A Phenome-Based Functional Analysis of Transcription Factors in the Cereal Head Blight Fungus,

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Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of F. graminearum TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level.

Vyšlo v časopise: A Phenome-Based Functional Analysis of Transcription Factors in the Cereal Head Blight Fungus,. PLoS Pathog 7(10): e32767. doi:10.1371/journal.ppat.1002310
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1002310

Souhrn

Zdroje

1. ShelestE 2008 Transcription factors in fungi. FEMS Microbiol Lett 286 145 151

2. LeslieJFSummerellBA 2006 The Fusarium laboratory manual Ames, IA Blackwell Pub

3. DesjardinsAEProctorRH 2007 Molecular biology of Fusarium mycotoxins. Int J Food Microbiol 119 47 50

4. CuomoCAGuldenerUXuJ-RTrailFTurgeonBG 2007 The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization. Science 317 1400 1402

5. HallenHEHuebnerMShiuS-HGüldenerUTrailF 2007 Gene expression shifts during perithecium development in Gibberella zeae (anamorph Fusarium graminearum), with particular emphasis on ion transport proteins. Fungal Genet Biol 44 1146 1156

6. HallenHETrailF 2008 The L-type calcium ion channel Cch1 affects ascospore discharge and mycelial growth in the filamentous fungus Gibberella zeae (anamorph Fusarium graminearum). Eukaryot Cell 7 415 424

7. LysøeESeongK-YKistlerHC 2011 The transcriptome of Fusarium graminearum during the infection of wheat. Mol Plant Microbe Interact 24 995 1000

8. GuentherJCHallen-AdamsHEBückingHShachar-HillYTrailF 2009 Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat. Mol Plant Microbe Interact 22 1492 1503

9. StephensAEGardinerDMWhiteRGMunnALMannersJM 2008 Phases of infection and gene expression of Fusarium graminearum during crown rot disease of wheat. Mol Plant Microbe Interact 21 1571 1581

10. GardinerDMKazanKMannersJM 2009 Novel genes of Fusarium graminearum that negatively regulate deoxynivalenol production and virulence. Mol Plant Microbe Interact 22 1588 1600

11. SeongK-YZhaoXXuJ-RGüldenerUKistlerHC 2008 Conidial germination in the filamentous fungus Fusarium graminearum. Fungal Genet Biol 45 389 399

12. GüldenerUSeongK-YBodduJChoSTrailF 2006 Development of a Fusarium graminearum Affymetrix GeneChip for profiling fungal gene expression in vitro and in planta. Fungal Genet Biol 43 316 325

13. ParkJParkJJangSKimSKongS 2008 FTFD: an informatics pipeline supporting phylogenomic analysis of fungal transcription factors. Bioinformatics 24 1024 1025

14. KimJ-EJinJKimHKimJ-CYunS-H 2006 GIP2, a putative transcription factor that regulates the aurofusarin biosynthetic gene cluster in Gibberella zeae. Appl Environ Microbiol 72 1645 1652

15. SeongK-YPasqualiMZhouXSongJHilburnK 2009 Global gene regulation by Fusarium transcription factors Tri6 and Tri10 reveals adaptations for toxin biosynthesis. Mol Microbiol 72 354 367

16. KimY-TLeeY-RJinJHanK-HKimH 2005 Two different polyketide synthase genes are required for synthesis of zearalenone in Gibberella zeae. Mol Microbiol 58 1102 1113

17. MerhejJRichard-ForgetFBarreauC 2011 The pH regulatory factor Pac1 regulates Tri gene expression and trichothecene production in Fusarium graminearum. Fungal Genet Biol 48 275 284

18. LeeJLeeTLeeY-WYunS-HTurgeonBG 2003 Shifting fungal reproductive mode by manipulation of mating type genes: obligatory heterothallism of Gibberella zeae. Mol Microbiol 50 145 152

19. LysøeEPasqualiMBreakspearAKistlerHC 2011 The transcription factor FgStuAp influences spore development, pathogenicity, and secondary metabolism in Fusarium graminearum. Mol Plant Microbe Interact 24 54 67

20. SeongK-YHouZTracyMKistlerHCXuJ-R 2005 Random insertional mutagenesis identifies genes associated with virulence in the wheat scab fungus Fusarium graminearum. Phytopathology 95 744 750

