Genetic Deletion of SEPT7 Reveals a Cell Type-Specific Role of Septins in Microtubule Destabilization for the Completion of Cytokinesis

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Cytokinesis is the finalizing step of the complex scenario of mitosis, leading to separation of two sister cells. The cellular mechanism of cytokinesis in eukaryotes differs at least between yeasts, plants and animals. So far, it is also not clear whether all mammalian cells follow the same mechanistic rules of cytokinesis. Here, we demonstrate that, depending on the mammalian cell type, two different pathways could result in completion of cytokinesis, a septin-dependent pathway and a distinct mechanism, which does not require septins prevalent in the hematopoietic system. Using multiple conditional knockouts, we demonstrate this cell type specificity in vitro and in vivo, and present evidence for the involvement of cell-type specific alteration of the microtubule cytoskeleton. Our data, together with the previously available septin knockdown data in cancer cell lines, suggest septins as plausible antitumor targets with high therapeutic index due to lack of off-target effects on hematopoiesis.

Vyšlo v časopise: Genetic Deletion of SEPT7 Reveals a Cell Type-Specific Role of Septins in Microtubule Destabilization for the Completion of Cytokinesis. PLoS Genet 10(8): e32767. doi:10.1371/journal.pgen.1004558
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004558

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