Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis

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At the heart of reproductive cell formation is a nuclear division process (meiosis) whereby homologous chromosomes segregate from one another. Meiotic partner chromosomes establish exclusive associations via a patterned distribution of crossover recombination events. During the maturation of recombination intermediates into crossovers, homologous axes are aligned in the context of a striking proteinaceous structure, the synaptonemal complex (SC). While genetic data link the SC with crossovers, it is unclear whether the mature SC structure facilitates crossover formation. Here we describe an interspecies complementation experiment in which we replace the S. cerevisiae version of an SC structural protein with an ancestrally related version from K. lactis. Our experiment reveals that, while SC proteins are required, mature full-length SC is dispensable for the formation of SC-associated crossovers in budding yeast. We furthermore discovered that most, but not all, members of a conserved meiotic crossover pathway are required for the crossovers that form in this interspecies context. Our findings strengthen the notion that a primary function of many SC proteins is to facilitate crossover recombination, independent of a role in building the larger SC structure. Furthermore, these data suggest that during normal meiosis in S. cerevisiae the assembled SC may act to functionally couple key crossover recombination proteins to one another.

Vyšlo v časopise: Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet 11(6): e32767. doi:10.1371/journal.pgen.1005335
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005335

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