21. ShimW-BSagaramUSChoiY-ESoJWilkinsonHH 2006 FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum. Mol Plant Microbe Interact 19 725 733

22. WangYLiuWHouZWangCZhouX 2011 A novel transcriptional factor important for pathogenesis and ascosporogenesis in Fusarium graminearum. Mol Plant Microbe Interact 24 118 128

23. RiechmannJLHeardJMartinGReuberLJiangC-Z 2000 Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes. Science 290 2105 2110

24. SonHLeeJParkARLeeY-W 2011 ATP citrate lyase is required for normal sexual and asexual development in Gibberella zeae. Fungal Genet Biol 48 408 417

25. ColotHVParkGTurnerGERingelbergCCrewCM 2006 A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors. Proc Natl Acad Sci U S A 103 10352 10357

26. ParkJParkBJungKJangSYuK 2008 CFGP: a web-based, comparative fungal genomics platform. Nucleic Acids Res 36 D562 D571

27. SeoY-SChernMBartleyLEHanMJungK-H 2011 Towards establishment of a rice stress response interactome. PLoS Genet 7 e1002020

28. TongAHYEvangelistaMParsonsABXuHBaderGD 2001 Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294 2364 2368

29. BevanMWalshS 2005 The Arabidopsis genome: A foundation for plant research. Genome Res 15 1632 1642

30. BernsKHijmansEMMullendersJBrummelkampTRVeldsA 2004 A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428 431 437

31. AshrafiKChangFYWattsJLFraserAGKamathRS 2003 Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421 268 272

32. WinzelerEAShoemakerDDAstromoffALiangHAndersonK 1999 Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285 901 906

33. KimD-UHaylesJKimDWoodVParkH-O 2010 Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe. Nat Biotech 28 617 623

34. JeonJParkS-YChiM-HChoiJParkJ 2007 Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nat Genet 39 561 565

35. JordanIKRogozinIBWolfYIKooninEV 2002 Essential genes are more evolutionarily conserved than are nonessential genes in bacteria. Genome Res 12 962 968

36. NicholsRJSenSChooYJBeltraoPZietekM 2011 Phenotypic landscape of a bacterial cell. Cell 144 143 156

37. ProctorRHHohnTMMcCormickSP 1995 Reduced virulence of Gibberella zeae caused by disruption of a trichthecene toxin biosynthetic gene. Mol Plant Microbe Interact 8 593 601

38. DesjardinsAEProctorRHBaiGMcCormickSPShanerG 1996 Reduced virulence of trichothecene-nonproducing mutants of Gibberella zeae in wheat field tests. Mol Plant Microbe Interact 9 775 781

39. BowdenRLLeslieJF 1999 Sexual recombination in Gibberella zeae. Phytopathology 89 182 188

40. SambrookJRussellDW 2001 Molecular cloning: a laboratory manual, 2nd ed Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press

41. YuJ-HHamariZHanK-HSeoJ-AReyes-DominguezY 2004 Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet Biol 41 973 981

42. HongS-YSoJLeeJMinKSonH 2010 Functional analyses of two syntaxin-like SNARE genes, GzSYN1 and GzSYN2, in the ascomycete Gibberella zeae. Fungal Genet Biol 47 364 372

43. CatlettNLLeeBNYoderOCTurgeonBG 2003 Split-marker recombination for efficient targeted deletion of fungal genes. Fungal Genet Newsl 50 9 11

44. CappelliniRAPetersonJL 1965 Macroconidium formation in submerged cultures by a non-sporulating strain of Gibberella zeae. Mycologia 57 962 966

45. BaiZHarveyLMMcNeilB 2003 Oxidative stress in submerged cultures of fungi. Crit Rev Biotechnol 23 267 302

46. SeoJ-AKimJ-CLeeD-HLeeY-W 1996 Variation in 8-ketotrichothecenes and zearalenone production by Fusarium graminearum isolates from corn and barley in Korea. Mycopathologia 134 31 37

47. Affymetrix 2001 Affymetrix Microarray Suite User Guide. Version 5 Santa Clara, CA Affymetrix

48. O'BrienKPRemmMSonnhammerELL 2005 Inparanoid: a comprehensive database of eukaryotic orthologs. Nucleic Acids Res 33 D476 D480

49. YuHLuscombeNMLuHXZhuXXiaY 2004 Annotation transfer between genomes: Protein–protein interologs and protein–DNA regulogs. Genome Res 14 1107 1118