A Synthetic Community Approach Reveals Plant Genotypes Affecting the Phyllosphere Microbiota

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The leaves of plants are inhabited by a diverse community of microorganisms. These leaf inhabitants influence their hosts with respect to growth and resistance to abiotic and biotic stresses. Recent studies revealed that the bacterial communities associated with leaves undergo selection, resulting in conserved microbial communities. However, the factors that are involved in structuring of bacterial communities are not well understood. In order to uncover host genetic factors that determine the community composition and to exclude confounding environmental effects, we inoculated Arabidopsis thaliana with a synthetic bacterial community under controlled conditions We screened a panel of Arabidopsis mutants defective in various traits for alterations in community structure and abundance and were able to show that cuticle synthesis and ethylene perception affect the bacterial community. In addition, we identified plant ecotypes with drastic differences in the community composition. Our system can thus be used to identify additional host genes and to broaden insights into plant microbe interactions, potentially providing a basis for applied plant protection through the identification of traits that enhance growth of plant probiotic bacteria.

Vyšlo v časopise: A Synthetic Community Approach Reveals Plant Genotypes Affecting the Phyllosphere Microbiota. PLoS Genet 10(4): e32767. doi:10.1371/journal.pgen.1004283
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004283

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