A Novel Highly Divergent Protein Family Identified from a Viviparous Insect by RNA-seq Analysis: A Potential Target for Tsetse Fly-Specific Abortifacients

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Tsetse flies are the sole vector for African trypanosomes, causative agents of sleeping sickness in humans and nagana in cattle. Transcriptome and proteome analyses were utilized to examine the underlying mechanisms of tsetse lactation that occur during each reproductive cycle. These analyses revealed a dramatic shift to the synthesis of milk proteins during lactation and a novel milk-specific protein family. All members of this family were co-localized, shared sequence similarity and were expressed at 40× basal levels during milk secretion. Suppression of gene from this lactation-associated family impaired progeny development by reducing milk protein content and altering milk homeostasis. These novel genes represent an excellent target for tsetse-specific reproductive-based control mechanisms. In addition, the characterization of tsetse milk production revealed multiple factors that are functionally analogous between tsetse and mammalian lactation.

Vyšlo v časopise: A Novel Highly Divergent Protein Family Identified from a Viviparous Insect by RNA-seq Analysis: A Potential Target for Tsetse Fly-Specific Abortifacients. PLoS Genet 10(4): e32767. doi:10.1371/journal.pgen.1003874
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1003874

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