A Novel Peptidoglycan Binding Protein Crucial for PBP1A-Mediated Cell Wall Biogenesis in

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Bacteria surround themselves with a mesh-like peptidoglycan (PG) cell wall, which is essential for maintenance of cell shape and survival. While the enzymes that catalyze the assembly of the cell wall (aka penicillin-binding proteins (PBPs)) have been extensively characterized, our understanding of the factors that modulate the activities of these enzymes is less developed. Here, using a genetic screen, we identified a gene of unknown function that plays a crucial role in PBP1A-mediated cell wall synthesis in Vibrio cholerae, the bacterium causing cholera. V. cholerae mutants lacking this gene, whose protein product was re-named CsiV (for Cell shape integrity Vibrio), share many phenotypes with PBP1A mutants, including becoming spherical during stationary phase. We show that CsiV interacts with LpoA, a lipoprotein activator of PBP1A, as well as with PG. CsiV, LpoA, or PBP1A are all required for survival of V. cholerae lacking PBP1B, and mutants lacking any of these factors show marked changes in PG content in stationary phase. Collectively, our data suggest that CsiV acts through LpoA to promote PG biogenesis in V. cholerae and other vibrio species as well as in the other genera where this protein is found.

Vyšlo v časopise: A Novel Peptidoglycan Binding Protein Crucial for PBP1A-Mediated Cell Wall Biogenesis in. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004433
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004433

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