Roles of Type 1A Topoisomerases in Genome Maintenance in

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DNA topoisomerases are ubiquitous enzymes that solve the topological problems associated with replication, transcription and recombination. Eukaryotic enzymes of the type 1A family work with RecQ-like helicases such as BLM and Sgs1 and are involved in genome maintenance. Interestingly, E. coli topo I, a type 1A enzyme and the first topoisomerase to be discovered, appears to have distinct cellular functions that are related to supercoiling regulation and to the inhibition of R-loop formation. Here we present data strongly suggesting that these cellular functions are required to inhibit inappropriate replication originating from either oriC, the normal origin of replication, or R-loops that can otherwise lead to severe chromosome segregation defects. Avoiding such inappropriate replication appears to be a key cellular function for genome maintenance, since the other E. coli type 1A topo, topo III, is also involved. Furthermore, our data suggest that bacterial type 1A topos, like their eukaryotic counterparts, can act with RecQ in genome maintenance. Altogether, our data provide new insight into the role of type 1A topos in genome maintenance and reveal an interplay between these enzymes and R-loops, structures that can also significantly affect the stability of the genome as recently shown in numerous studies.

Vyšlo v časopise: Roles of Type 1A Topoisomerases in Genome Maintenance in. PLoS Genet 10(8): e32767. doi:10.1371/journal.pgen.1004543
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004543

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