-Mediated Detoxification of Reactive Oxygen
Species Is Required for Full Virulence in the Rice Blast Fungus

English version České info

During plant-pathogen interactions, the plant may mount several types of defense

responses to either block the pathogen completely or ameliorate the amount of

disease. Such responses include release of reactive oxygen species (ROS) to

attack the pathogen, as well as formation of cell wall appositions (CWAs) to

physically block pathogen penetration. A successful pathogen will likely have

its own ROS detoxification mechanisms to cope with this inhospitable

environment. Here, we report one such candidate mechanism in the rice blast

fungus, Magnaporthe oryzae, governed by a gene we refer to as

MoHYR1. This gene (MGG_07460) encodes a glutathione

peroxidase (GSHPx) domain, and its homologue in yeast was reported to

specifically detoxify phospholipid peroxides. To characterize this gene in

M. oryzae, we generated a deletion

mutantΔhyr1 which showed growth inhibition with

increased amounts of hydrogen peroxide (H₂O₂). Moreover,

we observed that the fungal mutants had a decreased ability to tolerate ROS

generated by a susceptible plant, including ROS found associated with CWAs.

Ultimately, this resulted in significantly smaller lesion sizes on both barley

and rice. In order to determine how this gene interacts with other (ROS)

scavenging-related genes in M. oryzae, we compared expression

levels of ten genes in mutant versus wild type with and without

H₂O₂. Our results indicated that the

HYR1 gene was important for allowing the fungus to tolerate

H₂O₂

in vitro and in planta and that this ability

was directly related to fungal virulence.

Vyšlo v časopise: -Mediated Detoxification of Reactive Oxygen Species Is Required for Full Virulence in the Rice Blast Fungus. PLoS Pathog 7(4): e32767. doi:10.1371/journal.ppat.1001335
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1001335

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