New Trends in the Study of Protein Glycosylation in Oncological Diseases

Česká verzia

Authors: M. Zahradníková; L. Hernychová; B. Vojtěšek; M. V. Novotný
Authors place of work: Regionální centrum aplikované molekulární onkologie, Masarykův onkologický ústav, Brno
Published in the journal: Klin Onkol 2014; 27(Supplementum): 121-128

Summary

Glycomics and glycoproteomics represent relatively new directions in detail analyses of complex biological media. These areas of increasing importance to cancer research complement the more established genomic profiling and proteomics. Glycoproteins are being increasingly recognized as important in cellular interactions and adhesion. Structural alterations of their glycan moieties seem to occur in different cancer conditions. We review current directions in glycomic profiling and glycoproteomic investigations of biological fluids and tissues pertaining to cancer. The used methods rely on capillary separation techniques, mass spectrometry, and the glycan and lectin arrays. They all show considerable promise for new diagnostic and prognostic measurements.

Key words:
glycomics – glycopeptides – cancer – liquid chromatography – mass spectrometry – capillary electrophoresis – glycan profiling – array analysis

This work was supported by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101) and by MH CZ – DRO (MMCI, 00209805).

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Submitted:
21. 3. 2014

Accepted:
10. 4. 2014

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