Complement as an Endogenous Adjuvant for Dendritic Cell-Mediated Induction of Retrovirus-Specific CTLs
Previous studies have demonstrated the involvement of complement (C) in induction of efficient CTL responses against different viral infections, but the exact role of complement in this process has not been determined. We now show that C opsonization of retroviral particles enhances the ability of dendritic cells (DCs) to induce CTL responses both in vitro and in vivo. DCs exposed to C-opsonized HIV in vitro were able to stimulate CTLs to elicit antiviral activity significantly better than non-opsonized HIV. Furthermore, experiments using the Friend virus (FV) mouse model illustrated that the enhancing role of complement on DC-mediated CTL induction also occurred in vivo. Our results indicate that complement serves as natural adjuvant for DC-induced expansion and differentiation of specific CTLs against retroviruses.
Vyšlo v časopise:
Complement as an Endogenous Adjuvant for Dendritic Cell-Mediated Induction of Retrovirus-Specific CTLs. PLoS Pathog 6(4): e32767. doi:10.1371/journal.ppat.1000891
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1000891
Souhrn
Previous studies have demonstrated the involvement of complement (C) in induction of efficient CTL responses against different viral infections, but the exact role of complement in this process has not been determined. We now show that C opsonization of retroviral particles enhances the ability of dendritic cells (DCs) to induce CTL responses both in vitro and in vivo. DCs exposed to C-opsonized HIV in vitro were able to stimulate CTLs to elicit antiviral activity significantly better than non-opsonized HIV. Furthermore, experiments using the Friend virus (FV) mouse model illustrated that the enhancing role of complement on DC-mediated CTL induction also occurred in vivo. Our results indicate that complement serves as natural adjuvant for DC-induced expansion and differentiation of specific CTLs against retroviruses.
Zdroje
1. PantaleoG
DemarestJF
SoudeynsH
GraziosiC
DenisF
1994 Major expansion of CD8+ T cells with a predominant V beta usage during the primary immune response to HIV. Nature 370 463 467
2. BorrowP
LewickiH
HahnBH
ShawGM
OldstoneMB
1994 Virus-specific CD8+ cytotoxic T-lymphocyte activity associated with control of viremia in primary human immunodeficiency virus type 1 infection. J Virol 68 6103 6110
3. KoupRA
SafritJT
CaoY
AndrewsCA
McLeodG
1994 Temporal association of cellular immune responses with the initial control of viremia in primary human immunodeficiency virus type 1 syndrome. J Virol 68 4650 4655
4. McMichaelAJ
Rowland-JonesSL
2001 Cellular immune responses to HIV. Nature 410 980 987
5. DeeksSG
WalkerBD
2007 Human immunodeficiency virus controllers: mechanisms of durable virus control in the absence of antiretroviral therapy. Immunity 27 406 416
6. BettsMR
GrayCM
CoxJH
FerrariG
2006 Antigen-specific T-cell-mediated immunity after HIV-1 infection: implications for vaccine control of HIV development. Expert Rev Vaccines 5 505 516
7. MehlhopE
WhitbyK
OliphantT
MarriA
EngleM
DiamondMS
2005 Complement activation is required for induction of a protective antibody response against West Nile virus infection. J Virol 79 7466 7477
8. Da CostaXJ
BrockmanMA
AlicotE
MaM
FischerMB
1999 Humoral response to herpes simplex virus is complement-dependent. Proc Natl Acad Sci U S A 96 12708 12712
9. VerschoorA
BrockmanMA
KnipeDM
CarrollMC
2001 Cutting edge: myeloid complement C3 enhances the humoral response to peripheral viral infection. J Immunol 167 2446 2451
10. KopfM
AbelB
GallimoreA
CarrollM
BachmannMF
2002 Complement component C3 promotes T cell priming and lung migration to control acute influenza virus infection. Nat Med 8 373 378
11. SureshM
MolinaH
SalvatoMS
MastellosD
LambrisJD
SandorM
2003 Complement component 3 is required for optimal expansion of CD8 T cells during a systemic viral infection. J Immunol 170 788 794
12. MehlhopE
DiamondMS
2006 Protective immune responses against West Nile Virus are primed by distinct complement activation pathways. J Exp Med 203 1371 1381
13. StagerS
AlexanderJ
KirbyAC
BottoM
RooijenNV
2003 Natural antibodies and complement are endogenous adjuvants for vaccine-induced CD8+ T cell responses. Nat Med 9 1287 1292
14. WilflingsederD
BankiZ
GarciaE
PruensterM
PfisterG
2007 IgG opsonization of HIV impedes provirus formation in and infection of dendritic cells and subsequent long-term transfer to T cells. J Immunol 178 7840 7848
15. StoiberH
SoederholmA
WilflingsederD
GusenbauerS
HildgartnerA
2008 Complement and antibodies: a dangerous liaison in HIV infection? Vaccine 8 I79 185
16. PruensterM
WilflingsederD
BánkiZ
AmmannCG
MuellauerB
2005 C-type lectin-independent interaction of complement opsonized HIV with monocyte-derived dendritic cells. Eur J Immunol 35 2691 2698
