B. Vojtěšek
Authors place of work: Regionální centrum aplikované molekulární onkologie, Masarykův onkologický ústav, Brno
Published in the journal: Klin Onkol 2014; 27(Supplementum): 104-109

Summary

Recently, mass spectrometry has become a powerful tool in cancer research. Mass spectrometry represents the method that allows identification, quantification and characterization of proteins in biological samples. Nowadays, it is mainly used for biomarker discovery that can enable early detection of cancer. This article is focused on protein analysis by mass spectrometry. At first, mass spectrometry and its importance in proteomics are described. Subsequently ionization type and mass analyzers are discussed. This relates to the possibility of on‑line or off‑line analysis connection with separation techniques, such as liquid chromatography and electrophoresis. Different approaches for preparing proteins and methods of analysis of biomolecules using mass spectrometers are described. In addition, the possibility of mass spectrometric analyses of samples and data processing are discussed.

Key words:
mass spectrometry – liquid chromatography – electrophoresis – proteomics

This work was supported by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101) and by MH CZ – DRO (MMCI, 00209805).

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Submitted:
31. 1. 2014

Accepted:
25. 3. 2014

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