VIB1, a Link between Glucose Signaling and Carbon Catabolite Repression, Is Essential for Plant Cell Wall Degradation by

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Many filamentous fungi that grow on plant biomass are capable of producing lignocellulase enzymes to break down plant cell walls into utilizable sugars, thus holding great potential in reducing the cost of the next-generation biofuels. Cellulase production is subject to induction by the presence of plant biomass components and to repression by the availability of easily metabolized sugars, such as glucose. Genes required for repression of cellulase gene expression when preferred carbon sources are present (carbon catabolite repression) and those that play a role in mediating glucose sensing/metabolism have been identified in filamentous fungi, but the mechanisms involved in crosstalk between repression versus induction of cellulase gene expression is poorly understood. Here, we report the identification and functional characterization of VIB1, a transcription factor essential for plant cell wall deconstruction in Neurospora crassa and COL26, a transcription factor that functions in glucose sensing/metabolism and regulation of CCR. We show that disabling CRE1 repression and modulating the glucose response by deletion of col-26 restored growth of the Δvib-1 mutant on cellulose. Our findings are particularly important in understanding the molecular basis of enzyme production that could allow a further strain improvement for plant biomass deconstruction.

Vyšlo v časopise: VIB1, a Link between Glucose Signaling and Carbon Catabolite Repression, Is Essential for Plant Cell Wall Degradation by. PLoS Genet 10(8): e32767. doi:10.1371/journal.pgen.1004500
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004500

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