17. HasenkrugKJ
DittmerU
2007 Immune control and prevention of chronic Friend retrovirus infection. Front Biosci 12 1544 1551
18. DittmerU
HeH
MesserRJ
SchimmerS
OlbrichAR
OhlenC
GreenbergPD
StromnesIM
IwashiroM
SakaguchiS
EvansLH
PetersonKE
2004 Functional impairment of CD8(+) T cells by regulatory T cells during persistent retroviral infection. Immunity 20 293 303
19. ZelinskyyG
KraftAR
SchimmerS
ArndtT
DittmerU
2006 Kinetics of CD8+ effector T cell responses and induced CD4+ regulatory T cell responses during Friend retrovirus infection. Eur J Immunol 36 2658 2670
20. BettsMR
BrenchleyJM
PriceDA
DeRosaSC
DouekDC
2003 Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J Immunol Methods 281 65 78
21. AgnelliniP
WolintP
RehrM
CahenzliJ
KarrerU
2007 Impaired NFAT nuclear translocation results in split exhaustion of virus-specific CD8+ T cell functions during chronic viral infection. Proc Natl Acad Sci U S A 104 4565 4570
22. BalkowS
KruxF
LoserK
BeckerJU
GrabbeS
DittmerU
2007 Friend retrovirus infection of myeloid dendritic cells impairs maturation, prolongs contact to naive T cells, and favors expansion of regulatory T cells. Blood 110 3949 3958
23. HasenkrugKJ
1999 Lymphocyte deficiencies increase susceptibility to friend virus-induced erythroleukemia in Fv-2 genetically resistant mice. J Virol 73 6468 6473
24. BettsMR
PriceDA
BrenchleyJM
LoréK
GuenagaJ
2004 The Functional Profile of Primary Human Antiviral CD8+ T Cell Effector Activity Is Dictated by Cognate Peptide Concentration. J Immunol 172 6407 6417
25. JungS
UnutmazD
WongP
SanoG
De los SantosK
2002 In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens. Immunity 17 211 220
26. ProbstHC
van den BroekM
2005 Priming of CTLs by lymphocytic choriomeningitis virus depends on dendritic cells. J Immunol 174 3920 3924
27. BrowneEP
LittmanDR
2009 Myd88 is required for an antibody response to retroviral infection. PLoS Pathog 5 e1000298
28. MesserRJ
DittmerU
PetersonKE
HasenkrugKJ
2004 Essential role for virus-neutralizing antibodies in sterilizing immunity against Friend retrovirus infection. Proc Natl Acad Sci U S A 101(33) 12260 5
29. CastroFV
TuttAL
WhiteAL
TeelingJL
JamesS
2008 CD11c provides an effective immunotarget for the generation of both CD4 and CD8 T cell responses. Eur J Immunol 38 2263 2273
30. SaronMF
FayolleC
SeboP
LadantD
UllmannA
1997 Anti-viral protection conferred by recombinant adenylate cyclase toxins from Bordetella pertussis carrying a CD8+ T cell epitope from lymphocytic choriomeningitis virus. Proc Natl Acad Sci U S A 94 3314 3319
31. GuermonprezP
KhelefN
BlouinE
RieuP
Ricciardi-CastagnoliP
2001 The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18). J Exp Med 193 1035 1044
32. SimsovaM
SeboP
LeclercC
2004 The adenylate cyclase toxin from Bordetella pertussis–a novel promising vehicle for antigen delivery to dendritic cells. Int J Med Microbiol 293 571 576
33. BehrensEM
SriramU
ShiversDK
GallucciM
MaZ
2007 Complement receptor 3 ligation of dendritic cells suppresses their stimulatory capacity. J Immunol 178 6268 6279
34. SkoberneM
SomersanS
AlmodovarW
TruongT
PetrovaK
HensonPM
BhardwajN
2006 The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function. Blood 108 947 955
35. ThorntonBP
Ve˘tvickaV
RossGD
1994 Natural antibody and complement-mediated antigen processing and presentation by B lymphocytes. J Immunol 1994 Feb 15;152(4) 1727 37
36. LiuF
MboudjekaI
ShenS
ChouTH
WangS
RossTM
LuS
2004 Independent but not synergistic enhancement to the immunogenicity of DNA vaccine expressing HIV-1 gp120 glycoprotein by codon optimization and C3d fusion in a mouse model. Vaccine 22 1764 1772
37. OhlénC
KalosM
ChengLE
ShurAC
HongDJ
2002 CD8(+) T cell tolerance to a tumor-associated antigen is maintained at the level of expansion rather than effector function. J Exp Med 195 1407 1418
38. ChenW
QinH
ChesebroB
CheeverMA
1996 Identification of a gag-encoded cytotoxic T-lymphocyte epitope from FBL-3 leukemia shared by Friend, Moloney, and Rauscher murine leukemia virus-induced tumors. J Virol 70 7773 7782
39. BánkiZ
KacaniL
RusertP
PruensterM
WilflingsederD
2005 Complement dependent trapping of infectious HIV in human lymphoid tissues. AIDS 19 481 486
40. InabaK
InabaM
RomaniN
AyaH
DeguchiM
1992 Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J Exp Med 176 1693 1702
41. RobertsonMN
MiyazawaM
MoriS
CaugheyB
EvansLH
1991 Production of monoclonal antibodies reactive with a denatured form of the Friend murine leukemia virus gp70 envelope protein: use in a focal infectivity assay, immunohistochemical studies, electron microscopy and western blotting. J Virol Methods 34 255 271
Štítky
Hygiena a epidemiológia Infekčné lekárstvo LaboratóriumČlánok vyšiel v časopise
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