An Intronic microRNA Links Rb/E2F and EGFR Signaling

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Animal genomes encode hundreds of microRNA genes that impact all areas of biology by limiting the expression of their targets. What remains largely unappreciated is that a significant proportion of microRNA genes are embedded within protein-coding genes, and are often co-expressed with their hosts, which raises the possibility of a functional interaction between them. The mir-998 gene is located within an intron of the gene encoding Drosophila E2F1 transcription factor. E2F1 can induce the expression of cell death genes, and its activity is negatively regulated by the pRB tumour suppressor protein. In certain settings, unrestrained E2F1 activity is sufficient to induce cell death in cells lacking functional pRB. Here, we show that miR-998 limits cell death in Rb-deficient cells by repressing dCbl, a negative regulator of Epidermal Growth Factor Receptor signaling (EGFR). miR-998 also augments EGFR signaling in differentiating photoreceptor cells. Furthermore, we show that the interaction between miR-998 and Cbl is conserved: in human cells, miR-29, a mir-29/998 seed family member, enhances EGFR signaling by targeting c-Cbl. Therefore, by examining the role of an intronic microRNA in the context of its host's function, we identified an important microRNA target and uncovered a biological function of the microRNA.

Vyšlo v časopise: An Intronic microRNA Links Rb/E2F and EGFR Signaling. PLoS Genet 10(7): e32767. doi:10.1371/journal.pgen.1004493
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004493

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